IBBR_ORYSJ
ID IBBR_ORYSJ Reviewed; 254 AA.
AC Q0JR25; A0A0P0UXL8; C7IWR7; P07084; P93432; Q5ZCA7; Q7XZC9; Q9AWV9; Q9M3W2;
AC Q9SAS2;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Bowman-Birk type bran trypsin inhibitor;
DE AltName: Full=OSE727A;
DE AltName: Full=Protein RBBI3-3;
DE AltName: Full=RBTI;
DE Flags: Precursor;
GN Name=RBBI3.3; OrderedLocusNames=Os01g0124401, Os01g0124400, LOC_Os01g03360;
GN ORFNames=P0037C04.19, P0044F08.1;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Embryo;
RA Masumura T., Fujioka M., Matsui Y., Kumazawa Y., Tashiro M., Morita S.,
RA Tanaka K.;
RT "Cloning, expression and localization pattern of a trypsin inhibitor gene
RT from rice.";
RL Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 7-254.
RC STRAIN=cv. Lomello; TISSUE=Embryo;
RA Chen P.-W., Chow S.-H., Chen L.-J.;
RT "Nucleotide sequence of a cDNA encoding rice Bowman-Birk proteinase
RT inhibitor.";
RL (er) Plant Gene Register PGR97-015(1997).
RN [7]
RP PROTEIN SEQUENCE OF 119-251.
RC STRAIN=cv. Nipponbare;
RX PubMed=3667571; DOI=10.1093/oxfordjournals.jbchem.a122054;
RA Tashiro M., Hashino K., Shiozaki M., Ibuki F., Maki Z.;
RT "The complete amino acid sequence of rice bran trypsin inhibitor.";
RL J. Biochem. 102:297-306(1987).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 119-251, AND DISULFIDE BONDS.
RX PubMed=16754971; DOI=10.1107/s1744309106014795;
RA Lin Y.H., Li H.T., Huang Y.C., Hsieh Y.C., Guan H.H., Liu M.Y., Chang T.,
RA Wang A.H., Chen C.J.;
RT "Purification, crystallization and preliminary X-ray crystallographic
RT analysis of rice Bowman-Birk inhibitor from Oryza sativa.";
RL Acta Crystallogr. F 62:522-524(2006).
CC -!- SIMILARITY: Belongs to the Bowman-Birk serine protease inhibitor
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB68026.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAD10378.1; Type=Miscellaneous discrepancy; Note=Chimeric cDNA. Its N-terminal part is derived from the gene LOC_Os01g03390, which coded for an RBBI2.3 protein.; Evidence={ECO:0000305};
CC Sequence=BAD52869.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB098712; BAC78439.1; -; mRNA.
DR EMBL; AP002909; BAB21173.1; -; Genomic_DNA.
DR EMBL; AP003233; BAD52869.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008207; BAH90883.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS70158.1; -; Genomic_DNA.
DR EMBL; U57640; AAD10378.1; ALT_SEQ; mRNA.
DR EMBL; U76004; AAB68026.1; ALT_INIT; mRNA.
DR PIR; A27193; TIRZBR.
DR PIR; T04161; T04161.
DR RefSeq; XP_015622026.1; XM_015766540.1.
DR PDB; 2QN5; X-ray; 3.00 A; B=119-251.
DR PDBsum; 2QN5; -.
DR AlphaFoldDB; Q0JR25; -.
DR SMR; Q0JR25; -.
DR STRING; 4530.OS01T0124401-01; -.
DR MEROPS; I12.007; -.
DR PaxDb; Q0JR25; -.
DR PRIDE; Q0JR25; -.
DR EnsemblPlants; Os01t0124401-01; Os01t0124401-01; Os01g0124401.
DR GeneID; 9271230; -.
DR Gramene; Os01t0124401-01; Os01t0124401-01; Os01g0124401.
DR KEGG; osa:9271230; -.
DR eggNOG; ENOG502R48J; Eukaryota.
DR HOGENOM; CLU_059102_0_0_1; -.
