ID   ICAR_STAA8              Reviewed;         186 AA.
AC   Q9RQQ0; Q2FUV0;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=Biofilm operon icaADBC HTH-type negative transcriptional regulator IcaR;
DE   AltName: Full=Intercellular adhesion protein R;
GN   Name=icaR; OrderedLocusNames=SAOUHSC_03001;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10496925; DOI=10.1128/iai.67.10.5427-5433.1999;
RA   Cramton S.E., Gerke C., Schnell N.F., Nichols W.W., Goetz F.;
RT   "The intercellular adhesion (ica) locus is present in Staphylococcus aureus
RT   and is required for biofilm formation.";
RL   Infect. Immun. 67:5427-5433(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
CC   -!- FUNCTION: Represses transcription of the icaADBC operon necessary for
CC       biofilm production. {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Binding to the ica operator DNA involves two IcaR dimers
CC       and is highly cooperative. {ECO:0000250}.
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DR   EMBL; AF086783; AAD52054.1; -; Genomic_DNA.
DR   EMBL; CP000253; ABD31988.1; -; Genomic_DNA.
DR   PIR; A99975; A99975.
DR   RefSeq; WP_000653261.1; NZ_LS483365.1.
DR   RefSeq; YP_501450.1; NC_007795.1.
DR   AlphaFoldDB; Q9RQQ0; -.
DR   SMR; Q9RQQ0; -.
DR   STRING; 1280.SAXN108_2938; -.
DR   EnsemblBacteria; ABD31988; ABD31988; SAOUHSC_03001.
DR   GeneID; 3921483; -.
DR   KEGG; sao:SAOUHSC_03001; -.
DR   PATRIC; fig|93061.5.peg.2708; -.
DR   eggNOG; COG1309; Bacteria.
DR   HOGENOM; CLU_124987_0_0_9; -.
DR   OMA; ETNSYFA; -.
DR   PRO; PR:Q9RQQ0; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR041646; IcaR_C.
DR   Pfam; PF18665; TetR_C_37; 1.
DR   Pfam; PF00440; TetR_N; 1.
DR   PRINTS; PR00455; HTHTETR.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..186
FT                   /note="Biofilm operon icaADBC HTH-type negative
FT                   transcriptional regulator IcaR"
FT                   /id="PRO_0000070596"
FT   DOMAIN          1..59
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        22..41
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
SQ   SEQUENCE   186 AA;  21987 MW;  F1C76B0056C7ECAB CRC64;
     MKDKIIDNAI TLFSEKGYDG TTLDDIAKSV NIKKASLYYH FDSKKSIYEQ SVKCCFDYLN
     NIIMMNQNKS NYSIDALYQF LFEFIFDIEE RYIRMYVQLS NTPEEFSGNI YGQIQDLNQS
     LSKEIAKFYD ESKIKMTKED FQNLILLFLE SWYLKASFSQ KFGAVEESKS QFKDEVYSLL
     NIFLKK