ID   IAA_HORVU               Reviewed;         147 AA.
AC   P16969;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Alpha-amylase/trypsin inhibitor;
DE   Flags: Precursor;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Bomi; TISSUE=Endosperm;
RX   PubMed=2460370; DOI=10.1016/0014-5793(88)80562-3;
RA   Lazaro A., Rodriguez-Palenzuela P., Marana C., Carbonero P.,
RA   Garcia-Olmedo F.;
RT   "Signal peptide homology between the sweet protein thaumatin II and
RT   unrelated cereal alpha-amylase/trypsin inhibitors.";
RL   FEBS Lett. 239:147-150(1988).
CC   -!- FUNCTION: Alpha-amylase/trypsin inhibitor.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Endosperm.
CC   -!- PTM: Five disulfide bonds are present (Probable), which are essential
CC       for the inhibitor activity.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I6 (cereal trypsin/alpha-
CC       amylase inhibitor) family. {ECO:0000305}.
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DR   EMBL; X13443; CAA31794.1; -; mRNA.
DR   PIR; S01655; S01655.
DR   AlphaFoldDB; P16969; -.
DR   SMR; P16969; -.
DR   MEROPS; I06.002; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015066; F:alpha-amylase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00261; AAI_SS; 1.
DR   Gene3D; 1.10.110.10; -; 1.
DR   InterPro; IPR044723; AAI_SS_dom.
DR   InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib.
DR   InterPro; IPR006105; Allergen/tryp_amyl_inhib_CS.
DR   InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR   InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR   Pfam; PF00234; Tryp_alpha_amyl; 1.
DR   PRINTS; PR00808; AMLASEINHBTR.
DR   SMART; SM00499; AAI; 1.
DR   SUPFAM; SSF47699; SSF47699; 1.
DR   PROSITE; PS00426; CEREAL_TRYP_AMYL_INH; 1.
PE   2: Evidence at transcript level;
KW   Alpha-amylase inhibitor; Disulfide bond; Protease inhibitor; Secreted;
KW   Serine protease inhibitor; Signal.
FT   SIGNAL          1..21
FT   CHAIN           22..147
FT                   /note="Alpha-amylase/trypsin inhibitor"
FT                   /id="PRO_0000014354"
SQ   SEQUENCE   147 AA;  15965 MW;  D9F9ABF8B26D6CA9 CRC64;
     MASDHRRFVL SGAVLLSVLA VAAATLESVK DECQLGVDFP HNPLATCHTY VIKRVCGRGP
     SRPMLVKERC CRELAAVPDH CRCEALRILM DGVRTPEGRV VEGRLGDRRD CPREEQRAFA
     ATLVTAAECN LSSVQAPGVR LVLLADG