ICAB_STAA8
ID ICAB_STAA8 Reviewed; 290 AA.
AC Q9RQP7; Q2FUU7;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase;
DE Short=PNAG N-deacetylase;
DE Short=Poly-beta-1,6-GlcNAc N-deacetylase;
DE EC=3.5.1.-;
DE AltName: Full=Biofilm polysaccharide intercellular adhesin deacetylase;
DE Short=Biofilm PIA deacetylase;
DE AltName: Full=Intercellular adhesion protein B;
DE Flags: Precursor;
GN Name=icaB; OrderedLocusNames=SAOUHSC_03004;
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], ROLE IN BIOFILM FORMATION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=10496925; DOI=10.1128/iai.67.10.5427-5433.1999;
RA Cramton S.E., Gerke C., Schnell N.F., Nichols W.W., Goetz F.;
RT "The intercellular adhesion (ica) locus is present in Staphylococcus aureus
RT and is required for biofilm formation.";
RL Infect. Immun. 67:5427-5433(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
CC -!- FUNCTION: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl-D-
CC glucosamine (PNAG, also referred to as PIA), a biofilm adhesin
CC polysaccharide. N-deacetylation is crucial for attachment of the
CC polysaccharide to the bacterial cell surface; it leads to the
CC introduction of positive charges in the otherwise neutral PIA polymer,
CC allowing electrostatic interactions (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall. Note=Attached to the cell
CC surface. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the icaADBCR genes leads to the
CC inability to form biofilms, produce PIA or mediate N-
CC acetylglucosaminyltransferase activity in vitro.
CC {ECO:0000269|PubMed:10496925}.
CC -!- SIMILARITY: Belongs to the polysaccharide deacetylase family.
CC {ECO:0000305}.
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DR EMBL; AF086783; AAD52057.1; -; Genomic_DNA.
DR EMBL; CP000253; ABD31991.1; -; Genomic_DNA.
DR RefSeq; WP_000877317.1; NZ_LS483365.1.
DR RefSeq; YP_501453.1; NC_007795.1.
DR AlphaFoldDB; Q9RQP7; -.
DR SMR; Q9RQP7; -.
DR STRING; 1280.SAXN108_2940; -.
DR EnsemblBacteria; ABD31991; ABD31991; SAOUHSC_03004.
DR GeneID; 3921486; -.
DR KEGG; sao:SAOUHSC_03004; -.
DR PATRIC; fig|93061.5.peg.2711; -.
DR eggNOG; COG0726; Bacteria.
DR HOGENOM; CLU_030024_3_2_9; -.
DR OMA; TYWHPNF; -.
DR PRO; PR:Q9RQP7; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR InterPro; IPR002509; NODB_dom.
DR InterPro; IPR023872; PNAG_deacetylase.
DR Pfam; PF01522; Polysacc_deac_1; 1.
DR SUPFAM; SSF88713; SSF88713; 1.
DR TIGRFAMs; TIGR03933; PIA_icaB; 1.
DR PROSITE; PS51677; NODB; 1.
PE 3: Inferred from homology;
KW Cell wall; Hydrolase; Reference proteome; Secreted; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..290
FT /note="Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase"
FT /id="PRO_0000024833"
FT DOMAIN 114..290
FT /note="NodB homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01014"
FT CONFLICT 116
FT /note="V -> E (in Ref. 1; AAD52057)"
FT /evidence="ECO:0000305"
FT CONFLICT 118
FT /note="I -> V (in Ref. 1; AAD52057)"
FT /evidence="ECO:0000305"
FT CONFLICT 153
FT /note="E -> G (in Ref. 1; AAD52057)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 290 AA; 34105 MW; 76D1B0DA47C726A8 CRC64;
MKYRKFIILV LSILIILPVS TLDGHHIANA DDDSPKKLKY KENSALALNY HRVRKANFLN
NFIYFFSSSK EIKNYSVSQS QFESQIKWLK SHDAKFLTLK EFLYYKKKGK FPKRSVWINF
DDMDETIYEN AYPILKKYKI PATGFIITGH VGEENFHNLD MISKKELKEM YKTGLWEFET
HTHDLHNLSK NNKSKLMKAS EATIIKDLNK SEKYLTKNFK KSQKTIAYPY GLMNDDKLPV
IKKAGLKYGF SLEEKAVTPN SNDYYIPRIL ISDDAFEHLI KRWDGFHEKD
