ID   ICAD_STAA8              Reviewed;         101 AA.
AC   Q9RQP8; Q2FUU8;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Poly-beta-1,6-N-acetyl-D-glucosamine synthesis protein IcaD;
DE            Short=PGA synthesis protein IcaD;
DE            Short=Poly-beta-1,6-GlcNAc synthesis protein IcaD;
DE   AltName: Full=Biofilm polysaccharide intercellular adhesin synthesis protein IcaD;
DE            Short=Biofilm PIA synthesis protein IcaD;
DE   AltName: Full=Intercellular adhesion protein D;
GN   Name=icaD; OrderedLocusNames=SAOUHSC_03003;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], ROLE IN BIOFILM FORMATION, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=10496925; DOI=10.1128/iai.67.10.5427-5433.1999;
RA   Cramton S.E., Gerke C., Schnell N.F., Nichols W.W., Goetz F.;
RT   "The intercellular adhesion (ica) locus is present in Staphylococcus aureus
RT   and is required for biofilm formation.";
RL   Infect. Immun. 67:5427-5433(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
CC   -!- FUNCTION: Necessary for the synthesis of poly-beta-1,6-N-acetyl-D-
CC       glucosamine (PNAG, also referred to as PIA), a biofilm adhesin
CC       polysaccharide. Is required for full IcaA N-
CC       acetylglucosaminyltransferase activity (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the icaADBCR genes leads to the
CC       inability to form biofilms, produce PIA or mediate N-
CC       acetylglucosaminyltransferase activity in vitro.
CC       {ECO:0000269|PubMed:10496925}.
CC   -!- SIMILARITY: Belongs to the IcaD family. {ECO:0000305}.
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DR   EMBL; AF086783; AAD52056.1; -; Genomic_DNA.
DR   EMBL; CP000253; ABD31990.1; -; Genomic_DNA.
DR   PIR; C90075; C90075.
DR   RefSeq; WP_000240580.1; NZ_LS483365.1.
DR   RefSeq; YP_501452.1; NC_007795.1.
DR   AlphaFoldDB; Q9RQP8; -.
DR   STRING; 93061.SAOUHSC_03003; -.
DR   EnsemblBacteria; ABD31990; ABD31990; SAOUHSC_03003.
DR   GeneID; 3921485; -.
DR   KEGG; sao:SAOUHSC_03003; -.
DR   PATRIC; fig|93061.5.peg.2710; -.
DR   HOGENOM; CLU_2289916_0_0_9; -.
DR   OMA; SVITIRI; -.
DR   PRO; PR:Q9RQP8; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR020510; IcaD.
DR   TIGRFAMs; TIGR03932; PIA_icaD; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..101
FT                   /note="Poly-beta-1,6-N-acetyl-D-glucosamine synthesis
FT                   protein IcaD"
FT                   /id="PRO_0000084139"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        72..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   101 AA;  11783 MW;  3BE3E8E0CCF10F04 CRC64;
     MVKPRQREYP TLKSSLNIVR ETALIAISCV FWIYCLVVLL VYIGTIFEIH DESINTIRVA
     LNIENTEILD IFETMGIFAI IIFVFFTISI LIQKWQRGRE S