ICAA_STAEP
ID ICAA_STAEP Reviewed; 412 AA.
AC Q8GLC5; Q6TYB3;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 73.
DE RecName: Full=Poly-beta-1,6-N-acetyl-D-glucosamine synthase;
DE Short=PNAG synthase;
DE Short=Poly-beta-1,6-GlcNAc synthase;
DE EC=2.4.1.-;
DE AltName: Full=Biofilm polysaccharide intercellular adhesin synthesis protein IcaA;
DE Short=Biofilm PIA synthesis protein IcaA;
DE AltName: Full=Intercellular adhesion protein A;
DE AltName: Full=N-acetylglucosaminyltransferase IcaA;
GN Name=icaA;
OS Staphylococcus epidermidis.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=1282;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=97-337;
RA Li H., Wen Y.;
RL Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=SE5;
RX PubMed=15096544; DOI=10.1099/jmm.0.05372-0;
RA Handke L.D., Conlon K.M., Slater S.R., Elbaruni S., Fitzpatrick F.,
RA Humphreys H., Giles W.P., Rupp M.E., Fey P.D., O'Gara J.P.;
RT "Genetic and phenotypic analysis of biofilm phenotypic variation in
RT multiple Staphylococcus epidermidis isolates.";
RL J. Med. Microbiol. 53:367-374(2004).
CC -!- FUNCTION: N-acetylglucosaminyltransferase that catalyzes the
CC polymerization of single monomer units of UDP-N-acetylglucosamine to
CC produce the linear homomer poly-beta-1,6-N-acetyl-D-glucosamine (PNAG,
CC also referred to as PIA), a biofilm adhesin polysaccharide. Requires
CC IcaD for full activity (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR EMBL; AY382582; AAQ88120.1; -; Genomic_DNA.
DR EMBL; AY138959; AAN17771.1; -; Genomic_DNA.
DR RefSeq; WP_002484500.1; NZ_WBRV01000008.1.
DR AlphaFoldDB; Q8GLC5; -.
DR SMR; Q8GLC5; -.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR PATRIC; fig|1282.1161.peg.589; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008375; F:acetylglucosaminyltransferase activity; IEA:InterPro.
DR GO; GO:0043708; P:cell adhesion involved in biofilm formation; IEA:InterPro.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR001173; Glyco_trans_2-like.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR InterPro; IPR023853; PGA_PgaC/IcaA.
DR Pfam; PF00535; Glycos_transf_2; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
DR TIGRFAMs; TIGR03937; PgaC_IcaA; 1.
PE 3: Inferred from homology;
KW Cell membrane; Glycosyltransferase; Membrane; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..412
FT /note="Poly-beta-1,6-N-acetyl-D-glucosamine synthase"
FT /id="PRO_0000059282"
FT TRANSMEM 7..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 364..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VARIANT 106
FT /note="V -> A (in strain: 97-337)"
SQ SEQUENCE 412 AA; 47794 MW; 70B1CDA05F48B933 CRC64;
MHIFNFLLFY PIFMSIYWIV GSIYYFFIKE KPFNRLLLVK SEHQQVEGIS FLLACYNESE
TVQDTLSSVL SLEYPEKEII IINDGSSDNT AEIIYEFKKN HDFKFVDLEV NRGKANALNE
GIKQASYEYV MCLDADTVID DDAPFYMIED FKKNPKLGAV TGNPRIRNKS SILGKIQTIE
YASIIGCIKR SQSLAGAINT ISGVFTLFKK SALKDVGYWD TDMITEDIAV SWKLHLFDYE
IKYEPRALCW MLVPETIGGL WKQRVRWAQG GHEVLLRDFW STIKTKKLSL YILMFEQIAS
ITWVYIVICY LSFLVITANI LDYTYLKYSF SIFFFSSFTM TFINIIQFTV ALFIDSRYEK
KNIVGLIFLS WYPTLYWVIN AAVVIMAFPK ALKRKKGGYA TWSSPDRGNI QR
