IAA4_ARATH
ID IAA4_ARATH Reviewed; 186 AA.
AC P33077;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Auxin-responsive protein IAA4;
DE AltName: Full=Auxin-induced protein AUX2-11;
DE AltName: Full=Indoleacetic acid-induced protein 4;
GN Name=IAA4; Synonyms=AUX2-11; OrderedLocusNames=At5g43700; ORFNames=MQD19.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=2102379; DOI=10.1007/bf00017836;
RA Conner T.W., Goekjian V.H., Lafayette P.R., Key J.L.;
RT "Structure and expression of two auxin-inducible genes from Arabidopsis.";
RL Plant Mol. Biol. 15:623-632(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=8278386; DOI=10.1073/pnas.91.1.326;
RA Abel S., Oeller P.W., Theologis A.;
RT "Early auxin-induced genes encode short-lived nuclear proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:326-330(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=7658471; DOI=10.1006/jmbi.1995.0454;
RA Abel S., Nguyen M.D., Theologis A.;
RT "The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis
RT thaliana.";
RL J. Mol. Biol. 251:533-549(1995).
RN [7]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=12036262; DOI=10.1023/a:1015255030047;
RA Liscum E., Reed J.W.;
RT "Genetics of Aux/IAA and ARF action in plant growth and development.";
RL Plant Mol. Biol. 49:387-400(2002).
RN [8]
RP TRANSCRIPTIONAL REPRESSION DOMAIN.
RX PubMed=14742873; DOI=10.1105/tpc.017384;
RA Tiwari S.B., Hagen G., Guilfoyle T.J.;
RT "Aux/IAA proteins contain a potent transcriptional repression domain.";
RL Plant Cell 16:533-543(2004).
RN [9]
RP INTERACTION WITH TPL.
RX PubMed=18258861; DOI=10.1126/science.1151461;
RA Szemenyei H., Hannon M., Long J.A.;
RT "TOPLESS mediates auxin-dependent transcriptional repression during
RT Arabidopsis embryogenesis.";
RL Science 319:1384-1386(2008).
CC -!- FUNCTION: Aux/IAA proteins are short-lived transcriptional factors that
CC function as repressors of early auxin response genes at low auxin
CC concentrations. Repression is thought to result from the interaction
CC with auxin response factors (ARFs), proteins that bind to the auxin-
CC responsive promoter element (AuxRE). Formation of heterodimers with ARF
CC proteins may alter their ability to modulate early auxin response genes
CC expression. {ECO:0000269|PubMed:12036262}.
CC -!- SUBUNIT: Homodimers and heterodimers (By similarity). Interacts with
CC TPL. {ECO:0000250, ECO:0000269|PubMed:18258861}.
CC -!- INTERACTION:
CC P33077; Q8RYC8: ARF19; NbExp=3; IntAct=EBI-632187, EBI-529887;
CC P33077; P49677: IAA1; NbExp=10; IntAct=EBI-632187, EBI-630505;
CC P33077; Q38828: IAA10; NbExp=10; IntAct=EBI-632187, EBI-3946434;
CC P33077; Q38829: IAA11; NbExp=7; IntAct=EBI-632187, EBI-2367923;
CC P33077; Q38830: IAA12; NbExp=6; IntAct=EBI-632187, EBI-617608;
CC P33077; Q38831: IAA13; NbExp=9; IntAct=EBI-632187, EBI-1554143;
CC P33077; Q38832: IAA14; NbExp=4; IntAct=EBI-632187, EBI-2295562;
CC P33077; A0A2H1ZEF6: IAA15; NbExp=5; IntAct=EBI-632187, EBI-25524519;
CC P33077; O24407: IAA16; NbExp=9; IntAct=EBI-632187, EBI-632231;
CC P33077; P93830: IAA17; NbExp=8; IntAct=EBI-632187, EBI-632243;
CC P33077; O24408: IAA18; NbExp=4; IntAct=EBI-632187, EBI-2295525;
CC P33077; O24409: IAA19; NbExp=9; IntAct=EBI-632187, EBI-632257;
CC P33077; P49678: IAA2; NbExp=9; IntAct=EBI-632187, EBI-632343;
CC P33077; O24410: IAA20; NbExp=6; IntAct=EBI-632187, EBI-632272;
CC P33077; Q8LAL2: IAA26; NbExp=8; IntAct=EBI-632187, EBI-3947418;
CC P33077; Q9ZSY8: IAA27; NbExp=9; IntAct=EBI-632187, EBI-3946677;
CC P33077; Q9XFM0: IAA28; NbExp=9; IntAct=EBI-632187, EBI-3133404;
CC P33077; Q38822: IAA3; NbExp=9; IntAct=EBI-632187, EBI-307174;
CC P33077; Q8H174: IAA31; NbExp=3; IntAct=EBI-632187, EBI-3946408;
