IASPP_CAEEL
ID IASPP_CAEEL Reviewed; 769 AA.
AC Q9XVN3; E0AHC9; E0AHD0; E0AHD1;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Apoptotic enhancer 1 protein {ECO:0000303|PubMed:12524540, ECO:0000312|WormBase:F46F3.4a};
DE AltName: Full=Ce-iASPP {ECO:0000303|PubMed:12524540};
DE AltName: Full=Inhibitor member of the ASPP family;
DE Short=Protein iASPP;
GN Name=ape-1 {ECO:0000312|WormBase:F46F3.4a};
GN ORFNames=F46F3.4 {ECO:0000312|WormBase:F46F3.4a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, INTERACTION WITH P53, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=12524540; DOI=10.1038/ng1070;
RA Bergamaschi D., Samuels Y., O'Neil N.J., Trigiante G., Crook T.,
RA Hsieh J.-K., O'Connor D.J., Zhong S., Campargue I., Tomlinson M.L.,
RA Kuwabara P.E., Lu X.;
RT "iASPP oncoprotein is a key inhibitor of p53 conserved from worm to
RT human.";
RL Nat. Genet. 33:162-167(2003).
CC -!- FUNCTION: Negetively regulates apoptosis via its interaction with cep-
CC 1. {ECO:0000269|PubMed:12524540}.
CC -!- SUBUNIT: Interacts with cep-1/p53; the interaction inhibits pro-
CC apoptotic activity of cep-1. {ECO:0000269|PubMed:12524540}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:12524540}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=a {ECO:0000312|WormBase:F46F3.4a};
CC IsoId=Q9XVN3-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:F46F3.4b};
CC IsoId=Q9XVN3-2; Sequence=VSP_060342;
CC Name=c {ECO:0000312|WormBase:F46F3.4c};
CC IsoId=Q9XVN3-3; Sequence=VSP_060341;
CC Name=d {ECO:0000312|WormBase:F46F3.4d};
CC IsoId=Q9XVN3-4; Sequence=VSP_060340;
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in an increase in
CC number of apoptotic germ cells (PubMed:12524540). Double RNAi-mediated
CC knockdown together with cep-1 abrogates this increased number of
CC apoptotic germ cells (PubMed:12524540). {ECO:0000269|PubMed:12524540}.
CC -!- SIMILARITY: Belongs to the iASPP family. {ECO:0000305}.
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DR EMBL; BX284605; CAB03115.1; -; Genomic_DNA.
DR EMBL; BX284605; CBW44381.1; -; Genomic_DNA.
DR EMBL; BX284605; CBW44382.1; -; Genomic_DNA.
DR EMBL; BX284605; CBW44383.1; -; Genomic_DNA.
DR PIR; T22316; T22316.
DR RefSeq; NP_001256353.1; NM_001269424.1. [Q9XVN3-1]
DR RefSeq; NP_001256354.1; NM_001269425.1. [Q9XVN3-2]
DR RefSeq; NP_001256355.1; NM_001269426.1. [Q9XVN3-3]
DR RefSeq; NP_001256356.1; NM_001269427.1. [Q9XVN3-4]
DR AlphaFoldDB; Q9XVN3; -.
DR SMR; Q9XVN3; -.
DR BioGRID; 44626; 12.
DR IntAct; Q9XVN3; 10.
DR STRING; 6239.F46F3.4a; -.
DR iPTMnet; Q9XVN3; -.
DR EPD; Q9XVN3; -.
DR PaxDb; Q9XVN3; -.
DR EnsemblMetazoa; F46F3.4a.1; F46F3.4a.1; WBGene00000146. [Q9XVN3-1]
DR EnsemblMetazoa; F46F3.4b.1; F46F3.4b.1; WBGene00000146. [Q9XVN3-2]
DR EnsemblMetazoa; F46F3.4c.1; F46F3.4c.1; WBGene00000146. [Q9XVN3-3]
DR EnsemblMetazoa; F46F3.4d.1; F46F3.4d.1; WBGene00000146. [Q9XVN3-4]
DR GeneID; 179601; -.
