IAA2_ARATH
ID IAA2_ARATH Reviewed; 174 AA.
AC P49678; O22596;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2001, sequence version 3.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Auxin-responsive protein IAA2;
DE AltName: Full=Indoleacetic acid-induced protein 2;
GN Name=IAA2; OrderedLocusNames=At3g23030; ORFNames=MXC7.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=8278386; DOI=10.1073/pnas.91.1.326;
RA Abel S., Oeller P.W., Theologis A.;
RT "Early auxin-induced genes encode short-lived nuclear proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:326-330(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Wassilewskija;
RA Malik M.K., Zimmerman J.L.;
RT "Molecular characterization of a genomic clone encoding the auxin-inducible
RT IAA2 gene from Arabidopsis thaliana.";
RL (er) Plant Gene Register PGR97-178(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=7658471; DOI=10.1006/jmbi.1995.0454;
RA Abel S., Nguyen M.D., Theologis A.;
RT "The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis
RT thaliana.";
RL J. Mol. Biol. 251:533-549(1995).
RN [7]
RP DIMERIZATION.
RX PubMed=9342315; DOI=10.1073/pnas.94.22.11786;
RA Kim J., Harter K., Theologis A.;
RT "Protein-protein interactions among the Aux/IAA proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:11786-11791(1997).
RN [8]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=12036262; DOI=10.1023/a:1015255030047;
RA Liscum E., Reed J.W.;
RT "Genetics of Aux/IAA and ARF action in plant growth and development.";
RL Plant Mol. Biol. 49:387-400(2002).
RN [9]
RP TRANSCRIPTIONAL REPRESSION DOMAIN.
RX PubMed=14742873; DOI=10.1105/tpc.017384;
RA Tiwari S.B., Hagen G., Guilfoyle T.J.;
RT "Aux/IAA proteins contain a potent transcriptional repression domain.";
RL Plant Cell 16:533-543(2004).
RN [10]
RP INTERACTION WITH TPL.
RX PubMed=18258861; DOI=10.1126/science.1151461;
RA Szemenyei H., Hannon M., Long J.A.;
RT "TOPLESS mediates auxin-dependent transcriptional repression during
RT Arabidopsis embryogenesis.";
RL Science 319:1384-1386(2008).
CC -!- FUNCTION: Aux/IAA proteins are short-lived transcriptional factors that
CC function as repressors of early auxin response genes at low auxin
CC concentrations. Repression is thought to result from the interaction
CC with auxin response factors (ARFs), proteins that bind to the auxin-
CC responsive promoter element (AuxRE). Formation of heterodimers with ARF
CC proteins may alter their ability to modulate early auxin response genes
CC expression. {ECO:0000269|PubMed:12036262}.
CC -!- SUBUNIT: Homodimers and heterodimers. Interacts with the auxin-
CC responsive protein IAA1. Interacts with TPL.
CC {ECO:0000269|PubMed:18258861}.
CC -!- INTERACTION:
CC P49678; Q8RYC8: ARF19; NbExp=3; IntAct=EBI-632343, EBI-529887;
CC P49678; Q9FGV1: ARF8; NbExp=3; IntAct=EBI-632343, EBI-1554169;
CC P49678; P49677: IAA1; NbExp=13; IntAct=EBI-632343, EBI-630505;
CC P49678; Q38828: IAA10; NbExp=12; IntAct=EBI-632343, EBI-3946434;
CC P49678; Q38829: IAA11; NbExp=6; IntAct=EBI-632343, EBI-2367923;
CC P49678; Q38830: IAA12; NbExp=5; IntAct=EBI-632343, EBI-617608;
CC P49678; Q38831: IAA13; NbExp=8; IntAct=EBI-632343, EBI-1554143;
CC P49678; Q38832: IAA14; NbExp=4; IntAct=EBI-632343, EBI-2295562;
CC P49678; A0A2H1ZEF6: IAA15; NbExp=3; IntAct=EBI-632343, EBI-25524519;
CC P49678; O24407: IAA16; NbExp=10; IntAct=EBI-632343, EBI-632231;
CC P49678; P93830: IAA17; NbExp=10; IntAct=EBI-632343, EBI-632243;
CC P49678; O24408: IAA18; NbExp=4; IntAct=EBI-632343, EBI-2295525;
CC P49678; O24409: IAA19; NbExp=10; IntAct=EBI-632343, EBI-632257;
CC P49678; P49678: IAA2; NbExp=5; IntAct=EBI-632343, EBI-632343;
CC P49678; O24410: IAA20; NbExp=6; IntAct=EBI-632343, EBI-632272;