CC P33077; Q8RYC6: IAA32; NbExp=5; IntAct=EBI-632187, EBI-3946448;
CC P33077; Q9FKM7: IAA33; NbExp=3; IntAct=EBI-632187, EBI-3946739;
CC P33077; Q9C5X0: IAA34; NbExp=6; IntAct=EBI-632187, EBI-3946459;
CC P33077; P33077: IAA4; NbExp=4; IntAct=EBI-632187, EBI-632187;
CC P33077; P33078: IAA5; NbExp=6; IntAct=EBI-632187, EBI-3946487;
CC P33077; Q38824: IAA6; NbExp=10; IntAct=EBI-632187, EBI-1554124;
CC P33077; Q38826: IAA8; NbExp=7; IntAct=EBI-632187, EBI-632200;
CC P33077; Q38827: IAA9; NbExp=4; IntAct=EBI-632187, EBI-632216;
CC P33077; O65154: KIWI; NbExp=3; IntAct=EBI-632187, EBI-2112286;
CC P33077; O23160: MYB73; NbExp=3; IntAct=EBI-632187, EBI-25506855;
CC P33077; Q9LXU1: PIM1; NbExp=4; IntAct=EBI-632187, EBI-15193025;
CC P33077; Q9C8Y3: RGL1; NbExp=3; IntAct=EBI-632187, EBI-963647;
CC P33077; Q9FTA2: TCP21; NbExp=3; IntAct=EBI-632187, EBI-4426168;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Preferentially expressed in stems, leaves and
CC flowers. {ECO:0000269|PubMed:7658471}.
CC -!- INDUCTION: By auxin. {ECO:0000269|PubMed:7658471}.
CC -!- DOMAIN: The N-terminal half of the protein contains two conserved
CC domains I and II. Domain I includes a slightly degenerated ERF-
CC associated amphiphilic repression (EAR) motif which seems to be
CC involved in the activity of transcriptional repression. Domain II is
CC required for the correct degradation of the protein through the SCF-
CC mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA
CC proteins and auxin response factors (ARFs) occur through their C-
CC terminal dimerization domains III and IV.
CC -!- SIMILARITY: Belongs to the Aux/IAA family. {ECO:0000305}.
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DR EMBL; X53435; CAA37526.1; -; Genomic_DNA.
DR EMBL; L15450; AAA16571.1; -; mRNA.
DR EMBL; AB026651; BAB11297.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94998.1; -; Genomic_DNA.
DR EMBL; BT005853; AAO64788.1; -; mRNA.
DR PIR; S12243; S12243.
DR RefSeq; NP_199183.1; NM_123736.3.
DR AlphaFoldDB; P33077; -.
DR SMR; P33077; -.
DR BioGRID; 19640; 49.
DR ELM; P33077; -.
DR IntAct; P33077; 42.
DR STRING; 3702.AT5G43700.1; -.
DR PaxDb; P33077; -.
DR PRIDE; P33077; -.
DR ProteomicsDB; 232094; -.
DR EnsemblPlants; AT5G43700.1; AT5G43700.1; AT5G43700.
DR GeneID; 834390; -.
DR Gramene; AT5G43700.1; AT5G43700.1; AT5G43700.
DR KEGG; ath:AT5G43700; -.
DR Araport; AT5G43700; -.
DR TAIR; locus:2170862; AT5G43700.
DR eggNOG; ENOG502QU81; Eukaryota.
DR HOGENOM; CLU_049393_0_1_1; -.
DR InParanoid; P33077; -.
DR OMA; ENMFKLS; -.
DR OrthoDB; 1265494at2759; -.
DR PhylomeDB; P33077; -.
DR PRO; PR:P33077; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; P33077; baseline and differential.
DR Genevisible; P33077; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; ISS:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009733; P:response to auxin; IEP:TAIR.
DR InterPro; IPR033389; AUX/IAA_dom.
DR InterPro; IPR003311; AUX_IAA.
DR InterPro; IPR000270; PB1_dom.
DR PANTHER; PTHR31734; PTHR31734; 1.
DR Pfam; PF02309; AUX_IAA; 1.
DR PROSITE; PS51745; PB1; 1.
PE 1: Evidence at protein level;
KW Auxin signaling pathway; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..186
FT /note="Auxin-responsive protein IAA4"
FT /id="PRO_0000112835"
FT DOMAIN 88..175
FT /note="PB1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT REGION 25..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 18..22
FT /note="EAR-like (transcriptional repression)"
SQ SEQUENCE 186 AA; 20975 MW; DCF66CAC4D28CD3B CRC64;
MEKVDVYDEL VNLKATELRL GLPGTEETVS CGKSNKRVLP EATEKEIEST GKTETASPPK
AQIVGWPPVR SYRKNNVQTK KSESEGQGNY VKVSMDGAPY LRKIDLTMYK QYPELMKSLE
NMFKFSVGEY FEREGYKGSD FVPTYEDKDG DWMLVGDVPW EMFVSSCKRL RIMKGSEVKG
LGCGGL
