IAA5_ARATH
ID IAA5_ARATH Reviewed; 163 AA.
AC P33078; Q38823; Q8H1E0;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 3.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Auxin-responsive protein IAA5;
DE AltName: Full=Auxin-induced protein AUX2-27;
DE AltName: Full=Indoleacetic acid-induced protein 5;
GN Name=IAA5; Synonyms=AUX2-27; OrderedLocusNames=At1g15580;
GN ORFNames=T16N11.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=2102379; DOI=10.1007/bf00017836;
RA Conner T.W., Goekjian V.H., Lafayette P.R., Key J.L.;
RT "Structure and expression of two auxin-inducible genes from Arabidopsis.";
RL Plant Mol. Biol. 15:623-632(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 6-163, TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Columbia;
RX PubMed=7658471; DOI=10.1006/jmbi.1995.0454;
RA Abel S., Nguyen M.D., Theologis A.;
RT "The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis
RT thaliana.";
RL J. Mol. Biol. 251:533-549(1995).
RN [6]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=12036262; DOI=10.1023/a:1015255030047;
RA Liscum E., Reed J.W.;
RT "Genetics of Aux/IAA and ARF action in plant growth and development.";
RL Plant Mol. Biol. 49:387-400(2002).
RN [7]
RP TRANSCRIPTIONAL REPRESSION DOMAIN.
RX PubMed=14742873; DOI=10.1105/tpc.017384;
RA Tiwari S.B., Hagen G., Guilfoyle T.J.;
RT "Aux/IAA proteins contain a potent transcriptional repression domain.";
RL Plant Cell 16:533-543(2004).
CC -!- FUNCTION: Aux/IAA proteins are short-lived transcriptional factors that
CC function as repressors of early auxin response genes at low auxin
CC concentrations. Repression is thought to result from the interaction
CC with auxin response factors (ARFs), proteins that bind to the auxin-
CC responsive promoter element (AuxRE). Formation of heterodimers with ARF
CC proteins may alter their ability to modulate early auxin response genes
CC expression. {ECO:0000269|PubMed:12036262}.
CC -!- SUBUNIT: Homodimers and heterodimers. {ECO:0000250}.
CC -!- INTERACTION:
CC P33078; Q93YR9: ARF16; NbExp=4; IntAct=EBI-3946487, EBI-3947588;
CC P33078; Q8RYC8: ARF19; NbExp=3; IntAct=EBI-3946487, EBI-529887;
CC P33078; Q9FIK2: At5g47790; NbExp=3; IntAct=EBI-3946487, EBI-25523851;
CC P33078; P49677: IAA1; NbExp=6; IntAct=EBI-3946487, EBI-630505;
CC P33078; Q38828: IAA10; NbExp=6; IntAct=EBI-3946487, EBI-3946434;
CC P33078; Q38831: IAA13; NbExp=6; IntAct=EBI-3946487, EBI-1554143;
CC P33078; Q38832: IAA14; NbExp=4; IntAct=EBI-3946487, EBI-2295562;
CC P33078; A0A2H1ZEF6: IAA15; NbExp=3; IntAct=EBI-3946487, EBI-25524519;
CC P33078; O24407: IAA16; NbExp=7; IntAct=EBI-3946487, EBI-632231;
CC P33078; P93830: IAA17; NbExp=9; IntAct=EBI-3946487, EBI-632243;
CC P33078; O24409: IAA19; NbExp=6; IntAct=EBI-3946487, EBI-632257;
CC P33078; P49678: IAA2; NbExp=7; IntAct=EBI-3946487, EBI-632343;
CC P33078; O24410: IAA20; NbExp=4; IntAct=EBI-3946487, EBI-632272;
CC P33078; Q8LAL2: IAA26; NbExp=5; IntAct=EBI-3946487, EBI-3947418;
CC P33078; Q9ZSY8: IAA27; NbExp=6; IntAct=EBI-3946487, EBI-3946677;
CC P33078; Q9XFM0: IAA28; NbExp=6; IntAct=EBI-3946487, EBI-3133404;
CC P33078; Q38822: IAA3; NbExp=6; IntAct=EBI-3946487, EBI-307174;
CC P33078; Q8H174: IAA31; NbExp=6; IntAct=EBI-3946487, EBI-3946408;
CC P33078; Q9C5X0: IAA34; NbExp=6; IntAct=EBI-3946487, EBI-3946459;
CC P33078; P33077: IAA4; NbExp=6; IntAct=EBI-3946487, EBI-632187;
CC P33078; Q38824: IAA6; NbExp=5; IntAct=EBI-3946487, EBI-1554124;
CC P33078; Q38826: IAA8; NbExp=7; IntAct=EBI-3946487, EBI-632200;
CC P33078; O22179: MYB70; NbExp=3; IntAct=EBI-3946487, EBI-1238013;
CC P33078; O23160: MYB73; NbExp=3; IntAct=EBI-3946487, EBI-25506855;
CC P33078; Q9C9L2: TCP15; NbExp=3; IntAct=EBI-3946487, EBI-4426144;
CC P33078; Q9MAH8: TCP3; NbExp=3; IntAct=EBI-3946487, EBI-25522447;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Highly expressed in stems and flowers.
