ID   IAAI_AMAHP              Reviewed;          32 AA.
AC   P80403;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Alpha-amylase inhibitor AAI;
GN   Name=AAI;
OS   Amaranthus hypochondriacus (Prince-of-Wales feather) (Amaranthus hybridus
OS   var. hypochondriacus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Amaranthaceae; Amaranthus.
OX   NCBI_TaxID=28502;
RN   [1]
RP   PROTEIN SEQUENCE, AND DISULFIDE BONDS.
RC   TISSUE=Seed;
RX   PubMed=8089137; DOI=10.1016/s0021-9258(17)31568-5;
RA   Chagolla-Lopez A., Blanco-Labra A., Patthy A., Sanchez R., Pongor S.;
RT   "A novel alpha-amylase inhibitor from amaranth (Amaranthus hypocondriacus)
RT   seeds.";
RL   J. Biol. Chem. 269:23675-23680(1994).
RN   [2]
RP   STRUCTURE BY NMR, AND REVISION OF DISULFIDE BONDS.
RC   TISSUE=Seed;
RX   PubMed=10400675; DOI=10.1074/jbc.274.29.20473;
RA   Lu S., Deng P., Liu X., Luo J., Han R., Gu X., Liang S., Wang X., Li F.,
RA   Lozanov V., Patthy A., Pongor S.;
RT   "Solution structure of the major alpha-amylase inhibitor of the crop plant
RT   amaranth.";
RL   J. Biol. Chem. 274:20473-20478(1999).
CC   -!- FUNCTION: Alpha-amylase inhibitor. It is active against alpha-amylases
CC       from Tribolium castaneum and Prostephanus truncatus larvae.
CC   -!- TISSUE SPECIFICITY: Endosperm.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
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DR   PIR; A54845; A54845.
DR   PDB; 1CLV; X-ray; 2.00 A; I=1-32.
DR   PDB; 1HTX; NMR; -; A=1-32.
DR   PDB; 1QFD; NMR; -; A=1-32.
DR   PDBsum; 1CLV; -.
DR   PDBsum; 1HTX; -.
DR   PDBsum; 1QFD; -.
DR   AlphaFoldDB; P80403; -.
DR   BMRB; P80403; -.
DR   SMR; P80403; -.
DR   MINT; P80403; -.
DR   EvolutionaryTrace; P80403; -.
DR   GO; GO:0015066; F:alpha-amylase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0004866; F:endopeptidase inhibitor activity; IEA:InterPro.
DR   InterPro; IPR011052; Proteinase_amylase_inhib_sf.
DR   SUPFAM; SSF57027; SSF57027; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Alpha-amylase inhibitor; Direct protein sequencing;
KW   Disulfide bond; Knottin.
FT   CHAIN           1..32
FT                   /note="Alpha-amylase inhibitor AAI"
FT                   /id="PRO_0000084123"
FT   DISULFID        1..18
FT                   /evidence="ECO:0000269|PubMed:8089137"
FT   DISULFID        8..23
FT                   /evidence="ECO:0000269|PubMed:8089137"
FT   DISULFID        17..31
FT                   /evidence="ECO:0000269|PubMed:8089137"
FT   STRAND          6..8
FT                   /evidence="ECO:0007829|PDB:1CLV"
FT   HELIX           10..13
FT                   /evidence="ECO:0007829|PDB:1CLV"
FT   STRAND          26..31
FT                   /evidence="ECO:0007829|PDB:1CLV"
SQ   SEQUENCE   32 AA;  3592 MW;  4B9B744B58C39BE3 CRC64;
     CIPKWNRCGP KMDGVPCCEP YTCTSDYYGN CS