IBP2_MOUSE
ID IBP2_MOUSE Reviewed; 305 AA.
AC P47877; Q61722; Q91VK7;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 2.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Insulin-like growth factor-binding protein 2;
DE Short=IBP-2;
DE Short=IGF-binding protein 2;
DE Short=IGFBP-2;
DE Short=mIGFBP-2;
DE Flags: Precursor;
GN Name=Igfbp2; Synonyms=Igfbp-2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BALB/cJ; TISSUE=Fetal liver;
RX PubMed=7680327; DOI=10.1016/0378-1119(93)90406-s;
RA Landwehr J., Kaupmann K., Heinrich G., Schwander J.;
RT "Cloning and characterization of the gene encoding murine insulin-like
RT growth factor-binding protein-2, mIGFBP-2.";
RL Gene 124:281-286(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Liver;
RX PubMed=7529732; DOI=10.1016/0303-7207(94)90051-5;
RA Schuller A.G.P., Groffen C., van Neck J.W., Zwarthoff E.C., Drop S.L.S.;
RT "cDNA cloning and mRNA expression of the six mouse insulin-like growth
RT factor binding proteins.";
RL Mol. Cell. Endocrinol. 104:57-66(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N-3; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Inhibits IGF-mediated growth and developmental rates (By
CC similarity). IGF-binding proteins prolong the half-life of the IGFs and
CC have been shown to either inhibit or stimulate the growth promoting
CC effects of the IGFs on cell culture. They alter the interaction of IGFs
CC with their cell surface receptors. {ECO:0000250}.
CC -!- SUBUNIT: Binds IGF2 more than IGF1.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Highly expressed in adult liver, but also in
CC kidney, lung, brain, spleen, testis and ovary.
CC {ECO:0000269|PubMed:7529732}.
CC -!- DEVELOPMENTAL STAGE: Expressed embryos at day 11.5. Also expressed in
CC fetal livers with expression increasing dramatically after birth.
CC Expression decreases only slightly after postnatal day 3 and remains
CC abundant thereafter. {ECO:0000269|PubMed:7529732}.
CC -!- DOMAIN: The C-terminus is required for IGF-binding and growth
CC inhibition. {ECO:0000250}.
CC -!- PTM: O-glycosylated. {ECO:0000250}.
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DR EMBL; L05439; AAB60709.1; -; Genomic_DNA.
DR EMBL; L05436; AAB60709.1; JOINED; Genomic_DNA.
DR EMBL; L05437; AAB60709.1; JOINED; Genomic_DNA.
DR EMBL; L05438; AAB60709.1; JOINED; Genomic_DNA.
DR EMBL; X81580; CAA57270.1; -; mRNA.
DR EMBL; CH466548; EDL00293.1; -; Genomic_DNA.
DR EMBL; BC012724; AAH12724.1; -; mRNA.
DR EMBL; BC054473; AAH54473.1; -; mRNA.
DR CCDS; CCDS15036.1; -.
DR PIR; I48601; I48601.
DR PIR; JN0508; JN0508.
DR RefSeq; NP_032368.2; NM_008342.3.
DR AlphaFoldDB; P47877; -.
DR SMR; P47877; -.
DR BioGRID; 200554; 2.
DR DIP; DIP-60632N; -.
DR IntAct; P47877; 2.
DR STRING; 10090.ENSMUSP00000046610; -.
DR MEROPS; I31.953; -.
DR iPTMnet; P47877; -.
DR PhosphoSitePlus; P47877; -.
DR CPTAC; non-CPTAC-3985; -.
DR MaxQB; P47877; -.
DR PaxDb; P47877; -.
DR PeptideAtlas; P47877; -.
DR PRIDE; P47877; -.
DR ProteomicsDB; 267080; -.
DR Antibodypedia; 11351; 465 antibodies from 37 providers.
DR DNASU; 16008; -.
DR Ensembl; ENSMUST00000047328; ENSMUSP00000046610; ENSMUSG00000039323.
DR GeneID; 16008; -.
DR KEGG; mmu:16008; -.
DR UCSC; uc007bkw.2; mouse.
DR CTD; 3485; -.
DR MGI; MGI:96437; Igfbp2.
DR VEuPathDB; HostDB:ENSMUSG00000039323; -.
DR eggNOG; ENOG502QRWQ; Eukaryota.
DR GeneTree; ENSGT00940000158542; -.
DR HOGENOM; CLU_070833_3_0_1; -.
DR InParanoid; P47877; -.
DR OMA; AEPCAEM; -.
DR OrthoDB; 979270at2759; -.
DR PhylomeDB; P47877; -.
DR TreeFam; TF331211; -.
DR Reactome; R-MMU-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
DR BioGRID-ORCS; 16008; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Igfbp2; mouse.
