ID   IBD3_HIRME              Reviewed;          56 AA.
AC   P09865;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Bdellin B-3;
DE   Flags: Fragment;
OS   Hirudo medicinalis (Medicinal leech).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Annelida; Clitellata;
OC   Hirudinea; Hirudinida; Hirudiniformes; Hirudinidae; Hirudo.
OX   NCBI_TaxID=6421;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=3828073; DOI=10.1515/bchm3.1986.367.2.1235;
RA   Fink E., Rehm H., Gippner C., Bode W., Eulitz M., Machleidt W., Fritz H.;
RT   "The primary structure of bdellin B-3 from the leech Hirudo medicinalis.
RT   Bdellin B-3 is a compact proteinase inhibitor of a 'non-classical' Kazal
RT   type. It is present in the leech in a high molecular mass form.";
RL   Biol. Chem. Hoppe-Seyler 367:1235-1242(1986).
CC   -!- FUNCTION: Proteinase inhibitor. Blocks the activity of trypsin, plasmin
CC       and sperm acrosin.
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DR   PIR; A25356; A25356.
DR   PIR; A61417; A61417.
DR   AlphaFoldDB; P09865; -.
DR   SMR; P09865; -.
DR   MEROPS; I01.020; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR002350; Kazal_dom.
DR   InterPro; IPR036058; Kazal_dom_sf.
DR   Pfam; PF00050; Kazal_1; 1.
DR   SMART; SM00280; KAZAL; 1.
DR   SUPFAM; SSF100895; SSF100895; 1.
DR   PROSITE; PS00282; KAZAL_1; 1.
DR   PROSITE; PS51465; KAZAL_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..>56
FT                   /note="Bdellin B-3"
FT                   /id="PRO_0000073196"
FT   DOMAIN          1..42
FT                   /note="Kazal-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT   SITE            8..9
FT                   /note="Reactive bond"
FT   DISULFID        4..29
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        6..25
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        14..40
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT   NON_TER         56
SQ   SEQUENCE   56 AA;  6141 MW;  215B6BDA1E462F06 CRC64;
     DTECVCTKEL HRVCGSDGVT YDNECLATCH GASVAHDHAC EGHEEHHVDE HGEDHD