IAA7_ARATH
ID IAA7_ARATH Reviewed; 243 AA.
AC Q38825; Q8LAK1;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Auxin-responsive protein IAA7;
DE AltName: Full=Auxin resistant 2;
DE AltName: Full=Indoleacetic acid-induced protein 7;
GN Name=IAA7; Synonyms=AXR2; OrderedLocusNames=At3g23050; ORFNames=MXC7.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Columbia;
RX PubMed=7658471; DOI=10.1006/jmbi.1995.0454;
RA Abel S., Nguyen M.D., Theologis A.;
RT "The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis
RT thaliana.";
RL J. Mol. Biol. 251:533-549(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP MUTANT AXR2-1.
RX PubMed=10859186; DOI=10.1104/pp.123.2.563;
RA Nagpal P., Walker L.M., Young J.C., Sonawala A., Timpte C., Estelle M.,
RA Reed J.W.;
RT "AXR2 encodes a member of the Aux/IAA protein family.";
RL Plant Physiol. 123:563-574(2000).
RN [7]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=12036262; DOI=10.1023/a:1015255030047;
RA Liscum E., Reed J.W.;
RT "Genetics of Aux/IAA and ARF action in plant growth and development.";
RL Plant Mol. Biol. 49:387-400(2002).
RN [8]
RP TRANSCRIPTIONAL REPRESSION DOMAIN.
RX PubMed=14742873; DOI=10.1105/tpc.017384;
RA Tiwari S.B., Hagen G., Guilfoyle T.J.;
RT "Aux/IAA proteins contain a potent transcriptional repression domain.";
RL Plant Cell 16:533-543(2004).
CC -!- FUNCTION: Aux/IAA proteins are short-lived transcriptional factors that
CC function as repressors of early auxin response genes at low auxin
CC concentrations. Repression is thought to result from the interaction
CC with auxin response factors (ARFs), proteins that bind to the auxin-
CC responsive promoter element (AuxRE). Formation of heterodimers with ARF
CC proteins may alter their ability to modulate early auxin response genes
CC expression. {ECO:0000269|PubMed:12036262}.
CC -!- SUBUNIT: Homodimers and heterodimers. {ECO:0000250}.
CC -!- INTERACTION:
CC Q38825; Q9LW29: AFB2; NbExp=2; IntAct=EBI-602959, EBI-617556;
CC Q38825; Q9C5W9: ARF18; NbExp=3; IntAct=EBI-602959, EBI-3946783;
CC Q38825; Q8RYC8: ARF19; NbExp=6; IntAct=EBI-602959, EBI-529887;
CC Q38825; Q94AH6: CUL1; NbExp=5; IntAct=EBI-602959, EBI-532411;
CC Q38825; Q9ZR12: GRH1; NbExp=2; IntAct=EBI-602959, EBI-617479;
CC Q38825; P49677: IAA1; NbExp=3; IntAct=EBI-602959, EBI-630505;
CC Q38825; Q38828: IAA10; NbExp=4; IntAct=EBI-602959, EBI-3946434;
CC Q38825; Q38829: IAA11; NbExp=3; IntAct=EBI-602959, EBI-2367923;
CC Q38825; Q38830: IAA12; NbExp=4; IntAct=EBI-602959, EBI-617608;
CC Q38825; Q38831: IAA13; NbExp=7; IntAct=EBI-602959, EBI-1554143;
CC Q38825; O24407: IAA16; NbExp=3; IntAct=EBI-602959, EBI-632231;
CC Q38825; O24408: IAA18; NbExp=5; IntAct=EBI-602959, EBI-2295525;
CC Q38825; O24409: IAA19; NbExp=7; IntAct=EBI-602959, EBI-632257;
CC Q38825; P49678: IAA2; NbExp=7; IntAct=EBI-602959, EBI-632343;
CC Q38825; O24410: IAA20; NbExp=4; IntAct=EBI-602959, EBI-632272;
CC Q38825; Q8LAL2: IAA26; NbExp=6; IntAct=EBI-602959, EBI-3947418;
CC Q38825; Q9XFM0: IAA28; NbExp=11; IntAct=EBI-602959, EBI-3133404;
CC Q38825; Q38822: IAA3; NbExp=6; IntAct=EBI-602959, EBI-307174;
CC Q38825; Q9M1R4: IAA30; NbExp=4; IntAct=EBI-602959, EBI-3946710;
CC Q38825; Q9C5X0: IAA34; NbExp=10; IntAct=EBI-602959, EBI-3946459;
CC Q38825; Q9C8Y3: RGL1; NbExp=3; IntAct=EBI-602959, EBI-963647;
CC Q38825; Q39255: SKP1A; NbExp=4; IntAct=EBI-602959, EBI-532357;
CC Q38825; Q570C0: TIR1; NbExp=8; IntAct=EBI-602959, EBI-307183;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q38825-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Highly expressed in stems and flowers.
