ID   ICAD_STAEQ              Reviewed;         101 AA.
AC   Q5HKP9; O54214;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Poly-beta-1,6-N-acetyl-D-glucosamine synthesis protein IcaD;
DE            Short=PGA synthesis protein IcaD;
DE            Short=Poly-beta-1,6-GlcNAc synthesis protein IcaD;
DE   AltName: Full=Biofilm polysaccharide intercellular adhesin synthesis protein IcaD;
DE            Short=Biofilm PIA synthesis protein IcaD;
DE   AltName: Full=Intercellular adhesion protein D;
GN   Name=icaD; OrderedLocusNames=SERP2294;
OS   Staphylococcus epidermidis (strain ATCC 35984 / RP62A).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176279;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8809760; DOI=10.1111/j.1365-2958.1996.tb02548.x;
RA   Heilmann C., Schweitzer O., Gerke C., Vanittanakom N., Mack D., Goetz F.;
RT   "Molecular basis of intercellular adhesion in the biofilm-forming
RT   Staphylococcus epidermidis.";
RL   Mol. Microbiol. 20:1083-1091(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35984 / RP62A;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
RN   [3]
RP   FUNCTION IN PIA SYNTHESIS, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX   PubMed=9660830; DOI=10.1074/jbc.273.29.18586;
RA   Gerke C., Kraft A., Sussmuth R., Schweitzer O., Goetz F.;
RT   "Characterization of the N-acetylglucosaminyltransferase activity involved
RT   in the biosynthesis of the Staphylococcus epidermidis polysaccharide
RT   intercellular adhesin.";
RL   J. Biol. Chem. 273:18586-18593(1998).
CC   -!- FUNCTION: Necessary for the synthesis of poly-beta-1,6-N-acetyl-D-
CC       glucosamine (PNAG, also referred to as PIA), a biofilm adhesin
CC       polysaccharide. Is required for full IcaA N-
CC       acetylglucosaminyltransferase activity. {ECO:0000269|PubMed:9660830}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:9660830};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:9660830}.
CC   -!- DISRUPTION PHENOTYPE: Loss of cell aggregation and PIA production.
CC       {ECO:0000269|PubMed:9660830}.
CC   -!- SIMILARITY: Belongs to the IcaD family. {ECO:0000305}.
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DR   EMBL; U43366; AAC06120.1; -; Genomic_DNA.
DR   EMBL; CP000029; AAW53183.1; -; Genomic_DNA.
DR   RefSeq; WP_002477257.1; NC_002976.3.
DR   AlphaFoldDB; Q5HKP9; -.
DR   SMR; Q5HKP9; -.
DR   STRING; 176279.SERP2294; -.
DR   EnsemblBacteria; AAW53183; AAW53183; SERP2294.
DR   KEGG; ser:SERP2294; -.
DR   eggNOG; ENOG50305E9; Bacteria.
DR   HOGENOM; CLU_2289916_0_0_9; -.
DR   OMA; SVITIRI; -.
DR   OrthoDB; 1909781at2; -.
DR   Proteomes; UP000000531; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR020510; IcaD.
DR   TIGRFAMs; TIGR03932; PIA_icaD; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..101
FT                   /note="Poly-beta-1,6-N-acetyl-D-glucosamine synthesis
FT                   protein IcaD"
FT                   /id="PRO_0000084142"
FT   TRANSMEM        21..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        71..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   101 AA;  11951 MW;  CBAD3678D375B03B CRC64;
     MVKPRQRQYP TVTSYLNIVR ESLFITISGV FWMYCIVVMI VYIGTLINSQ MESVITIRIA
     LNVENTEIYK LFGWMSLFVL IIFIFFTFSL AFQKYKKGRD I