IACS_PIG
ID IACS_PIG Reviewed; 97 AA.
AC P26461;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Sperm-associated acrosin inhibitor;
DE Short=AI;
DE Flags: Precursor;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Seminal vesicle;
RX PubMed=8011165; DOI=10.1089/dna.1994.13.389;
RA Kwok S.C., Dai G., McMurtry J.P.;
RT "Molecular cloning and sequence analysis of the cDNA encoding porcine
RT acrosin inhibitor.";
RL DNA Cell Biol. 13:389-394(1994).
RN [2]
RP PROTEIN SEQUENCE OF 27-97.
RC TISSUE=Semen;
RX PubMed=1551420; DOI=10.1016/0014-5793(92)80347-j;
RA Jonakova V., Calvete J.J., Mann K., Schaefer W., Schmid E.R.,
RA Toepfer-Petersen E.;
RT "The complete primary structure of three isoforms of a boar sperm-
RT associated acrosin inhibitor.";
RL FEBS Lett. 297:147-150(1992).
RN [3]
RP PROTEIN SEQUENCE OF 27-39 AND 44-52, AND CHARACTERIZATION.
RC TISSUE=Seminal plasma;
RX PubMed=8603690; DOI=10.1016/0014-5793(95)01513-2;
RA Calvete J.J., Dostalova Z., Sanz L., Adermann K., Thole H.H.,
RA Toepfer-Petersen E.;
RT "Mapping the heparin-binding domain of boar spermadhesins.";
RL FEBS Lett. 379:207-211(1996).
CC -!- FUNCTION: Inhibits acrosin.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Seminal plasma.
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DR EMBL; U09985; AAA18798.1; -; mRNA.
DR PIR; S20394; S20394.
DR RefSeq; NP_999042.1; NM_213877.1.
DR AlphaFoldDB; P26461; -.
DR BioGRID; 1149044; 2.
DR STRING; 9823.ENSSSCP00000019802; -.
DR MEROPS; I01.979; -.
DR PaxDb; P26461; -.
DR Ensembl; ENSSSCT00000080364; ENSSSCP00000062224; ENSSSCG00000029257.
DR GeneID; 396905; -.
DR KEGG; ssc:396905; -.
DR eggNOG; ENOG502SEPY; Eukaryota.
DR GeneTree; ENSGT00940000165308; -.
DR HOGENOM; CLU_169765_2_0_1; -.
DR InParanoid; P26461; -.
DR OrthoDB; 1636538at2759; -.
DR Proteomes; UP000008227; Chromosome 2.
DR Proteomes; UP000314985; Unplaced.
DR Bgee; ENSSSCG00000029257; Expressed in epididymis and 43 other tissues.
DR ExpressionAtlas; P26461; baseline and differential.
DR Genevisible; P26461; SS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR Pfam; PF00050; Kazal_1; 1.
DR SMART; SM00280; KAZAL; 1.
DR SUPFAM; SSF100895; SSF100895; 1.
DR PROSITE; PS00282; KAZAL_1; 1.
DR PROSITE; PS51465; KAZAL_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW Reference proteome; Secreted; Serine protease inhibitor; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000269|PubMed:1551420,
FT ECO:0000269|PubMed:8603690"
FT CHAIN 27..97
FT /note="Sperm-associated acrosin inhibitor"
FT /id="PRO_0000016560"
FT DOMAIN 32..90
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT SITE 50..51
FT /note="Reactive bond"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 38..70
FT DISULFID 48..67
FT DISULFID 56..88
FT VARIANT 27..28
FT /note="Missing (in one isoform)"
FT VARIANT 95..97
FT /note="Missing (in major isoform)"
SQ SEQUENCE 97 AA; 11141 MW; 2DB9CF5EE21C1171 CRC64;
MAFFSSRVRA LFILVLVLPL CSETGFARSK KTRKEPDCDV YRSHLFFCTR EMDPICGTNG
KSYANPCIFC SEKLGRNEKF DFGHWGHCRE YTSAARS
