IAAS_WHEAT
ID IAAS_WHEAT Reviewed; 180 AA.
AC P16347;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Endogenous alpha-amylase/subtilisin inhibitor;
DE AltName: Full=WASI;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP PROTEIN SEQUENCE.
RA Maeda K.;
RT "The complete amino-acid sequence of the endogenous alpha-amylase inhibitor
RT in wheat.";
RL Biochim. Biophys. Acta 871:250-256(1986).
CC -!- FUNCTION: Inhibitor of endogenous alpha-amylase (wheat also produces an
CC exogenous inhibitor which inactivates alpha-amylase from animal and
CC insect origin). This inhibitor can also inhibit subtilisin.
CC -!- SIMILARITY: Belongs to the protease inhibitor I3 (leguminous Kunitz-
CC type inhibitor) family. {ECO:0000305}.
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DR PIR; S38955; S38955.
DR AlphaFoldDB; P16347; -.
DR SMR; P16347; -.
DR STRING; 4565.Traes_2BL_F26D3BB29.1; -.
DR Allergome; 9593; Tri a aA_SI.
DR MEROPS; I03.004; -.
DR PRIDE; P16347; -.
DR EnsemblPlants; TraesCAD_scaffold_006131_01G000100.1; TraesCAD_scaffold_006131_01G000100.1; TraesCAD_scaffold_006131_01G000100.
DR EnsemblPlants; TraesCLE_scaffold_010427_01G000100.1; TraesCLE_scaffold_010427_01G000100.1; TraesCLE_scaffold_010427_01G000100.
DR EnsemblPlants; TraesPAR_scaffold_018593_01G000100.1; TraesPAR_scaffold_018593_01G000100.1; TraesPAR_scaffold_018593_01G000100.
DR EnsemblPlants; TraesROB_scaffold_031486_01G000100.1; TraesROB_scaffold_031486_01G000100.1; TraesROB_scaffold_031486_01G000100.
DR EnsemblPlants; TraesWEE_scaffold_007689_01G000400.1; TraesWEE_scaffold_007689_01G000400.1; TraesWEE_scaffold_007689_01G000400.
DR Gramene; TraesCAD_scaffold_006131_01G000100.1; TraesCAD_scaffold_006131_01G000100.1; TraesCAD_scaffold_006131_01G000100.
DR Gramene; TraesCLE_scaffold_010427_01G000100.1; TraesCLE_scaffold_010427_01G000100.1; TraesCLE_scaffold_010427_01G000100.
DR Gramene; TraesPAR_scaffold_018593_01G000100.1; TraesPAR_scaffold_018593_01G000100.1; TraesPAR_scaffold_018593_01G000100.
DR Gramene; TraesROB_scaffold_031486_01G000100.1; TraesROB_scaffold_031486_01G000100.1; TraesROB_scaffold_031486_01G000100.
DR Gramene; TraesWEE_scaffold_007689_01G000400.1; TraesWEE_scaffold_007689_01G000400.1; TraesWEE_scaffold_007689_01G000400.
DR eggNOG; ENOG502S0PJ; Eukaryota.
DR Proteomes; UP000019116; Unplaced.
DR ExpressionAtlas; P16347; baseline and differential.
DR GO; GO:0015066; F:alpha-amylase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR CDD; cd00178; STI; 1.
DR InterPro; IPR011065; Kunitz_inhibitor_STI-like_sf.
DR InterPro; IPR002160; Prot_inh_Kunz-lg.
DR PANTHER; PTHR33107; PTHR33107; 1.
DR Pfam; PF00197; Kunitz_legume; 1.
DR PRINTS; PR00291; KUNITZINHBTR.
DR SMART; SM00452; STI; 1.
DR SUPFAM; SSF50386; SSF50386; 1.
DR PROSITE; PS00283; SOYBEAN_KUNITZ; 1.
PE 1: Evidence at protein level;
KW Alpha-amylase inhibitor; Direct protein sequencing; Disulfide bond;
KW Protease inhibitor; Reference proteome; Serine protease inhibitor.
FT CHAIN 1..180
FT /note="Endogenous alpha-amylase/subtilisin inhibitor"
FT /id="PRO_0000083322"
FT SITE 66..67
FT /note="Reactive bond"
FT /evidence="ECO:0000250"
FT DISULFID 42..89
FT DISULFID 143..147
SQ SEQUENCE 180 AA; 19633 MW; DFF9F2A6DD47B561 CRC64;
DPPPVHDTDG NELRADANYY VLPANRAHGG GLTMAPGHGR RCPLFVSQEA DGQRDGLPVR
IAPHGGAPSD KIIRLSTDVR ISFRAYTTCV QSTEWHIDSE LVSGRRHVIT GPVRDPSPSG
RENAFRIEKY SGAEVHEYKL MACGDSCQDL GVFRDLKGGA WFLGATEPYH VVVFKKAPPA
