ID   IAA3_WHEAT              Reviewed;          44 AA.
AC   P10846;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Alpha-amylase inhibitor WDAI-3;
DE   Flags: Fragment;
GN   Name=IHA-B1-2;
OS   Triticum aestivum (Wheat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX   NCBI_TaxID=4565;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=cv. Chinese Spring;
RX   PubMed=2787745; DOI=10.1111/j.1432-1033.1989.tb14893.x;
RA   Sanchez-Monge R., Gomez L., Garcia-Olmedo F., Salcedo G.;
RT   "New dimeric inhibitor of heterologous alpha-amylases encoded by a
RT   duplicated gene in the short arm of chromosome 3B of wheat (Triticum
RT   aestivum L.).";
RL   Eur. J. Biochem. 183:37-40(1989).
CC   -!- FUNCTION: Alpha-amylase inhibitor.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Endosperm.
CC   -!- PTM: The disulfide bonds are essential for the inhibitor activity.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I6 (cereal trypsin/alpha-
CC       amylase inhibitor) family. {ECO:0000305}.
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DR   AlphaFoldDB; P10846; -.
DR   SMR; P10846; -.
DR   PRIDE; P10846; -.
DR   Proteomes; UP000019116; Unplaced.
DR   ExpressionAtlas; P10846; baseline.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015066; F:alpha-amylase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:InterPro.
DR   Gene3D; 1.10.110.10; -; 1.
DR   InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib.
DR   InterPro; IPR006105; Allergen/tryp_amyl_inhib_CS.
DR   InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR   PRINTS; PR00808; AMLASEINHBTR.
DR   SUPFAM; SSF47699; SSF47699; 1.
DR   PROSITE; PS00426; CEREAL_TRYP_AMYL_INH; 1.
PE   1: Evidence at protein level;
KW   Alpha-amylase inhibitor; Direct protein sequencing; Disulfide bond;
KW   Reference proteome; Secreted.
FT   CHAIN           1..>44
FT                   /note="Alpha-amylase inhibitor WDAI-3"
FT                   /id="PRO_0000070487"
FT   DISULFID        20..41
FT                   /evidence="ECO:0000250"
FT   DISULFID        28..?
FT                   /evidence="ECO:0000250"
FT   DISULFID        42..?
FT                   /evidence="ECO:0000250"
FT   NON_TER         44
SQ   SEQUENCE   44 AA;  4797 MW;  09B844C5530DC73F CRC64;
     SGPWMCYPGY AFKVPALPGC RPVLLLQCNG SQVPEAVLRD CCQQ