DR InParanoid; Q0JR25; -.
DR OMA; HPGPVCK; -.
DR OrthoDB; 12226at2759; -.
DR EvolutionaryTrace; Q0JR25; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q0JR25; OS.
DR GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR CDD; cd00023; BBI; 3.
DR Gene3D; 2.10.69.10; -; 3.
DR InterPro; IPR035995; Bowman-Birk_prot_inh.
DR InterPro; IPR000877; Prot_inh_BBI.
DR Pfam; PF00228; Bowman-Birk_leg; 4.
DR SMART; SM00269; BowB; 3.
DR SUPFAM; SSF57247; SSF57247; 3.
DR PROSITE; PS00281; BOWMAN_BIRK; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Disulfide bond;
KW Protease inhibitor; Reference proteome; Repeat; Serine protease inhibitor;
KW Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..118
FT /evidence="ECO:0000269|PubMed:3667571"
FT /id="PRO_0000003286"
FT CHAIN 119..251
FT /note="Bowman-Birk type bran trypsin inhibitor"
FT /id="PRO_0000003287"
FT PROPEP 252..254
FT /id="PRO_0000003288"
FT REPEAT 46..120
FT REPEAT 121..187
FT REPEAT 188..251
FT SITE 135..136
FT /note="Reactive bond for trypsin"
FT /evidence="ECO:0000250"
FT SITE 201..202
FT /note="Reactive bond for trypsin"
FT /evidence="ECO:0000250"
FT DISULFID 125..185
FT /evidence="ECO:0000269|PubMed:16754971"
FT DISULFID 126..143
FT /evidence="ECO:0000269|PubMed:16754971"
FT DISULFID 152..159
FT /evidence="ECO:0000269|PubMed:16754971"
FT DISULFID 156..172
FT /evidence="ECO:0000269|PubMed:16754971"
FT DISULFID 193..248
FT /evidence="ECO:0000269|PubMed:16754971"
FT DISULFID 194..209
FT /evidence="ECO:0000269|PubMed:16754971"
FT DISULFID 199..207
FT /evidence="ECO:0000269|PubMed:16754971"
FT DISULFID 216..223
FT /evidence="ECO:0000269|PubMed:16754971"
FT DISULFID 220..236
FT /evidence="ECO:0000269|PubMed:16754971"
FT CONFLICT 121
FT /note="R -> K (in Ref. 6; AAB68026/AAD10378)"
FT /evidence="ECO:0000305"
FT CONFLICT 130
FT /note="K -> E (in Ref. 6; AAB68026/AAD10378)"
FT /evidence="ECO:0000305"
FT CONFLICT 136..137
FT /note="PD -> TN (in Ref. 6; AAB68026/AAD10378)"
FT /evidence="ECO:0000305"
FT CONFLICT 189
FT /note="P -> T (in Ref. 6; AAB68026/AAD10378)"
FT /evidence="ECO:0000305"
FT STRAND 127..131
FT /evidence="ECO:0007829|PDB:2QN5"
FT STRAND 140..143
FT /evidence="ECO:0007829|PDB:2QN5"
FT STRAND 172..179
FT /evidence="ECO:0007829|PDB:2QN5"
FT TURN 203..205
FT /evidence="ECO:0007829|PDB:2QN5"
SQ SEQUENCE 254 AA; 27790 MW; 32630D7DE579C1E8 CRC64;
MSNTTMATST ILLFLLAGLA AAHGDGDTTI RLPSDGAKAS RPRAAKPWDC CDNIEISRLM
IYPPLYRCND EVKQCAAACK ECVEAPGGDF NGGAFVCSDW FSTVDPGPKC TAALDGLSME
RPWKCCDNIK RLPTKPDPPQ WRCNDELEPS QCTAACKSCR EAPGPFPGKL ICEDIYWGAD
PGPFCTPRPW GDCCDKAFCN KMNPPTCRCM DEVKECADAC KDCQRVESSE PPRYVCKDRF
TGHPGPVCKP RAEN
