ID   IBB_VIGUN               Reviewed;          83 AA.
AC   P17734;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   02-JUN-2021, entry version 77.
DE   RecName: Full=Bowman-Birk type seed trypsin and chymotrypsin inhibitor;
DE            Short=BTCI;
OS   Vigna unguiculata (Cowpea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Vigna.
OX   NCBI_TaxID=3917;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=cv. Serido;
RX   PubMed=3426000;
RA   Morhy L., Ventura M.M.;
RT   "The complete amino acid sequence of the Vigna unguiculata (L.) Walp. seed
RT   trypsin and chymotrypsin inhibitor.";
RL   An. Acad. Bras. Cienc. 59:71-81(1987).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 1-77, AND DISULFIDE BONDS.
RX   PubMed=17142290; DOI=10.1529/biophysj.106.090555;
RA   Barbosa J.A., Silva L.P., Teles R.C., Esteves G.F., Azevedo R.B.,
RA   Ventura M.M., de Freitas S.M.;
RT   "Crystal structure of the Bowman-Birk Inhibitor from Vigna unguiculata
RT   seeds in complex with beta-trypsin at 1.55 A resolution and its structural
RT   properties in association with proteinases.";
RL   Biophys. J. 92:1638-1650(2007).
CC   -!- SIMILARITY: Belongs to the Bowman-Birk serine protease inhibitor
CC       family. {ECO:0000305}.
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DR   PDB; 2G81; X-ray; 1.55 A; I=1-77.
DR   PDB; 3RU4; X-ray; 1.68 A; B=14-74.
DR   PDB; 6EAU; X-ray; 1.18 A; I=24-32.
DR   PDB; 6EAW; X-ray; 1.29 A; I=24-28.
DR   PDB; 6EAX; X-ray; 1.19 A; I=24-28.
DR   PDBsum; 2G81; -.
DR   PDBsum; 3RU4; -.
DR   PDBsum; 6EAU; -.
DR   PDBsum; 6EAW; -.
DR   PDBsum; 6EAX; -.
DR   SMR; P17734; -.
DR   MEROPS; I12.001; -.
DR   EvolutionaryTrace; P17734; -.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00023; BBI; 1.
DR   Gene3D; 2.10.69.10; -; 1.
DR   InterPro; IPR035995; Bowman-Birk_prot_inh.
DR   InterPro; IPR000877; Prot_inh_BBI.
DR   SMART; SM00269; BowB; 1.
DR   SUPFAM; SSF57247; SSF57247; 1.
DR   PROSITE; PS00281; BOWMAN_BIRK; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond;
KW   Protease inhibitor; Serine protease inhibitor.
FT   CHAIN           1..83
FT                   /note="Bowman-Birk type seed trypsin and chymotrypsin
FT                   inhibitor"
FT                   /id="PRO_0000105856"
FT   SITE            26..27
FT                   /note="Reactive bond for trypsin"
FT                   /evidence="ECO:0000250"
FT   SITE            53..54
FT                   /note="Reactive bond for chymotrypsin"
FT                   /evidence="ECO:0000250"
FT   DISULFID        18..72
FT                   /evidence="ECO:0000269|PubMed:17142290"
FT   DISULFID        19..34
FT                   /evidence="ECO:0000269|PubMed:17142290"
FT   DISULFID        22..68
FT                   /evidence="ECO:0000269|PubMed:17142290"
FT   DISULFID        24..32
FT                   /evidence="ECO:0000269|PubMed:17142290"
FT   DISULFID        42..49
FT                   /evidence="ECO:0000269|PubMed:17142290"
FT   DISULFID        46..61
FT                   /evidence="ECO:0000269|PubMed:17142290"
FT   DISULFID        51..59
FT                   /evidence="ECO:0000269|PubMed:17142290"
FT   STRAND          16..18
FT                   /evidence="ECO:0007829|PDB:3RU4"
FT   STRAND          25..29
FT                   /evidence="ECO:0007829|PDB:6EAU"
FT   STRAND          32..34
FT                   /evidence="ECO:0007829|PDB:3RU4"
FT   STRAND          37..41
FT                   /evidence="ECO:0007829|PDB:3RU4"
FT   STRAND          47..56
FT                   /evidence="ECO:0007829|PDB:3RU4"
FT   STRAND          58..61
FT                   /evidence="ECO:0007829|PDB:3RU4"
FT   STRAND          64..67
FT                   /evidence="ECO:0007829|PDB:3RU4"
SQ   SEQUENCE   83 AA;  9101 MW;  409C4D2D3A54B976 CRC64;
     SGHHZBSTBZ ASZSSKPCCR ZCACTKSIPP ZCRCSZVRLN SCHSACKSCA CTFSIPAZCF
     CGBIBBFCYK PCKSSHSBBB BWN