ID   IACB_PESFW              Reviewed;         262 AA.
AC   A0A1J0KKC6; W3XIX0;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2017, sequence version 1.
DT   25-MAY-2022, entry version 5.
DE   RecName: Full=Iso-A82775C biosynthesis cluster protein B {ECO:0000303|PubMed:29384350};
GN   Name=iacB {ECO:0000303|PubMed:29384350}; ORFNames=PFICI_04038;
OS   Pestalotiopsis fici (strain W106-1 / CGMCC3.15140).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Xylariomycetidae; Xylariales; Sporocadaceae; Pestalotiopsis.
OX   NCBI_TaxID=1229662;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP   INDUCTION.
RC   STRAIN=W106-1 / CGMCC3.15140;
RX   PubMed=29384350; DOI=10.1021/acschembio.7b01059;
RA   Pan Y., Liu L., Guan F., Li E., Jin J., Li J., Che Y., Liu G.;
RT   "Characterization of a prenyltransferase for iso-A82775C biosynthesis and
RT   generation of new congeners of chloropestolides.";
RL   ACS Chem. Biol. 13:703-711(2018).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=W106-1 / CGMCC3.15140;
RX   PubMed=25623211; DOI=10.1186/s12864-014-1190-9;
RA   Wang X., Zhang X., Liu L., Xiang M., Wang W., Sun X., Che Y., Guo L.,
RA   Liu G., Guo L., Wang C., Yin W.B., Stadler M., Zhang X., Liu X.;
RT   "Genomic and transcriptomic analysis of the endophytic fungus
RT   Pestalotiopsis fici reveals its lifestyle and high potential for synthesis
RT   of natural products.";
RL   BMC Genomics 16:28-28(2015).
CC   -!- FUNCTION: Part of the gene cluster that mediates the biosynthesis of
CC       iso-A82775C, a enylepoxycyclohexane and biosynthetic precursor of the
CC       chloropestolide anticancer natural products (PubMed:29384350). Within
CC       the cluster, the prenyltransferase iacE prenylates siccayne to generate
CC       pestalodiol E, using dimethylallyl diphosphate (DMAPP) as cosubstrate
CC       (PubMed:29384350). The probable oxidoreductase iacF is then involved in
CC       the epoxidation of pestalodiol F to pestalodiol F, which is further
CC       converted to pestalofone A by the short-chain dehydrogenase/reductase
CC       iacG (PubMed:29384350). Iso-A82775C is subsequently generated from
CC       pestalofone A by the short-chain dehydrogenase/reductase iacC
CC       (PubMed:29384350). Iso-A82775C is further condensed with maldoxin via a
CC       Diels-Alder reaction to produce the anticancer natural products
CC       chloropestolides A to E (Probable). {ECO:0000269|PubMed:29384350,
CC       ECO:0000305|PubMed:29384350}.
CC   -!- INDUCTION: Expression is co-regulated with the other genes from the
CC       iso-A82775C biosynthesis cluster and probably controlled by the
CC       cluster-specific transcription factors iacI and iacK.
CC       {ECO:0000269|PubMed:29384350}.
CC   -!- DISRUPTION PHENOTYPE: Does not affect the production of iso-A82775C.
CC       {ECO:0000269|PubMed:29384350}.
CC   -!- BIOTECHNOLOGY: Iso-A82775C is a precursor for the biosynthesis of the
CC       anticancer natural products chloropestolides A to E via a Diesls-Alder
CC       reaction with maldoxin (PubMed:29384350). In the absence of the
CC       prenyltransferase iacE, siccayne accumulates instead of iso-A82775C and
CC       can also be condensed with maldoxin to produce chloropestolides H to K,
CC       which show also antibacterial and anticancer properties
CC       (PubMed:29384350). {ECO:0000269|PubMed:29384350}.
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DR   EMBL; KU963195; APC93981.1; -; Genomic_DNA.
DR   EMBL; KI912110; ETS86013.1; -; Genomic_DNA.
DR   RefSeq; XP_007830810.1; XM_007832619.1.
DR   AlphaFoldDB; A0A1J0KKC6; -.
DR   EnsemblFungi; ETS86013; ETS86013; PFICI_04038.
DR   GeneID; 19269051; -.
DR   KEGG; pfy:PFICI_04038; -.
DR   eggNOG; ENOG502TB2V; Eukaryota.
DR   HOGENOM; CLU_075844_0_0_1; -.
DR   OMA; FNCKVAS; -.
DR   OrthoDB; 1595208at2759; -.
DR   Proteomes; UP000030651; Unassembled WGS sequence.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..262
FT                   /note="Iso-A82775C biosynthesis cluster protein B"
FT                   /id="PRO_0000451383"
SQ   SEQUENCE   262 AA;  29687 MW;  15F279BD7BF6B061 CRC64;
     MASTWFTNIE ALSYSVVEFI PYVGTVYSFK RAQLAYQERD WPRHWQSVAN FLESAIRDVI
     LFAGVEEVAG VVILHTIAES FTDKLVELYY EHNKDEADRV RQPRIEIPQP QALDPSNELV
     VVAGRTKGAH GEKVFHGKAK GVHRFHGARF AGTIKHSKYA PSGEYIQLHI PQGLFDGARV
     TFMWKWTKDE WGTENRPEVI VGTISLYIGD DKLTWFKFTK RQGAGWRPGE GSDFNCKVAS
     LNLIIASTTV GDESLKIDLE RI