ID   IAFRS_STRV4             Reviewed;         384 AA.
AC   G2P5T1;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   16-NOV-2011, sequence version 1.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=Isoafricanol synthase {ECO:0000303|PubMed:24626486};
DE            EC=4.2.3.157 {ECO:0000269|PubMed:24626486};
GN   ORFNames=Strvi_5748 {ECO:0000312|EMBL:AEM85259.1};
OS   Streptomyces violaceusniger (strain Tu 4113).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces violaceusniger group.
OX   NCBI_TaxID=653045;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tu 4133;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA   Peters L., Ivanova N., Daligault H., Detter J.C., Han C., Tapia R.,
RA   Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Hagen A., Katz L.,
RA   Fiedler H.-P., Keasling J., Fortman J., Woyke T.;
RT   "Complete sequence of chromosome of Streptomyces violaceusniger Tu 4113.";
RL   Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=24626486; DOI=10.1039/c4cc00177j;
RA   Riclea R., Citron C.A., Rinkel J., Dickschat J.S.;
RT   "Identification of isoafricanol and its terpene cyclase in Streptomyces
RT   violaceusniger using CLSA-NMR.";
RL   Chem. Commun. (Camb.) 50:4228-4230(2014).
CC   -!- FUNCTION: Catalyzes the cyclization of farnesyl diphosphate (FPP) to
CC       isoafricanol. {ECO:0000269|PubMed:24626486}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,6E)-farnesyl diphosphate + H2O = (+)-isoafricanol +
CC         diphosphate; Xref=Rhea:RHEA:53616, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:137522, ChEBI:CHEBI:175763;
CC         EC=4.2.3.157; Evidence={ECO:0000269|PubMed:24626486};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:B5GMG2};
CC       Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250|UniProtKB:B5GMG2};
CC   -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
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DR   EMBL; CP002994; AEM85259.1; -; Genomic_DNA.
DR   AlphaFoldDB; G2P5T1; -.
DR   SMR; G2P5T1; -.
DR   STRING; 653045.Strvi_5748; -.
DR   EnsemblBacteria; AEM85259; AEM85259; Strvi_5748.
DR   KEGG; svl:Strvi_5748; -.
DR   eggNOG; ENOG502Z881; Bacteria.
DR   HOGENOM; CLU_042538_4_0_11; -.
DR   BRENDA; 4.2.3.157; 15064.
DR   Proteomes; UP000008703; Chromosome.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR034686; Terpene_cyclase-like_2.
DR   SFLD; SFLDG01020; Terpene_Cyclase_Like_2; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
PE   1: Evidence at protein level;
KW   Lyase; Magnesium; Metal-binding.
FT   CHAIN           1..384
FT                   /note="Isoafricanol synthase"
FT                   /id="PRO_0000449806"
FT   BINDING         95
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:B5GMG2"
FT   BINDING         95
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:B5GMG2"
FT   BINDING         245
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250|UniProtKB:B5GMG2"
FT   BINDING         249
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250|UniProtKB:B5GMG2"
FT   BINDING         253
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000250|UniProtKB:B5GMG2"
SQ   SEQUENCE   384 AA;  42360 MW;  2C5878A98067A959 CRC64;
     MHAHASRPQA RQTTLLRRAA LFDFPASADL SPGTEAARHH TIQWLSRFGV FEGHESVAEY
     DALRFDVLAG LFYPRATGAD LNLGSDLVGW YFVFDDQFDG ELGSRPEAVA RLVADVIRIT
     EEDTAHGRAQ DGEGPLLESF RDLWRRISSG RPQVWRDRFR HHWLEYLHSY HREALERTGA
     LPGAGGDAPR SVEAVLALRR HSIGVQPCLD LNEPFGGYTL PPALHGGFPM ARMREATDDV
     VVFTNDIASL DKELAVGDVH NSVIVQWERA GGELEDAVRH IADLANARYR WFEETAARLP
     ALLTEAGADP GTHHAVGRYV DGMRHVMTGN LGWSVRTARY DERGTEAVSG GRQRPWAQLT
     GAEELIRAGR GAPLPPLGSG SGSR