ID   IAA5_WHEAT              Reviewed;         124 AA.
AC   P01084;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=Alpha-amylase inhibitor 0.53;
OS   Triticum aestivum (Wheat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX   NCBI_TaxID=4565;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=6186287; DOI=10.1016/0167-4838(83)90417-x;
RA   Maeda K., Hase T., Matsubara H.;
RT   "Complete amino acid sequence of an alpha-amylase inhibitor in wheat
RT   kernel.";
RL   Biochim. Biophys. Acta 743:52-57(1983).
RN   [2]
RP   SEQUENCE REVISION TO 119-124.
RX   PubMed=3872681; DOI=10.1016/0167-4838(85)90299-7;
RA   Maeda K., Wakabayashi S., Matsubara H.;
RT   "Complete amino acid sequence of an alpha-amylase inhibitor in wheat kernel
RT   (0.19-inhibitor).";
RL   Biochim. Biophys. Acta 828:213-221(1985).
RN   [3]
RP   DISULFIDE BONDS.
RX   PubMed=6605967; DOI=10.1093/oxfordjournals.jbchem.a134429;
RA   Maeda K., Wakabayashi S., Matsubara H.;
RT   "Disulfide bridges in an alpha-amylase inhibitor from wheat kernel.";
RL   J. Biochem. 94:865-870(1983).
CC   -!- FUNCTION: Alpha-amylase inhibitor.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Endosperm.
CC   -!- PTM: The disulfide bonds are essential for the inhibitor activity.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I6 (cereal trypsin/alpha-
CC       amylase inhibitor) family. {ECO:0000305}.
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DR   PIR; A90649; WIWTA5.
DR   AlphaFoldDB; P01084; -.
DR   SMR; P01084; -.
DR   Allergome; 8186; Tri a 28.
DR   PRIDE; P01084; -.
DR   Proteomes; UP000019116; Unplaced.
DR   ExpressionAtlas; P01084; baseline.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015066; F:alpha-amylase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:InterPro.
DR   CDD; cd00261; AAI_SS; 1.
DR   Gene3D; 1.10.110.10; -; 1.
DR   InterPro; IPR044723; AAI_SS_dom.
DR   InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib.
DR   InterPro; IPR006105; Allergen/tryp_amyl_inhib_CS.
DR   InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR   InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR   Pfam; PF00234; Tryp_alpha_amyl; 1.
DR   PIRSF; PIRSF001657; Allergen/amylase_inhib; 1.
DR   PRINTS; PR00808; AMLASEINHBTR.
DR   SUPFAM; SSF47699; SSF47699; 1.
DR   PROSITE; PS00426; CEREAL_TRYP_AMYL_INH; 1.
PE   1: Evidence at protein level;
KW   Alpha-amylase inhibitor; Direct protein sequencing; Disulfide bond;
KW   Reference proteome; Secreted.
FT   CHAIN           1..124
FT                   /note="Alpha-amylase inhibitor 0.53"
FT                   /id="PRO_0000070486"
FT   DISULFID        20..41
FT                   /evidence="ECO:0000305|PubMed:6605967"
FT   DISULFID        28..83
FT                   /evidence="ECO:0000269|PubMed:6605967"
FT   DISULFID        42..99
FT                   /evidence="ECO:0000305|PubMed:6605967"
FT   DISULFID        54..115
FT                   /evidence="ECO:0000305|PubMed:6605967"
SQ   SEQUENCE   124 AA;  13185 MW;  A17C896EAB99E4B8 CRC64;
     SGPWMCYPGQ AFQVPALPGC RPLLKLQCNG SQVPEAVLRD CCQQLADISE WPRCGALYSM
     LDSMYKEHGV SEGQAGTGAF PSCRREVVKL TAASITAVCR LPIVVDASGD GAYVCKDVAA
     YPDA