ID   IBB1_LATSA              Reviewed;          53 AA.
AC   P86701;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2012, sequence version 1.
DT   25-MAY-2022, entry version 24.
DE   RecName: Full=Bowman-Birk type proteinase inhibitor 1;
DE   AltName: Full=LSI-1 {ECO:0000303|PubMed:21647515};
DE   Flags: Fragments;
OS   Lathyrus sativus (White vetchling).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Lathyrus.
OX   NCBI_TaxID=3860;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBUNIT, AND MASS SPECTROMETRY.
RC   TISSUE=Seed {ECO:0000269|PubMed:21647515};
RX   PubMed=21647515; DOI=10.1039/c1mb05141e;
RA   Rocco M., Malorni L., Chambery A., Poerio E., Parente A., Di Maro A.;
RT   "A Bowman-Birk inhibitor with anti-elastase activity from Lathyrus sativus
RT   L. seeds.";
RL   Mol. Biosyst. 7:2500-2507(2011).
CC   -!- FUNCTION: Inhibits trypsin (IC(50)=6.20 nM), neutrophil elastase
CC       (ELANE) and, to a lesser extent, alpha-chymotrypsin (IC(50)=3.44 uM).
CC       {ECO:0000269|PubMed:21647515}.
CC   -!- SUBUNIT: Dimer. {ECO:0000269|PubMed:21647515, ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=7914.4; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:21647515};
CC   -!- SIMILARITY: Belongs to the Bowman-Birk serine protease inhibitor
CC       family. {ECO:0000255}.
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DR   AlphaFoldDB; P86701; -.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0010951; P:negative regulation of endopeptidase activity; IDA:UniProtKB.
DR   CDD; cd00023; BBI; 1.
DR   Gene3D; 2.10.69.10; -; 1.
DR   InterPro; IPR035995; Bowman-Birk_prot_inh.
DR   InterPro; IPR000877; Prot_inh_BBI.
DR   Pfam; PF00228; Bowman-Birk_leg; 2.
DR   SMART; SM00269; BowB; 1.
DR   SUPFAM; SSF57247; SSF57247; 1.
DR   PROSITE; PS00281; BOWMAN_BIRK; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..>53
FT                   /note="Bowman-Birk type proteinase inhibitor 1"
FT                   /id="PRO_0000419008"
FT   SITE            16..17
FT                   /note="Reactive bond for trypsin"
FT                   /evidence="ECO:0000250|UniProtKB:P01055"
FT   DISULFID        8..?
FT                   /evidence="ECO:0000250|UniProtKB:P01055"
FT   DISULFID        9..24
FT                   /evidence="ECO:0000250|UniProtKB:P01055"
FT   DISULFID        12..51
FT                   /evidence="ECO:0000250|UniProtKB:P01055"
FT   DISULFID        14..22
FT                   /evidence="ECO:0000250|UniProtKB:P01055"
FT   DISULFID        31..38
FT                   /evidence="ECO:0000250|UniProtKB:P01055"
FT   DISULFID        40..48
FT                   /evidence="ECO:0000250|UniProtKB:P01055"
FT   NON_CONS        49..50
FT                   /evidence="ECO:0000303|PubMed:21647515"
FT   NON_TER         53
FT                   /evidence="ECO:0000303|PubMed:21647515"
SQ   SEQUENCE   53 AA;  5751 MW;  FA7F6D8541841F0C CRC64;
     GDDVKSACCD TCLCTKSNPP ICRCVDIRET CHSACNSCVC TASIPPQCRF CYK