IAAC3_WHEAT
ID IAAC3_WHEAT Reviewed; 168 AA.
AC P17314;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Alpha-amylase/trypsin inhibitor CM3;
DE AltName: Full=Chloroform/methanol-soluble protein CM3;
DE Flags: Precursor;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Chinese Spring; TISSUE=Endosperm;
RX PubMed=2102861; DOI=10.1007/bf00016517;
RA Garcia-Maroto F., Marana C., Mena M., Garcia-Olmedo F., Carbonero P.;
RT "Cloning of cDNA and chromosomal location of genes encoding the three types
RT of subunits of the wheat tetrameric inhibitor of insect alpha-amylase.";
RL Plant Mol. Biol. 14:845-853(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Agathe; TISSUE=Seed;
RA Gautier M.-F., Alary R., Lullien V., Joudrier P.;
RL Submitted (JUN-1991) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 26-60.
RC STRAIN=cv. Turgidum; TISSUE=Endosperm;
RA Shewry P.R., Lafiandra D., Salcedo G., Aragoncillo C., Garcia-Olmedo F.,
RA Lew E.J.-L., Dietler M.D., Kasarda D.D.;
RT "N-terminal amino acid sequence of chloroform/methanol-soluble proteins and
RT albumins from endosperm of wheat, barley and related species.";
RL FEBS Lett. 175:359-363(1984).
CC -!- FUNCTION: Alpha-amylase/trypsin inhibitor. It could be involved in
CC insect defense mechanisms.
CC -!- SUBUNIT: Subunit of the tetrameric inhibitor.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Developing endosperm.
CC -!- PTM: Five disulfide bonds are present (Probable), which are essential
CC for the inhibitor activity.
CC -!- MISCELLANEOUS: CM proteins would be involved in the cooking quality of
CC pasta.
CC -!- SIMILARITY: Belongs to the protease inhibitor I6 (cereal trypsin/alpha-
CC amylase inhibitor) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X17574; CAA35597.1; -; mRNA.
DR EMBL; X61032; CAA43367.1; -; mRNA.
DR PIR; S10029; S10029.
DR AlphaFoldDB; P17314; -.
DR SMR; P17314; -.
DR Allergome; 1051; Tri a 30.
DR Allergome; 8191; Tri a 30.0101.
DR MEROPS; I06.004; -.
DR PRIDE; P17314; -.
DR EnsemblPlants; TraesCAD_scaffold_059374_01G000200.1; TraesCAD_scaffold_059374_01G000200.1; TraesCAD_scaffold_059374_01G000200.
DR EnsemblPlants; TraesCLE_scaffold_030329_01G000100.1; TraesCLE_scaffold_030329_01G000100.1; TraesCLE_scaffold_030329_01G000100.
DR EnsemblPlants; TraesCS4B02G328100.1; TraesCS4B02G328100.1.cds1; TraesCS4B02G328100.
DR EnsemblPlants; TraesPAR_scaffold_030854_01G000500.1; TraesPAR_scaffold_030854_01G000500.1; TraesPAR_scaffold_030854_01G000500.
DR EnsemblPlants; TraesROB_scaffold_059406_01G000100.1; TraesROB_scaffold_059406_01G000100.1; TraesROB_scaffold_059406_01G000100.
DR EnsemblPlants; TraesWEE_scaffold_013736_01G000500.1; TraesWEE_scaffold_013736_01G000500.1; TraesWEE_scaffold_013736_01G000500.
DR Gramene; TraesCAD_scaffold_059374_01G000200.1; TraesCAD_scaffold_059374_01G000200.1; TraesCAD_scaffold_059374_01G000200.
DR Gramene; TraesCLE_scaffold_030329_01G000100.1; TraesCLE_scaffold_030329_01G000100.1; TraesCLE_scaffold_030329_01G000100.
DR Gramene; TraesCS4B02G328100.1; TraesCS4B02G328100.1.cds1; TraesCS4B02G328100.
DR Gramene; TraesPAR_scaffold_030854_01G000500.1; TraesPAR_scaffold_030854_01G000500.1; TraesPAR_scaffold_030854_01G000500.
DR Gramene; TraesROB_scaffold_059406_01G000100.1; TraesROB_scaffold_059406_01G000100.1; TraesROB_scaffold_059406_01G000100.
DR Gramene; TraesWEE_scaffold_013736_01G000500.1; TraesWEE_scaffold_013736_01G000500.1; TraesWEE_scaffold_013736_01G000500.
DR HOGENOM; CLU_113497_1_1_1; -.
DR Proteomes; UP000019116; Unplaced.
DR ExpressionAtlas; P17314; baseline and differential.
DR Genevisible; P17314; TA.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0015066; F:alpha-amylase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib.
DR InterPro; IPR006105; Allergen/tryp_amyl_inhib_CS.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR PRINTS; PR00808; AMLASEINHBTR.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
DR PROSITE; PS00426; CEREAL_TRYP_AMYL_INH; 1.
PE 1: Evidence at protein level;
KW Alpha-amylase inhibitor; Direct protein sequencing; Disulfide bond;
KW Protease inhibitor; Reference proteome; Secreted;
KW Serine protease inhibitor; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000269|Ref.3"
FT CHAIN 26..168
FT /note="Alpha-amylase/trypsin inhibitor CM3"
FT /id="PRO_0000014346"
SQ SEQUENCE 168 AA; 18221 MW; A950FF272E90D23C CRC64;
MACKSSCSLL LLAAVLLSVL AAASASGSCV PGVAFRTNLL PHCRDYVLQQ TCGTFTPGSK
LPEWMTSASI YSPGKPYLAK LYCCQELAEI SQQCRCEALR YFIALPVPSQ PVDPRSGNVG
ESGLIDLPGC PREMQWDFVR LLVAPGQCNL ATIHNVRYCP AVEQPLWI
