ID   IBB4_PHIVI              Reviewed;          80 AA.
AC   P16343;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Bowman-Birk type proteinase inhibitor DE-4;
DE            Short=DE4;
OS   Philenoptera violacea (Apple-leaf) (Capassa violacea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Millettieae; Philenoptera.
OX   NCBI_TaxID=3926;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Seed;
RA   Joubert F.J.;
RT   "Proteinase inhibitors from Lonchocarpus capassa (apple-leaf) seed.";
RL   Phytochemistry 23:957-961(1984).
CC   -!- SIMILARITY: Belongs to the Bowman-Birk serine protease inhibitor
CC       family. {ECO:0000305}.
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DR   MEROPS; I12.008; -.
DR   PRIDE; P16343; -.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00023; BBI; 1.
DR   Gene3D; 2.10.69.10; -; 1.
DR   InterPro; IPR035995; Bowman-Birk_prot_inh.
DR   InterPro; IPR000877; Prot_inh_BBI.
DR   Pfam; PF00228; Bowman-Birk_leg; 1.
DR   SMART; SM00269; BowB; 1.
DR   SUPFAM; SSF57247; SSF57247; 1.
DR   PROSITE; PS00281; BOWMAN_BIRK; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..80
FT                   /note="Bowman-Birk type proteinase inhibitor DE-4"
FT                   /id="PRO_0000105843"
FT   SITE            25..26
FT                   /note="Reactive bond for trypsin"
FT                   /evidence="ECO:0000250"
FT   SITE            52..53
FT                   /note="Reactive bond for chymotrypsin"
FT                   /evidence="ECO:0000250"
FT   DISULFID        18..71
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        19..33
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        22..67
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        23..31
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        41..48
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        45..60
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        50..58
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
SQ   SEQUENCE   80 AA;  8806 MW;  6E8DF76866B871C9 CRC64;
     DDDHSDDEPR ESESSKPCCS SCCTRSRPPQ CQCTDVRLNS CHSACKSCMC TFSDPGMCSC
     LDVTDFCYKP CKSSGDDDZZ