ID   ICAC_STAA8              Reviewed;         350 AA.
AC   Q9RQP6; Q2FUU6;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Probable poly-beta-1,6-N-acetyl-D-glucosamine export protein;
DE            Short=PGA export protein;
DE            Short=Poly-beta-1,6-GlcNAc export protein;
DE   AltName: Full=Biofilm polysaccharide intercellular adhesin export protein;
DE            Short=Biofilm PIA export protein;
DE   AltName: Full=Intercellular adhesion protein C;
GN   Name=icaC; OrderedLocusNames=SAOUHSC_03005;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], ROLE IN BIOFILM FORMATION, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=10496925; DOI=10.1128/iai.67.10.5427-5433.1999;
RA   Cramton S.E., Gerke C., Schnell N.F., Nichols W.W., Goetz F.;
RT   "The intercellular adhesion (ica) locus is present in Staphylococcus aureus
RT   and is required for biofilm formation.";
RL   Infect. Immun. 67:5427-5433(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
CC   -!- FUNCTION: Presumably involved in the export of the biofilm adhesin
CC       polysaccharide poly-beta-1,6-N-acetyl-D-glucosamine (PNAG, also
CC       referred to as PIA) across the cell membrane. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the icaADBCR genes leads to the
CC       inability to form biofilms, produce PIA or mediate N-
CC       acetylglucosaminyltransferase activity in vitro.
CC       {ECO:0000269|PubMed:10496925}.
CC   -!- SIMILARITY: Belongs to the acyltransferase 3 family. {ECO:0000305}.
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DR   EMBL; AF086783; AAD52058.1; -; Genomic_DNA.
DR   EMBL; CP000253; ABD31992.1; -; Genomic_DNA.
DR   PIR; E90075; E90075.
DR   RefSeq; WP_000723836.1; NZ_LS483365.1.
DR   RefSeq; YP_501454.1; NC_007795.1.
DR   AlphaFoldDB; Q9RQP6; -.
DR   STRING; 1280.SAXN108_2941; -.
DR   EnsemblBacteria; ABD31992; ABD31992; SAOUHSC_03005.
DR   GeneID; 3921487; -.
DR   KEGG; sao:SAOUHSC_03005; -.
DR   PATRIC; fig|93061.5.peg.2712; -.
DR   eggNOG; COG3936; Bacteria.
DR   HOGENOM; CLU_064947_1_0_9; -.
DR   OMA; YHFYFVP; -.
DR   PRO; PR:Q9RQP6; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:InterPro.
DR   InterPro; IPR002656; Acyl_transf_3_dom.
DR   Pfam; PF01757; Acyl_transf_3; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..350
FT                   /note="Probable poly-beta-1,6-N-acetyl-D-glucosamine export
FT                   protein"
FT                   /id="PRO_0000208076"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        40..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        83..103
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        119..139
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        182..202
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        216..236
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        254..274
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        276..296
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        308..328
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   350 AA;  41344 MW;  CA4BB4114B3C0596 CRC64;
     MKKIRLELVY LRAIICAIII ITHLLTQITL KHENMEGGSL VLQFYIRNIV IFGTPCFIIL
     SQLLTTLNYQ KVTYRYLTTR VKYILIPYIL MGLFYSYSES LLTDSSFNKQ FIENVLLGQW
     YGYFIVVIMQ FFILSYIIFK INYNLFNSKI LLLLSFILQQ SFLYYFTNNT AFHDTVLHYY
     PLSENTIIFG WIFYFFLGAY MGYNYERVLN FLERYLVIMI VLAVATYFVF IALANGDYWN
     VTSFSYSLTP YNSIMFIVIL GICTHFKTML FNTIQMISAF SFFIYLLHPI ILDSLFAYTN
     IFEDNTMVFL AISLLFILGL CIGVGMILRE FYIFRFIIGK QPYKLNINAY