ICAA_STAAW
ID ICAA_STAAW Reviewed; 412 AA.
AC Q8NUI7;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Poly-beta-1,6-N-acetyl-D-glucosamine synthase;
DE Short=PNAG synthase;
DE Short=Poly-beta-1,6-GlcNAc synthase;
DE EC=2.4.1.-;
DE AltName: Full=Biofilm polysaccharide intercellular adhesin synthesis protein IcaA;
DE Short=Biofilm PIA synthesis protein IcaA;
DE AltName: Full=Intercellular adhesion protein A;
DE AltName: Full=N-acetylglucosaminyltransferase IcaA;
GN Name=icaA; OrderedLocusNames=MW2586;
OS Staphylococcus aureus (strain MW2).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=196620;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MW2;
RX PubMed=12044378; DOI=10.1016/s0140-6736(02)08713-5;
RA Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A., Nagai Y.,
RA Iwama N., Asano K., Naimi T., Kuroda H., Cui L., Yamamoto K., Hiramatsu K.;
RT "Genome and virulence determinants of high virulence community-acquired
RT MRSA.";
RL Lancet 359:1819-1827(2002).
CC -!- FUNCTION: N-acetylglucosaminyltransferase that catalyzes the
CC polymerization of single monomer units of UDP-N-acetylglucosamine to
CC produce the linear homomer poly-beta-1,6-N-acetyl-D-glucosamine (PNAG,
CC also referred to as PIA), a biofilm adhesin polysaccharide. Requires
CC IcaD for full activity (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR EMBL; BA000033; BAB96451.1; -; Genomic_DNA.
DR RefSeq; WP_001159427.1; NC_003923.1.
DR AlphaFoldDB; Q8NUI7; -.
DR SMR; Q8NUI7; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR EnsemblBacteria; BAB96451; BAB96451; BAB96451.
DR KEGG; sam:MW2586; -.
DR HOGENOM; CLU_023978_0_1_9; -.
DR OMA; RNRWAEG; -.
DR Proteomes; UP000000418; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008375; F:acetylglucosaminyltransferase activity; IEA:InterPro.
DR GO; GO:0043708; P:cell adhesion involved in biofilm formation; IEA:InterPro.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR001173; Glyco_trans_2-like.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR InterPro; IPR023853; PGA_PgaC/IcaA.
DR Pfam; PF00535; Glycos_transf_2; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
DR TIGRFAMs; TIGR03937; PgaC_IcaA; 1.
PE 3: Inferred from homology;
KW Cell membrane; Glycosyltransferase; Membrane; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..412
FT /note="Poly-beta-1,6-N-acetyl-D-glucosamine synthase"
FT /id="PRO_0000059281"
FT TRANSMEM 6..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..312
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..388
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 412 AA; 47756 MW; A57CCB45E34FE3E2 CRC64;
MQFFNFLLFY PVFMSIYWIV GSIYFYFTRE IRYSLNKKPD INVDELEGIT FLLACYNESD
TIEDTLSNVL ALKYEKKEII IINDGSSDNT AELIYKIKEN NDFIFVDLQE NRGKANALNQ
GIKQASYDYV MCLDADTIVD QDAPYYMIEN FKHDPKLGAV TGNPRIRNKS SILGKIQTIE
YASLIGCIKR SQTLAGAVNT ISGVFTLFKK SAVVDVGYWD TDMITEDIAV SWKLHLRGYR
IKYEPLAMCW MLVPETLGGL WKQRVRWAQG GHEVLLRDFF STMKTKRFPL YILMFEQIIS
ILWVYIVLLY LGYLFITANF LDYTFMTYSF SIFLLSSFTM TFINVIQFTV ALFIDSRYEK
KNMAGLIFVS WYPTVYWIIN AAVVLVAFPK ALKRKKGGYA TWSSPDRGNT QR