CC {ECO:0000269|PubMed:7658471}.
CC -!- INDUCTION: By auxin. {ECO:0000269|PubMed:7658471}.
CC -!- DOMAIN: The N-terminal half of the protein contains two conserved
CC domains I and II. Domain I includes a slightly degenerated ERF-
CC associated amphiphilic repression (EAR) motif which seems to be
CC involved in the activity of transcriptional repression. Domain II is
CC required for the correct degradation of the protein through the SCF-
CC mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA
CC proteins and auxin response factors (ARFs) occur through their C-
CC terminal dimerization domains III and IV.
CC -!- SIMILARITY: Belongs to the Aux/IAA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA37527.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X53436; CAA37527.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC013453; AAF71983.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29337.1; -; Genomic_DNA.
DR EMBL; AY150496; AAN13012.1; -; mRNA.
DR EMBL; U18407; AAC49046.1; -; mRNA.
DR PIR; G86289; G86289.
DR PIR; S12244; S12244.
DR PIR; S58492; S58492.
DR RefSeq; NP_173011.1; NM_101427.5.
DR AlphaFoldDB; P33078; -.
DR SMR; P33078; -.
DR BioGRID; 23368; 61.
DR ELM; P33078; -.
DR IntAct; P33078; 49.
DR STRING; 3702.AT1G15580.1; -.
DR PaxDb; P33078; -.
DR PRIDE; P33078; -.
DR EnsemblPlants; AT1G15580.1; AT1G15580.1; AT1G15580.
DR GeneID; 838128; -.
DR Gramene; AT1G15580.1; AT1G15580.1; AT1G15580.
DR KEGG; ath:AT1G15580; -.
DR Araport; AT1G15580; -.
DR TAIR; locus:2196573; AT1G15580.
DR eggNOG; ENOG502RXTN; Eukaryota.
DR HOGENOM; CLU_049393_0_1_1; -.
DR InParanoid; P33078; -.
DR OMA; CVPIYED; -.
DR OrthoDB; 1334250at2759; -.
DR PhylomeDB; P33078; -.
DR PRO; PR:P33078; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P33078; baseline and differential.
DR Genevisible; P33078; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009733; P:response to auxin; IEP:TAIR.
DR InterPro; IPR033389; AUX/IAA_dom.
DR InterPro; IPR003311; AUX_IAA.
DR InterPro; IPR000270; PB1_dom.
DR PANTHER; PTHR31734; PTHR31734; 1.
DR Pfam; PF02309; AUX_IAA; 1.
DR PROSITE; PS51745; PB1; 1.
PE 1: Evidence at protein level;
KW Auxin signaling pathway; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..163
FT /note="Auxin-responsive protein IAA5"
FT /id="PRO_0000112836"
FT DOMAIN 74..160
FT /note="PB1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT MOTIF 15..19
FT /note="EAR-like (transcriptional repression)"
FT CONFLICT 6..7
FT /note="NN -> GR (in Ref. 5; AAC49046)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 163 AA; 18354 MW; 4B55212FADF344B2 CRC64;
MANESNNLGL EITELRLGLP GDIVVSGESI SGKKRASPEV EIDLKCEPAK KSQVVGWPPV
CSYRRKNSLE RTKSSYVKVS VDGAAFLRKI DLEMYKCYQD LASALQILFG CYINFDDTLK
ESECVPIYED KDGDWMLAGD VPWEMFLGSC KRLRIMKRSC NRG
