ID   IBB_ERYVA               Reviewed;          61 AA.
AC   P81705;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Bowman-Birk type proteinase inhibitor;
DE            Short=EBI;
OS   Erythrina variegata (Indian coral tree) (Erythrina indica).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Erythrina.
OX   NCBI_TaxID=3845;
RN   [1]
RP   PROTEIN SEQUENCE, AND REACTIVE SITES.
RC   STRAIN=Var. Orientalis; TISSUE=Seed;
RX   PubMed=8056744; DOI=10.1093/oxfordjournals.jbchem.a124345;
RA   Kimura M., Kouzuma Y., Abe K., Yamasaki N.;
RT   "On a Bowman-Birk family proteinase inhibitor from Erythrina variegata
RT   seeds.";
RL   J. Biochem. 115:369-372(1994).
CC   -!- FUNCTION: Strong inhibitor of trypsin with a 1:1 stoichiometry. Weaker
CC       inhibitor of chymotrypsin.
CC   -!- SIMILARITY: Belongs to the Bowman-Birk serine protease inhibitor
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   PIR; JX0309; JX0309.
DR   AlphaFoldDB; P81705; -.
DR   SMR; P81705; -.
DR   MEROPS; I12.003; -.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00023; BBI; 1.
DR   Gene3D; 2.10.69.10; -; 1.
DR   InterPro; IPR035995; Bowman-Birk_prot_inh.
DR   InterPro; IPR000877; Prot_inh_BBI.
DR   Pfam; PF00228; Bowman-Birk_leg; 2.
DR   SMART; SM00269; BowB; 1.
DR   SUPFAM; SSF57247; SSF57247; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..61
FT                   /note="Bowman-Birk type proteinase inhibitor"
FT                   /id="PRO_0000105838"
FT   SITE            12..13
FT                   /note="Reactive bond for trypsin"
FT   SITE            38..39
FT                   /note="Reactive bond for chymotrypsin"
FT   DISULFID        4..57
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        5..20
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        8..53
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        10..18
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        27..34
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        31..46
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
FT   DISULFID        36..44
FT                   /evidence="ECO:0000250|UniProtKB:P80321"
SQ   SEQUENCE   61 AA;  6717 MW;  377EE4061D0AB0F2 CRC64;
     TSACCDKCFC TKSNPPICQC RDVGETCHSA CKFCICALSY PAQCHCLDQN TFCYDKCDSD
     S