ID   IBB1_DIOGL              Reviewed;          67 AA.
AC   P82469;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Bowman-Birk type proteinase inhibitor 1;
DE   AltName: Full=Bowman-Birk type proteinase inhibitor I;
DE   AltName: Full=DgTI;
OS   Dioclea glabra.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Macropsychanthus.
OX   NCBI_TaxID=124593;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Seed;
RX   PubMed=10441512; DOI=10.1006/bbrc.1999.1099;
RA   Bueno N.R., Fritz H., Auerswald E.A., Mentele R., Sampaio M.U.,
RA   Sampaio C.A.M., Oliva M.L.V.;
RT   "Primary structure of Dioclea glabra trypsin inhibitor, DgTI, a Bowman-Birk
RT   inhibitor.";
RL   Biochem. Biophys. Res. Commun. 261:838-843(1999).
CC   -!- FUNCTION: Inhibits trypsin but not chymotrypsin. The inhibitor consists
CC       of 2 domains and has 2 sites of interaction with trypsin.
CC   -!- SUBUNIT: Monomer. Although dimerization may occur in solution.
CC   -!- TISSUE SPECIFICITY: Seed.
CC   -!- SIMILARITY: Belongs to the Bowman-Birk serine protease inhibitor
CC       family. {ECO:0000305}.
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DR   AlphaFoldDB; P82469; -.
DR   SMR; P82469; -.
DR   MEROPS; I12.001; -.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00023; BBI; 1.
DR   Gene3D; 2.10.69.10; -; 1.
DR   InterPro; IPR035995; Bowman-Birk_prot_inh.
DR   InterPro; IPR000877; Prot_inh_BBI.
DR   Pfam; PF00228; Bowman-Birk_leg; 2.
DR   SMART; SM00269; BowB; 1.
DR   SUPFAM; SSF57247; SSF57247; 1.
DR   PROSITE; PS00281; BOWMAN_BIRK; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..67
FT                   /note="Bowman-Birk type proteinase inhibitor 1"
FT                   /id="PRO_0000105837"
FT   SITE            13..14
FT                   /note="Reactive bond for trypsin"
FT   SITE            40..41
FT                   /note="Reactive bond for trypsin"
FT   DISULFID        5..59
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        6..21
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        9..55
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        11..19
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        29..36
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        33..48
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        38..46
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
SQ   SEQUENCE   67 AA;  7242 MW;  ED7C4BCCBA9F57B1 CRC64;
     SSGPCCDRCR CTKSEPPQCQ CQDVRLNSCH SACEACVCSH SMPGLCSCLD ITHFCHEPCK
     SSGDDED