DR PRO; PR:P47877; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P47877; protein.
DR Bgee; ENSMUSG00000039323; Expressed in choroid plexus of fourth ventricle and 279 other tissues.
DR ExpressionAtlas; P47877; baseline and differential.
DR Genevisible; P47877; MM.
DR GO; GO:0016324; C:apical plasma membrane; ISO:MGI.
DR GO; GO:0031410; C:cytoplasmic vesicle; ISO:MGI.
DR GO; GO:0005576; C:extracellular region; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0005520; F:insulin-like growth factor binding; IPI:MGI.
DR GO; GO:0031994; F:insulin-like growth factor I binding; ISS:UniProtKB.
DR GO; GO:0031995; F:insulin-like growth factor II binding; ISS:UniProtKB.
DR GO; GO:0007568; P:aging; IEA:Ensembl.
DR GO; GO:0032870; P:cellular response to hormone stimulus; IEA:Ensembl.
DR GO; GO:0007565; P:female pregnancy; IEA:Ensembl.
DR GO; GO:0042104; P:positive regulation of activated T cell proliferation; ISS:UniProtKB.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0043567; P:regulation of insulin-like growth factor receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0032355; P:response to estradiol; IEA:Ensembl.
DR GO; GO:0043627; P:response to estrogen; IEA:Ensembl.
DR GO; GO:0051384; P:response to glucocorticoid; IEA:Ensembl.
DR GO; GO:0010226; P:response to lithium ion; IEA:Ensembl.
DR GO; GO:0009612; P:response to mechanical stimulus; IEA:Ensembl.
DR GO; GO:0007584; P:response to nutrient; IEA:Ensembl.
DR GO; GO:0032526; P:response to retinoic acid; IEA:Ensembl.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IEA:Ensembl.
DR GO; GO:0007165; P:signal transduction; IEA:Ensembl.
DR CDD; cd00191; TY; 1.
DR Gene3D; 4.10.800.10; -; 1.
DR InterPro; IPR009030; Growth_fac_rcpt_cys_sf.
DR InterPro; IPR012210; IGFBP-2.
DR InterPro; IPR000867; IGFBP-like.
DR InterPro; IPR022321; IGFBP_1-6_chordata.
DR InterPro; IPR017891; Insulin_GF-bd_Cys-rich_CS.
DR InterPro; IPR000716; Thyroglobulin_1.
DR InterPro; IPR036857; Thyroglobulin_1_sf.
DR Pfam; PF00219; IGFBP; 1.
DR Pfam; PF00086; Thyroglobulin_1; 1.
DR PRINTS; PR01976; IGFBPFAMILY.
DR PRINTS; PR01978; IGFBPFAMILY2.
DR SMART; SM00121; IB; 1.
DR SMART; SM00211; TY; 1.
DR SUPFAM; SSF57184; SSF57184; 1.
DR SUPFAM; SSF57610; SSF57610; 1.
DR PROSITE; PS00222; IGFBP_N_1; 1.
DR PROSITE; PS51323; IGFBP_N_2; 1.
DR PROSITE; PS00484; THYROGLOBULIN_1_1; 1.
DR PROSITE; PS51162; THYROGLOBULIN_1_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Growth factor binding; Growth regulation;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..34
FT /evidence="ECO:0000250"
FT CHAIN 35..305
FT /note="Insulin-like growth factor-binding protein 2"
FT /id="PRO_0000014371"
FT DOMAIN 36..118
FT /note="IGFBP N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00653"
FT DOMAIN 204..286
FT /note="Thyroglobulin type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00500"
FT MOTIF 281..283
FT /note="Cell attachment site"
FT DISULFID 207..241
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00500"
FT DISULFID 252..263
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00500"
FT DISULFID 265..286
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00500"
FT CONFLICT 47
FT /note="R -> A (in Ref. 2; CAA57270)"
FT /evidence="ECO:0000305"
FT CONFLICT 133
FT /note="D -> H (in Ref. 1; AAB60709)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 305 AA; 32847 MW; 179FE6A304E93A16 CRC64;
MLPRLGGPAL PLLLPSLLLL LLLGAGGCGP GVRAEVLFRC PPCTPERLAA CGPPPDAPCA
ELVREPGCGC CSVCARQEGE ACGVYIPRCA QTLRCYPNPG SELPLKALVT GAGTCEKRRV
GTTPQQVADS DDDHSEGGLV ENHVDGTMNM LGGGSSAGRK PLKSGMKELA VFREKVNEQH
RQMGKGAKHL SLEEPKKLRP PPARTPCQQE LDQVLERIST MRLPDDRGPL EHLYSLHIPN
CDKHGRYNLK QCKMSLNGQR GECWCVNPNT GKPIQGAPTI RGDPECHLFY NEQQETGGAH
AQSVQ