ID   IAA4_SORBI              Reviewed;         118 AA.
AC   P81367;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Alpha-amylase inhibitor 4;
DE   AltName: Full=SI alpha-4;
OS   Sorghum bicolor (Sorghum) (Sorghum vulgare).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Sorghinae; Sorghum.
OX   NCBI_TaxID=4558;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=cv. French red; TISSUE=Seed;
RX   PubMed=1492093;
RA   Bloch C. Jr., Richardson M.;
RT   "The amino acid sequences of two 13-kDa alpha-amylase inhibitors from the
RT   seeds of Sorghum bicolor (L.) Moench.";
RL   Protein Seq. Data Anal. 5:27-30(1992).
CC   -!- FUNCTION: Alpha-amylase inhibitor.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I6 (cereal trypsin/alpha-
CC       amylase inhibitor) family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   PIR; S28201; S28201.
DR   AlphaFoldDB; P81367; -.
DR   SMR; P81367; -.
DR   MEROPS; I06.002; -.
DR   ExpressionAtlas; P81367; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015066; F:alpha-amylase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:InterPro.
DR   CDD; cd00261; AAI_SS; 1.
DR   Gene3D; 1.10.110.10; -; 1.
DR   InterPro; IPR044723; AAI_SS_dom.
DR   InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib.
DR   InterPro; IPR006105; Allergen/tryp_amyl_inhib_CS.
DR   InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR   InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR   Pfam; PF00234; Tryp_alpha_amyl; 1.
DR   PRINTS; PR00808; AMLASEINHBTR.
DR   SMART; SM00499; AAI; 1.
DR   SUPFAM; SSF47699; SSF47699; 1.
DR   PROSITE; PS00426; CEREAL_TRYP_AMYL_INH; 1.
PE   1: Evidence at protein level;
KW   Alpha-amylase inhibitor; Direct protein sequencing; Disulfide bond;
KW   Secreted.
FT   CHAIN           1..118
FT                   /note="Alpha-amylase inhibitor 4"
FT                   /id="PRO_0000070493"
FT   DISULFID        7..60
FT                   /evidence="ECO:0000250"
FT   DISULFID        21..49
FT                   /evidence="ECO:0000250"
FT   DISULFID        30..82
FT                   /evidence="ECO:0000250"
FT   DISULFID        50..101
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   118 AA;  12500 MW;  B9571100591806BF CRC64;
     TVDVTACAPG LAIPAPPLPT CRTFARPRTC GLGGPYGPVD PSPVLKQRCC RELAAVPSRC
     RCAALGFMMD GVDAPLQDFR GCTREMQRIY AVSRLTRAAE CNLPTIPGGG CHLSNSPR