ID   IACK_PESFW              Reviewed;         670 AA.
AC   A0A1J0HSL8; W3XIY0;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2017, sequence version 1.
DT   25-MAY-2022, entry version 20.
DE   RecName: Full=C6 finger domain transcription factor iacK {ECO:0000303|PubMed:29384350};
DE   AltName: Full=Iso-A82775C biosynthesis cluster protein K {ECO:0000303|PubMed:29384350};
GN   Name=iacK {ECO:0000303|PubMed:29384350}; ORFNames=PFICI_04048;
OS   Pestalotiopsis fici (strain W106-1 / CGMCC3.15140).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Xylariomycetidae; Xylariales; Sporocadaceae; Pestalotiopsis.
OX   NCBI_TaxID=1229662;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND BIOTECHNOLOGY.
RC   STRAIN=W106-1 / CGMCC3.15140;
RX   PubMed=29384350; DOI=10.1021/acschembio.7b01059;
RA   Pan Y., Liu L., Guan F., Li E., Jin J., Li J., Che Y., Liu G.;
RT   "Characterization of a prenyltransferase for iso-A82775C biosynthesis and
RT   generation of new congeners of chloropestolides.";
RL   ACS Chem. Biol. 13:703-711(2018).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=W106-1 / CGMCC3.15140;
RX   PubMed=25623211; DOI=10.1186/s12864-014-1190-9;
RA   Wang X., Zhang X., Liu L., Xiang M., Wang W., Sun X., Che Y., Guo L.,
RA   Liu G., Guo L., Wang C., Yin W.B., Stadler M., Zhang X., Liu X.;
RT   "Genomic and transcriptomic analysis of the endophytic fungus
RT   Pestalotiopsis fici reveals its lifestyle and high potential for synthesis
RT   of natural products.";
RL   BMC Genomics 16:28-28(2015).
CC   -!- FUNCTION: Transcription factor; part of the gene cluster that mediates
CC       the biosynthesis of iso-A82775C, a enylepoxycyclohexane and
CC       biosynthetic precursor of the chloropestolide anticancer natural
CC       products. {ECO:0000269|PubMed:29384350}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- BIOTECHNOLOGY: Iso-A82775C is a precursor for the biosynthesis of the
CC       anticancer natural products chloropestolides A to E via a Diesls-Alder
CC       reaction with maldoxin (PubMed:29384350). In the absence of the
CC       prenyltransferase iacE, siccayne accumulates instead of iso-A82775C and
CC       can also be condensed with maldoxin to produce chloropestolides H to K,
CC       which show also antibacterial and anticancer properties
CC       (PubMed:29384350). {ECO:0000269|PubMed:29384350}.
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DR   EMBL; KU963195; APC57602.1; -; Genomic_DNA.
DR   EMBL; KI912110; ETS86023.1; -; Genomic_DNA.
DR   RefSeq; XP_007830820.1; XM_007832629.1.
DR   AlphaFoldDB; A0A1J0HSL8; -.
DR   STRING; 1229662.W3XIY0; -.
DR   EnsemblFungi; ETS86023; ETS86023; PFICI_04048.
DR   GeneID; 19269061; -.
DR   KEGG; pfy:PFICI_04048; -.
DR   eggNOG; ENOG502RF8N; Eukaryota.
DR   HOGENOM; CLU_015502_0_1_1; -.
DR   OMA; RTWWDLF; -.
DR   OrthoDB; 275295at2759; -.
DR   Proteomes; UP000030651; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..670
FT                   /note="C6 finger domain transcription factor iacK"
FT                   /id="PRO_0000451381"
FT   DNA_BIND        88..114
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..84
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          122..157
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        18..34
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        41..56
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        58..79
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        126..156
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   670 AA;  73313 MW;  143C5A811F366313 CRC64;
     MNTSPDYAQP GPNQFHHGAP SIYSSSSSNG VGGEVPITAQ LLRGPPPPPP PPPTATATAA
     TAAATTTTAA PSATGEPKQS GPTVPAACLA CRSKHLKCDG GNPCARCQAS ESICQYVASR
     RGYKGPRRNG TQNPNKRHAA ASDDGSPNSN GSNESCPMLL GAGVATPAAP SLSAFNPGLG
     IPETPMSTIG ATPSYSGLQI YRQPYLDANG ALVDINPRKA PQTIAERCID SFYFHFFPGH
     PSVLPKDYLL RIAEQRNIEH LLAAMRWAGS LYFEVGPTRA TFFEEAMRLM YAKDVPKDGF
     LVQAMLIVLV GLDGSCQQER ARDILSDAER IAIEIGLFQR SFAAIHGQGI PVIEESWRRT
     WWDLFVVDGM VAGVHRQTNF LLFDIVADAG LPCEEHQYLA GTIPRPLYLD DFDNDIFSGE
     EYEFSSFAYR VASIRNLGRM MRLPESVFPG DNNVDRVENF LSNWRMHLPA SKRDCLNKDM
     KLDEMMFQAW MINHACSIML HQPLSQLDSS PARDVTSCAP YQMVRSGDTF NTHTRHIVTA
     AAEISKMVTY AVPITCHTHF FTCVLTLSSI VHLSKWALWF VQNDDDLRQQ IRLNIGALNK
     LSTVWSAASR ASGQVKGVAQ EIFRSKKAAQ QNNASFWVGF TQEEIISSMA ADETIMSEIN
     TMLEPVTTSG