ID   IBBD2_SOYBN             Reviewed;          83 AA.
AC   P01064;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 2.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Bowman-Birk type proteinase inhibitor D-II;
DE   AltName: Full=IV;
DE   Flags: Precursor;
OS   Glycine max (Soybean) (Glycine hispida).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC   Glycine subgen. Soja.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   AGRICOLA=IND92000914; DOI=10.1007/BF00014184;
RA   Joudrier P.E., Foard D.E., Floener L.A., Larkins B.A.;
RT   "Isolation and sequence of cDNA encoding the soybean protease inhibitors PI
RT   IV and C-II.";
RL   Plant Mol. Biol. 10:35-42(1987).
RN   [2]
RP   PROTEIN SEQUENCE OF 9-83.
RX   PubMed=641033; DOI=10.1093/oxfordjournals.jbchem.a131967;
RA   Odani S., Ikenaka T.;
RT   "Studies on soybean trypsin inhibitors, XII. Linear sequences of two
RT   soybean double-headed trypsin inhibitors, D-II and E-I.";
RL   J. Biochem. 83:737-745(1978).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 18-81, AND DISULFIDE BONDS.
RX   PubMed=1730730; DOI=10.2210/pdb1pi2/pdb;
RA   Chen P., Rose J., Love R., Wei C.H., Wang B.C.;
RT   "Reactive sites of an anticarcinogenic Bowman-Birk proteinase inhibitor are
RT   similar to other trypsin inhibitors.";
RL   J. Biol. Chem. 267:1990-1994(1992).
CC   -!- SIMILARITY: Belongs to the Bowman-Birk serine protease inhibitor
CC       family. {ECO:0000305}.
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DR   EMBL; M20733; AAA33954.1; -; mRNA.
DR   PIR; S07941; S07941.
DR   RefSeq; NP_001235006.1; NM_001248077.1.
DR   PDB; 1PI2; X-ray; 2.50 A; A=18-81.
DR   PDBsum; 1PI2; -.
DR   AlphaFoldDB; P01064; -.
DR   SMR; P01064; -.
DR   STRING; 3847.GLYMA16G33400.1; -.
DR   MEROPS; I12.001; -.
DR   MEROPS; I12.008; -.
DR   PRIDE; P01064; -.
DR   EvolutionaryTrace; P01064; -.
DR   Proteomes; UP000008827; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00023; BBI; 1.
DR   Gene3D; 2.10.69.10; -; 1.
DR   InterPro; IPR035995; Bowman-Birk_prot_inh.
DR   InterPro; IPR000877; Prot_inh_BBI.
DR   Pfam; PF00228; Bowman-Birk_leg; 2.
DR   SMART; SM00269; BowB; 1.
DR   SUPFAM; SSF57247; SSF57247; 1.
DR   PROSITE; PS00281; BOWMAN_BIRK; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond;
KW   Protease inhibitor; Reference proteome; Serine protease inhibitor.
FT   PROPEP          1..8
FT                   /evidence="ECO:0000269|PubMed:641033"
FT                   /id="PRO_0000003284"
FT   CHAIN           9..83
FT                   /note="Bowman-Birk type proteinase inhibitor D-II"
FT                   /id="PRO_0000003285"
FT   SITE            32..33
FT                   /note="Reactive bond for trypsin"
FT   SITE            59..60
FT                   /note="Reactive bond for trypsin"
FT   DISULFID        24..78
FT                   /evidence="ECO:0000269|PubMed:1730730,
FT                   ECO:0007744|PDB:1PI2"
FT   DISULFID        25..40
FT                   /evidence="ECO:0000269|PubMed:1730730,
FT                   ECO:0007744|PDB:1PI2"
FT   DISULFID        28..74
FT                   /evidence="ECO:0000269|PubMed:1730730,
FT                   ECO:0007744|PDB:1PI2"
FT   DISULFID        30..38
FT                   /evidence="ECO:0000269|PubMed:1730730,
FT                   ECO:0007744|PDB:1PI2"
FT   DISULFID        48..55
FT                   /evidence="ECO:0000269|PubMed:1730730,
FT                   ECO:0007744|PDB:1PI2"
FT   DISULFID        52..67
FT                   /evidence="ECO:0000269|PubMed:1730730,
FT                   ECO:0007744|PDB:1PI2"
FT   DISULFID        57..65
FT                   /evidence="ECO:0000269|PubMed:1730730,
FT                   ECO:0007744|PDB:1PI2"
FT   STRAND          32..35
FT                   /evidence="ECO:0007829|PDB:1PI2"
FT   STRAND          44..46
FT                   /evidence="ECO:0007829|PDB:1PI2"
FT   STRAND          53..57
FT                   /evidence="ECO:0007829|PDB:1PI2"
FT   STRAND          59..62
FT                   /evidence="ECO:0007829|PDB:1PI2"
FT   STRAND          64..67
FT                   /evidence="ECO:0007829|PDB:1PI2"
FT   STRAND          71..73
FT                   /evidence="ECO:0007829|PDB:1PI2"
SQ   SEQUENCE   83 AA;  9468 MW;  55A5F9524373C20B CRC64;
     MCILSFLKSD QSSSYDDDEY SKPCCDLCMC TRSMPPQCSC EDIRLNSCHS DCKSCMCTRS
     QPGQCRCLDT NDFCYKPCKS RDD