CC P49678; Q8LAL2: IAA26; NbExp=10; IntAct=EBI-632343, EBI-3947418;
CC P49678; Q9ZSY8: IAA27; NbExp=9; IntAct=EBI-632343, EBI-3946677;
CC P49678; Q9XFM0: IAA28; NbExp=10; IntAct=EBI-632343, EBI-3133404;
CC P49678; Q38822: IAA3; NbExp=12; IntAct=EBI-632343, EBI-307174;
CC P49678; Q8RYC6: IAA32; NbExp=3; IntAct=EBI-632343, EBI-3946448;
CC P49678; Q9C5X0: IAA34; NbExp=6; IntAct=EBI-632343, EBI-3946459;
CC P49678; P33077: IAA4; NbExp=9; IntAct=EBI-632343, EBI-632187;
CC P49678; P33078: IAA5; NbExp=7; IntAct=EBI-632343, EBI-3946487;
CC P49678; Q38824: IAA6; NbExp=7; IntAct=EBI-632343, EBI-1554124;
CC P49678; Q38825: IAA7; NbExp=7; IntAct=EBI-632343, EBI-602959;
CC P49678; Q38826: IAA8; NbExp=7; IntAct=EBI-632343, EBI-632200;
CC P49678; Q38827: IAA9; NbExp=4; IntAct=EBI-632343, EBI-632216;
CC P49678; Q5XEN5: MBD1; NbExp=7; IntAct=EBI-632343, EBI-4445335;
CC P49678; Q9LYB9: MBD4; NbExp=4; IntAct=EBI-632343, EBI-15191715;
CC P49678; Q9C8Y3: RGL1; NbExp=3; IntAct=EBI-632343, EBI-963647;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Preferentially expressed in vegetative organs.
CC {ECO:0000269|PubMed:7658471}.
CC -!- INDUCTION: By auxin. {ECO:0000269|PubMed:7658471}.
CC -!- DOMAIN: The N-terminal half of the protein contains two conserved
CC domains I and II. Domain I includes a slightly degenerated ERF-
CC associated amphiphilic repression (EAR) motif which seems to be
CC involved in the activity of transcriptional repression. Domain II is
CC required for the correct degradation of the protein through the SCF-
CC mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA
CC proteins and auxin response factors (ARFs) occur through their C-
CC terminal dimerization domains III and IV.
CC -!- SIMILARITY: Belongs to the Aux/IAA family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L15449; AAA16570.1; -; mRNA.
DR EMBL; AF027157; AAB97164.1; -; Genomic_DNA.
DR EMBL; AB026655; BAB02094.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76707.1; -; Genomic_DNA.
DR EMBL; AF332392; AAG48756.1; -; mRNA.
DR RefSeq; NP_188943.1; NM_113203.5.
DR AlphaFoldDB; P49678; -.
DR SMR; P49678; -.
DR BioGRID; 7209; 52.
DR ELM; P49678; -.
DR IntAct; P49678; 48.
DR STRING; 3702.AT3G23030.1; -.
DR PaxDb; P49678; -.
DR ProteomicsDB; 232142; -.
DR EnsemblPlants; AT3G23030.1; AT3G23030.1; AT3G23030.
DR GeneID; 821877; -.
DR Gramene; AT3G23030.1; AT3G23030.1; AT3G23030.
DR KEGG; ath:AT3G23030; -.
DR Araport; AT3G23030; -.
DR TAIR; locus:2094578; AT3G23030.
DR eggNOG; ENOG502QU81; Eukaryota.
DR HOGENOM; CLU_049393_0_1_1; -.
DR InParanoid; P49678; -.
DR PhylomeDB; P49678; -.
DR PRO; PR:P49678; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; P49678; baseline and differential.
DR Genevisible; P49678; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009733; P:response to auxin; IEP:TAIR.
DR InterPro; IPR033389; AUX/IAA_dom.
DR InterPro; IPR003311; AUX_IAA.
DR InterPro; IPR000270; PB1_dom.
DR PANTHER; PTHR31734; PTHR31734; 1.
DR Pfam; PF02309; AUX_IAA; 1.
DR PROSITE; PS51745; PB1; 1.
PE 1: Evidence at protein level;
KW Auxin signaling pathway; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..174
FT /note="Auxin-responsive protein IAA2"
FT /id="PRO_0000112833"
FT DOMAIN 77..164
FT /note="PB1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT REGION 44..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 16..20
FT /note="EAR-like (transcriptional repression)"
FT CONFLICT 38
FT /note="S -> I (in Ref. 2; AAB97164)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 174 AA; 19910 MW; DECA4F59F0106179 CRC64;
MAYEKVNELN LKDTELCLGL PGRTEKIKEE QEVSCVKSNN KRLFEETRDE EESTPPTKTQ
IVGWPPVRSS RKNNNSVSYV KVSMDGAPYL RKIDLKTYKN YPELLKALEN MFKVMIGEYC
EREGYKGSGF VPTYEDKDGD WMLVGDVPWD MFSSSCKRLR IMKGSDAPAL DSSL