DR KEGG; cel:CELE_F46F3.4; -.
DR UCSC; F46F3.4; c. elegans. [Q9XVN3-1]
DR CTD; 179601; -.
DR WormBase; F46F3.4a; CE19874; WBGene00000146; ape-1. [Q9XVN3-1]
DR WormBase; F46F3.4b; CE45256; WBGene00000146; ape-1. [Q9XVN3-2]
DR WormBase; F46F3.4c; CE45269; WBGene00000146; ape-1. [Q9XVN3-3]
DR WormBase; F46F3.4d; CE45244; WBGene00000146; ape-1. [Q9XVN3-4]
DR eggNOG; KOG0515; Eukaryota.
DR GeneTree; ENSGT00940000153463; -.
DR HOGENOM; CLU_391406_0_0_1; -.
DR InParanoid; Q9XVN3; -.
DR OMA; QKKGRTH; -.
DR OrthoDB; 564568at2759; -.
DR PRO; PR:Q9XVN3; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00000146; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; ISS:WormBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0002039; F:p53 binding; IPI:WormBase.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:WormBase.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF00018; SH3_1; 1.
DR PRINTS; PR00452; SH3DOMAIN.
DR SMART; SM00248; ANK; 2.
DR SMART; SM00326; SH3; 1.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF50044; SSF50044; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 2.
DR PROSITE; PS50002; SH3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ANK repeat; Apoptosis; Nucleus; Proto-oncogene;
KW Reference proteome; Repeat; SH3 domain.
FT CHAIN 1..769
FT /note="Apoptotic enhancer 1 protein"
FT /id="PRO_0000066968"
FT REPEAT 585..617
FT /note="ANK 1"
FT /evidence="ECO:0000255"
FT REPEAT 618..652
FT /note="ANK 2"
FT /evidence="ECO:0000255"
FT DOMAIN 684..746
FT /note="SH3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT REGION 69..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 265..396
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 451..518
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 267..285
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 322..382
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..428
FT /note="Missing (in isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_060340"
FT VAR_SEQ 1..336
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_060341"
FT VAR_SEQ 1..64
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060342"
SQ SEQUENCE 769 AA; 85436 MW; B3CBE638180762AB CRC64;
MVTTSSGGGI GYPANNGVTQ VSLIHSSDSV RTVSTAPIYR PTSSMASTMA HKSSTAPFIS
ANQRMSKPPV RVVAQPPPPH PQALSQQYHQ QNPMMMYSAP NTRPHVIPTM QVQPTMAAQI
KRNNPVNAQF QNPSEMIADY GVKPQSVEMV QRVRAVRRQV ADEETELRRL RELEHETAQL
QNKNYGRERE LNVQGSMLKE AQLELRNASM RAQSLNKHLE EMYRRRQTAA AAALVEQRKM
QQHQILLARA ANQVSTQEVI RPRASVEPFQ VNNTQQQQPS PQMMKSEEFS EKRDLNGQTG
SYDAIDGSGD HQKIPTEPSY LAPCKENQQK YSELSKMAST DPHSNHSSPS TSSQKAPTLI
TFSPPSFEQK INSSTMTRDS PFVERPTSFG DSLDESRLRS GKTDLVSLRS DSLKATKRRS
WAASEGTSMS EAEMIHRLLD EQRRGRSHFI PQLPTSQEEP SAITSETYAE EVVNSESKQV
ATSSDSTNNL ELPTEQMVLG SDTTTEEDAS SCSTRSDDGQ NLEMEVAIER RTVKGILRRP
NEKMNKGRIE FDPLALLLDA ALEGELDLVR SSASKLTDVS QANDEGITAL HNAICAGHYE
IVRFLIENDA DVNAQDSDGW TPLHCAASCN NLPMVRQLVE GGGCVLASTL SDMETPVEKC
EEDEDGYDGC LKYLSAAHNS TGSINTGKVY AAYGYEAAFE DELSFDAGDE LTVIEKDKVD
KNWWTCEKNN GEKGQVPRTY LALYPSLKYR KKLNFVMFDL PLESNNNVE
