ID   IBB2A_LATSA             Reviewed;          72 AA.
AC   P86932;
DT   15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2017, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Bowman-Birk type proteinase inhibitor 2a {ECO:0000305|PubMed:23064155};
DE   AltName: Full=LSI-2a {ECO:0000303|PubMed:23064155};
OS   Lathyrus sativus (White vetchling).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Lathyrus.
OX   NCBI_TaxID=3860;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBUNIT, MASS SPECTROMETRY, AND IDENTIFICATION
RP   BY MASS SPECTROMETRY.
RC   TISSUE=Seed {ECO:0000303|PubMed:23064155};
RX   PubMed=23064155; DOI=10.1039/c2mb25241d;
RA   Tamburino R., Severino V., Sandomenico A., Ruvo M., Parente A.,
RA   Chambery A., Di Maro A.;
RT   "De novo sequencing and characterization of a novel Bowman-Birk inhibitor
RT   from Lathyrus sativus L. seeds by electrospray mass spectrometry.";
RL   Mol. Biosyst. 8:3232-3241(2012).
CC   -!- FUNCTION: Inhibits trypsin (IC(50)=0.9 nM) and alpha-chymotrypsin
CC       (IC(50)=1.1 nM). {ECO:0000269|PubMed:23064155}.
CC   -!- SUBUNIT: Dimer. {ECO:0000269|PubMed:23064155}.
CC   -!- MASS SPECTROMETRY: Mass=7885.9; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:23064155};
CC   -!- SIMILARITY: Belongs to the Bowman-Birk serine protease inhibitor
CC       family. {ECO:0000255}.
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DR   AlphaFoldDB; P86932; -.
DR   SMR; P86932; -.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR   GO; GO:1900004; P:negative regulation of serine-type endopeptidase activity; IDA:UniProtKB.
DR   CDD; cd00023; BBI; 1.
DR   Gene3D; 2.10.69.10; -; 1.
DR   InterPro; IPR035995; Bowman-Birk_prot_inh.
DR   InterPro; IPR000877; Prot_inh_BBI.
DR   Pfam; PF00228; Bowman-Birk_leg; 1.
DR   SMART; SM00269; BowB; 1.
DR   SUPFAM; SSF57247; SSF57247; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..72
FT                   /note="Bowman-Birk type proteinase inhibitor 2a"
FT                   /evidence="ECO:0000269|PubMed:23064155"
FT                   /id="PRO_0000438850"
FT   SITE            16..17
FT                   /note="Reactive bond for trypsin"
FT                   /evidence="ECO:0000250|UniProtKB:P56679"
FT   SITE            42..43
FT                   /note="Reactive bond for chymotrypsin"
FT                   /evidence="ECO:0000250|UniProtKB:P56679"
FT   DISULFID        8..61
FT                   /evidence="ECO:0000250|UniProtKB:P56679"
FT   DISULFID        9..24
FT                   /evidence="ECO:0000250|UniProtKB:P56679"
FT   DISULFID        12..57
FT                   /evidence="ECO:0000250|UniProtKB:P56679"
FT   DISULFID        14..22
FT                   /evidence="ECO:0000250|UniProtKB:P56679"
FT   DISULFID        31..38
FT                   /evidence="ECO:0000250|UniProtKB:P56679"
FT   DISULFID        35..50
FT                   /evidence="ECO:0000250|UniProtKB:P56679"
FT   DISULFID        40..48
FT                   /evidence="ECO:0000250|UniProtKB:P56679"
SQ   SEQUENCE   72 AA;  7899 MW;  FD1E71FABBF415E7 CRC64;
     GDDVKSACCD TCLCTKSNPP TCRCVDVGET CHSACLSCIC AYSYPPKCQC FDTQKFCYRA
     CHNSEKEEVI KG