ID   ICAR_STAAC              Reviewed;         186 AA.
AC   Q5HCN2;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Biofilm operon icaADBC HTH-type negative transcriptional regulator IcaR;
DE   AltName: Full=Intercellular adhesion protein R;
GN   Name=icaR; OrderedLocusNames=SACOL2688;
OS   Staphylococcus aureus (strain COL).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=COL;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), AND SUBUNIT.
RG   Center for structural genomics of infectious diseases (CSGID);
RT   "Crystal structure of icaR from Staphylococcus aureus, a member of the
RT   tetracycline repressor protein family.";
RL   Submitted (MAR-2009) to the PDB data bank.
CC   -!- FUNCTION: Represses transcription of the icaADBC operon necessary for
CC       biofilm production. {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|Ref.2}.
CC   -!- MISCELLANEOUS: Binding to the ica operator DNA involves two IcaR dimers
CC       and is highly cooperative. {ECO:0000250}.
CC   -!- MISCELLANEOUS: In strain COL, the gene icaC is interrupted by a natural
CC       frameshift.
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DR   EMBL; CP000046; AAW38685.1; -; Genomic_DNA.
DR   RefSeq; WP_000653261.1; NC_002951.2.
DR   PDB; 3GEU; X-ray; 1.90 A; A/B/C/D=1-186.
DR   PDBsum; 3GEU; -.
DR   AlphaFoldDB; Q5HCN2; -.
DR   SMR; Q5HCN2; -.
DR   EnsemblBacteria; AAW38685; AAW38685; SACOL2688.
DR   KEGG; sac:SACOL2688; -.
DR   HOGENOM; CLU_124987_0_0_9; -.
DR   OMA; ETNSYFA; -.
DR   EvolutionaryTrace; Q5HCN2; -.
DR   Proteomes; UP000000530; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR041646; IcaR_C.
DR   Pfam; PF18665; TetR_C_37; 1.
DR   Pfam; PF00440; TetR_N; 1.
DR   PRINTS; PR00455; HTHTETR.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..186
FT                   /note="Biofilm operon icaADBC HTH-type negative
FT                   transcriptional regulator IcaR"
FT                   /id="PRO_0000070597"
FT   DOMAIN          1..59
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        22..41
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   HELIX           1..20
FT                   /evidence="ECO:0007829|PDB:3GEU"
FT   HELIX           23..29
FT                   /evidence="ECO:0007829|PDB:3GEU"
FT   HELIX           34..37
FT                   /evidence="ECO:0007829|PDB:3GEU"
FT   TURN            38..40
FT                   /evidence="ECO:0007829|PDB:3GEU"
FT   HELIX           44..69
FT                   /evidence="ECO:0007829|PDB:3GEU"
FT   HELIX           74..87
FT                   /evidence="ECO:0007829|PDB:3GEU"
FT   HELIX           90..98
FT                   /evidence="ECO:0007829|PDB:3GEU"
FT   HELIX           99..101
FT                   /evidence="ECO:0007829|PDB:3GEU"
FT   HELIX           104..106
FT                   /evidence="ECO:0007829|PDB:3GEU"
FT   HELIX           110..126
FT                   /evidence="ECO:0007829|PDB:3GEU"
FT   TURN            131..133
FT                   /evidence="ECO:0007829|PDB:3GEU"
FT   HELIX           138..161
FT                   /evidence="ECO:0007829|PDB:3GEU"
FT   HELIX           165..180
FT                   /evidence="ECO:0007829|PDB:3GEU"
FT   HELIX           181..183
FT                   /evidence="ECO:0007829|PDB:3GEU"
SQ   SEQUENCE   186 AA;  21987 MW;  F1C76B0056C7ECAB CRC64;
     MKDKIIDNAI TLFSEKGYDG TTLDDIAKSV NIKKASLYYH FDSKKSIYEQ SVKCCFDYLN
     NIIMMNQNKS NYSIDALYQF LFEFIFDIEE RYIRMYVQLS NTPEEFSGNI YGQIQDLNQS
     LSKEIAKFYD ESKIKMTKED FQNLILLFLE SWYLKASFSQ KFGAVEESKS QFKDEVYSLL
     NIFLKK