ID   IAA_SECCE               Reviewed;          25 AA.
AC   P83048;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 42.
DE   RecName: Full=Alpha-amylase inhibitor;
DE   Flags: Fragment;
OS   Secale cereale (Rye).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Secale.
OX   NCBI_TaxID=4550 {ECO:0000305};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBUNIT, AND MASS SPECTROMETRY.
RC   STRAIN=cv. BR-1 EST 96-66061; TISSUE=Seed cotyledon;
RX   PubMed=11137459; DOI=10.1016/s1357-2725(00)00053-4;
RA   Iulek J., Franco O.L., Silva M., Slivinski C.T., Bloch C. Jr., Rigden D.J.,
RA   Grossi de Sa M.F.;
RT   "Purification, biochemical characterisation and partial primary structure
RT   of a new alpha-amylase inhibitor from Secale cereale (rye).";
RL   Int. J. Biochem. Cell Biol. 32:1195-1204(2000).
CC   -!- FUNCTION: Inhibits alpha-amylases but not trypsin. Is more effective
CC       against insect alpha-amylases than those of mammals.
CC       {ECO:0000269|PubMed:11137459}.
CC   -!- SUBUNIT: Monomer or homodimer. {ECO:0000269|PubMed:11137459}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: May exist both in a glycosylated and in an unglycosylated form.
CC       {ECO:0000303|PubMed:11137459}.
CC   -!- MASS SPECTROMETRY: Mass=13676; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:11137459};
CC   -!- SIMILARITY: Belongs to the protease inhibitor I6 (cereal trypsin/alpha-
CC       amylase inhibitor) family. {ECO:0000305}.
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DR   AlphaFoldDB; P83048; -.
DR   SMR; P83048; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015066; F:alpha-amylase inhibitor activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Alpha-amylase inhibitor; Direct protein sequencing; Glycoprotein; Secreted.
FT   CHAIN           1..>25
FT                   /note="Alpha-amylase inhibitor"
FT                   /id="PRO_0000070492"
FT   NON_TER         25
FT                   /evidence="ECO:0000303|PubMed:11137459"
SQ   SEQUENCE   25 AA;  2713 MW;  499B389C4276DC0F CRC64;
     SEDCTPWTAT PITVLAGCRD YVGEQ