IAAC2_WHEAT
ID IAAC2_WHEAT Reviewed; 145 AA.
AC P16851;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-1992, sequence version 2.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Alpha-amylase/trypsin inhibitor CM2;
DE AltName: Full=Chloroform/methanol-soluble protein CM2;
DE Flags: Precursor;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Agathe; TISSUE=Seed;
RX PubMed=1893104; DOI=10.1007/bf00020564;
RA Gautier M.-F., Alary R., Lullien V., Joudrier P.;
RT "Nucleotide sequence of a cDNA clone encoding the wheat (Triticum durum
RT Desf.) CM2 protein.";
RL Plant Mol. Biol. 16:333-334(1991).
RN [2]
RP PROTEIN SEQUENCE OF 26-53.
RA Barber D., Sanchez-Monge R., Garcia-Olmedo F., Salcedo G., Mendez E.;
RT "Evolutionary implications of sequential homologies among members of the
RT trypsin / alpha-amylase inhibitor family (CM-proteins) in wheat and
RT barley.";
RL Biochim. Biophys. Acta 873:147-151(1986).
CC -!- FUNCTION: Alpha-amylase/trypsin inhibitor. It could be involved in
CC insect defense mechanisms.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Developing endosperm.
CC -!- MISCELLANEOUS: CM proteins would be involved in the cooking quality of
CC pasta.
CC -!- SIMILARITY: Belongs to the protease inhibitor I6 (cereal trypsin/alpha-
CC amylase inhibitor) family. {ECO:0000305}.
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DR EMBL; X55454; CAA39099.1; -; mRNA.
DR PIR; B25310; B25310.
DR AlphaFoldDB; P16851; -.
DR SMR; P16851; -.
DR Allergome; 8188; Tri a 29.
DR MEROPS; I06.004; -.
DR PRIDE; P16851; -.
DR EnsemblPlants; TraesCAD_scaffold_028654_01G000200.1; TraesCAD_scaffold_028654_01G000200.1; TraesCAD_scaffold_028654_01G000200.
DR EnsemblPlants; TraesCLE_scaffold_075039_01G000200.1; TraesCLE_scaffold_075039_01G000200.1; TraesCLE_scaffold_075039_01G000200.
DR EnsemblPlants; TraesCS7B02G072000.1; TraesCS7B02G072000.1.cds1; TraesCS7B02G072000.
DR EnsemblPlants; TraesPAR_scaffold_091200_01G000200.1; TraesPAR_scaffold_091200_01G000200.1; TraesPAR_scaffold_091200_01G000200.
DR EnsemblPlants; TraesROB_scaffold_078932_01G000200.1; TraesROB_scaffold_078932_01G000200.1; TraesROB_scaffold_078932_01G000200.
DR EnsemblPlants; TraesWEE_scaffold_119267_01G000200.1; TraesWEE_scaffold_119267_01G000200.1; TraesWEE_scaffold_119267_01G000200.
DR Gramene; TraesCAD_scaffold_028654_01G000200.1; TraesCAD_scaffold_028654_01G000200.1; TraesCAD_scaffold_028654_01G000200.
DR Gramene; TraesCLE_scaffold_075039_01G000200.1; TraesCLE_scaffold_075039_01G000200.1; TraesCLE_scaffold_075039_01G000200.
DR Gramene; TraesCS7B02G072000.1; TraesCS7B02G072000.1.cds1; TraesCS7B02G072000.
DR Gramene; TraesPAR_scaffold_091200_01G000200.1; TraesPAR_scaffold_091200_01G000200.1; TraesPAR_scaffold_091200_01G000200.
DR Gramene; TraesROB_scaffold_078932_01G000200.1; TraesROB_scaffold_078932_01G000200.1; TraesROB_scaffold_078932_01G000200.
DR Gramene; TraesWEE_scaffold_119267_01G000200.1; TraesWEE_scaffold_119267_01G000200.1; TraesWEE_scaffold_119267_01G000200.
DR Proteomes; UP000019116; Unplaced.
DR ExpressionAtlas; P16851; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0015066; F:alpha-amylase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib.
DR InterPro; IPR006105; Allergen/tryp_amyl_inhib_CS.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR PRINTS; PR00808; AMLASEINHBTR.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
DR PROSITE; PS00426; CEREAL_TRYP_AMYL_INH; 1.
PE 1: Evidence at protein level;
KW Alpha-amylase inhibitor; Direct protein sequencing; Protease inhibitor;
KW Reference proteome; Secreted; Serine protease inhibitor; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000269|Ref.2"
FT CHAIN 26..145
FT /note="Alpha-amylase/trypsin inhibitor CM2"
FT /id="PRO_0000014345"
SQ SEQUENCE 145 AA; 15460 MW; 8A6C95C1DF88940C CRC64;
MASKSSITHL LLAAVLVSVF AAAAATGPYC YPGMGLPSNP LEGCREYVAQ QTCGVGIVGS
PVSTEPGNTP RDRCCKELYD ASQHCRCEAV RYFIGRTSDP NSGVLKDLPG CPREPQRDFA
KVLVTPGHCN VMTVHNTPYC LGLDI
