ID   IACR_PSEPU              Reviewed;         167 AA.
AC   B0FXI7;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=HTH-type transcriptional repressor IacR {ECO:0000305};
GN   Name=iacR {ECO:0000303|PubMed:18205812};
GN   ORFNames=E6B08_12615 {ECO:0000312|EMBL:QCI12147.1};
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1290;
RX   PubMed=18205812; DOI=10.1111/j.1574-6941.2008.00436.x;
RA   Leveau J.H.J., Gerards S.;
RT   "Discovery of a bacterial gene cluster for catabolism of the plant hormone
RT   indole 3-acetic acid.";
RL   FEMS Microbiol. Ecol. 65:238-250(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1290;
RA   Laird T.S., Leveau J.H.J.;
RT   "Genome sequence of Pseudomonas putida 1290, an auxin catabolizing
RT   strain.";
RL   Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, ACTIVITY REGULATION, AND INDUCTION.
RC   STRAIN=1290;
RX   PubMed=23881445; DOI=10.1007/s10886-013-0324-x;
RA   Scott J.C., Greenhut I.V., Leveau J.H.;
RT   "Functional characterization of the bacterial iac genes for degradation of
RT   the plant hormone indole-3-acetic acid.";
RL   J. Chem. Ecol. 39:942-951(2013).
CC   -!- FUNCTION: Probably acts as a repressor of iacA expression.
CC       {ECO:0000269|PubMed:23881445}.
CC   -!- ACTIVITY REGULATION: Exposure to indole-3-acetic acid (IAA) probably
CC       relieves the repressor activity. {ECO:0000269|PubMed:23881445}.
CC   -!- INDUCTION: Induced in the presence of IAA.
CC       {ECO:0000269|PubMed:23881445}.
CC   -!- MISCELLANEOUS: Transformation of P.putida KT2440, which cannot degrade
CC       IAA, with the iac gene cluster confers the ability to grow on IAA as a
CC       sole source of carbon and energy, but not the ability to chemotaxis
CC       towards IAA. {ECO:0000269|PubMed:23881445}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EU360594; ABY62764.1; -; Genomic_DNA.
DR   EMBL; CP039371; QCI12147.1; -; Genomic_DNA.
DR   PDB; 7KUA; X-ray; 1.89 A; A=1-167.
DR   PDBsum; 7KUA; -.
DR   SMR; B0FXI7; -.
DR   EnsemblBacteria; QCI12147; QCI12147; E6B08_12615.
DR   Proteomes; UP000298551; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000835; HTH_MarR-typ.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01047; MarR; 1.
DR   SMART; SM00347; HTH_MARR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50995; HTH_MARR_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..167
FT                   /note="HTH-type transcriptional repressor IacR"
FT                   /id="PRO_0000454115"
FT   DOMAIN          30..162
FT                   /note="HTH marR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00345"
FT   DNA_BIND        76..99
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00345"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           29..31
FT                   /evidence="ECO:0007829|PDB:7KUA"
FT   HELIX           33..53
FT                   /evidence="ECO:0007829|PDB:7KUA"
FT   TURN            54..56
FT                   /evidence="ECO:0007829|PDB:7KUA"
FT   HELIX           59..71
FT                   /evidence="ECO:0007829|PDB:7KUA"
FT   STRAND          72..75
FT                   /evidence="ECO:0007829|PDB:7KUA"
FT   HELIX           76..83
FT                   /evidence="ECO:0007829|PDB:7KUA"
FT   HELIX           87..99
FT                   /evidence="ECO:0007829|PDB:7KUA"
FT   STRAND          102..107
FT                   /evidence="ECO:0007829|PDB:7KUA"
FT   STRAND          114..119
FT                   /evidence="ECO:0007829|PDB:7KUA"
FT   HELIX           121..133
FT                   /evidence="ECO:0007829|PDB:7KUA"
FT   HELIX           135..141
FT                   /evidence="ECO:0007829|PDB:7KUA"
FT   TURN            142..144
FT                   /evidence="ECO:0007829|PDB:7KUA"
FT   HELIX           147..164
FT                   /evidence="ECO:0007829|PDB:7KUA"
SQ   SEQUENCE   167 AA;  19347 MW;  3AE3ED3FF6EFD5EF CRC64;
     MSNAKNTSAA SPARKGHSHH DPASDEFRKE DFPFYWLARV HGRYTQNMER LLKKIDLDVP
     RWRVLWILNE NGESSISEIS THAIAKLSTI TKIVYRMKED GLVDTAPSPE DGRVTQVRIT
     EVGLQNIERM QEVTRELFQR SFKGLTEAQV QRLNRMLEVV FHNLETL