CC {ECO:0000269|PubMed:7658471}.
CC -!- INDUCTION: By auxin. {ECO:0000269|PubMed:7658471}.
CC -!- DOMAIN: The N-terminal half of the protein contains two conserved
CC domains I and II. Domain I includes a slightly degenerated ERF-
CC associated amphiphilic repression (EAR) motif which seems to be
CC involved in the activity of transcriptional repression. Domain II is
CC required for the correct degradation of the protein through the SCF-
CC mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA
CC proteins and auxin response factors (ARFs) occur through their C-
CC terminal dimerization domains III and IV.
CC -!- SIMILARITY: Belongs to the Aux/IAA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM65301.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U18409; AAC49048.1; -; mRNA.
DR EMBL; AB026655; BAB02096.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76710.1; -; Genomic_DNA.
DR EMBL; AF332395; AAG48759.1; -; mRNA.
DR EMBL; AY054651; AAK96842.1; -; mRNA.
DR EMBL; AY072461; AAL66876.1; -; mRNA.
DR EMBL; AY087765; AAM65301.1; ALT_INIT; mRNA.
DR PIR; S58494; S58494.
DR RefSeq; NP_188945.1; NM_113205.3. [Q38825-1]
DR PDB; 2P1N; X-ray; 2.50 A; C/F=82-94.
DR PDB; 2P1O; X-ray; 1.90 A; C=82-94.
DR PDB; 2P1Q; X-ray; 1.91 A; C=82-94.
DR PDBsum; 2P1N; -.
DR PDBsum; 2P1O; -.
DR PDBsum; 2P1Q; -.
DR AlphaFoldDB; Q38825; -.
DR SMR; Q38825; -.
DR BioGRID; 7211; 47.
DR DIP; DIP-33345N; -.
DR ELM; Q38825; -.
DR IntAct; Q38825; 46.
DR STRING; 3702.AT3G23050.1; -.
DR PaxDb; Q38825; -.
DR PRIDE; Q38825; -.
DR ProteomicsDB; 232190; -. [Q38825-1]
DR DNASU; 821879; -.
DR EnsemblPlants; AT3G23050.1; AT3G23050.1; AT3G23050. [Q38825-1]
DR GeneID; 821879; -.
DR Gramene; AT3G23050.1; AT3G23050.1; AT3G23050. [Q38825-1]
DR KEGG; ath:AT3G23050; -.
DR Araport; AT3G23050; -.
DR TAIR; locus:2094598; AT3G23050.
DR eggNOG; ENOG502QPNB; Eukaryota.
DR HOGENOM; CLU_049393_1_5_1; -.
DR InParanoid; Q38825; -.
DR OMA; IMAQKNS; -.
DR OrthoDB; 1203639at2759; -.
DR PhylomeDB; Q38825; -.
DR EvolutionaryTrace; Q38825; -.
DR PRO; PR:Q38825; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q38825; baseline and differential.
DR Genevisible; Q38825; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009630; P:gravitropism; IMP:TAIR.
DR GO; GO:0009733; P:response to auxin; TAS:TAIR.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:TAIR.
DR GO; GO:0009414; P:response to water deprivation; IMP:TAIR.
DR GO; GO:0009611; P:response to wounding; IEP:TAIR.
DR DisProt; DP01121; -.
DR InterPro; IPR033389; AUX/IAA_dom.
DR InterPro; IPR003311; AUX_IAA.
DR InterPro; IPR000270; PB1_dom.
DR PANTHER; PTHR31734; PTHR31734; 1.
DR Pfam; PF02309; AUX_IAA; 1.
DR PROSITE; PS51745; PB1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Auxin signaling pathway; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..243
FT /note="Auxin-responsive protein IAA7"
FT /id="PRO_0000112838"
FT DOMAIN 124..224
FT /note="PB1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT MOTIF 13..17
FT /note="EAR-like (transcriptional repression)"
FT MUTAGEN 87
FT /note="P->S: In axr2-1; gain of function. Affects auxin-
FT related developmental processes. Affects
FT photomorphogenesis."
FT CONFLICT 228
FT /note="V -> I (in Ref. 5; AAM65301)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 243 AA; 26381 MW; 830E9B9EA2C2B5D0 CRC64;
MIGQLMNLKA TELCLGLPGG AEAVESPAKS AVGSKRGFSE TVDLMLNLQS NKEGSVDLKN
VSAVPKEKTT LKDPSKPPAK AQVVGWPPVR NYRKNMMTQQ KTSSGAEEAS SEKAGNFGGG
AAGAGLVKVS MDGAPYLRKV DLKMYKSYQD LSDALAKMFS SFTMGNYGAQ GMIDFMNESK
LMNLLNSSEY VPSYEDKDGD WMLVGDVPWE MFVESCKRLR IMKGSEAVGL APRAMEKYCK
NRS
