ID   ICAR_STAAE              Reviewed;         186 AA.
AC   A6QKF4;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Biofilm operon icaADBC HTH-type negative transcriptional regulator IcaR;
DE   AltName: Full=Intercellular adhesion protein R;
GN   Name=icaR; OrderedLocusNames=NWMN_2564;
OS   Staphylococcus aureus (strain Newman).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=426430;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Newman;
RX   PubMed=17951380; DOI=10.1128/jb.01000-07;
RA   Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.;
RT   "Genome sequence of Staphylococcus aureus strain Newman and comparative
RT   analysis of staphylococcal genomes: polymorphism and evolution of two major
RT   pathogenicity islands.";
RL   J. Bacteriol. 190:300-310(2008).
RN   [2]
RP   FUNCTION AS ICA OPERON REPRESSOR, AND DISRUPTION PHENOTYPE.
RX   PubMed=15060048; DOI=10.1128/jb.186.8.2449-2456.2004;
RA   Jefferson K.K., Pier D.B., Goldmann D.A., Pier G.B.;
RT   "The teicoplanin-associated locus regulator (TcaR) and the intercellular
RT   adhesin locus regulator (IcaR) are transcriptional inhibitors of the ica
RT   locus in Staphylococcus aureus.";
RL   J. Bacteriol. 186:2449-2456(2004).
CC   -!- FUNCTION: Represses transcription of the icaADBC operon necessary for
CC       biofilm production. {ECO:0000269|PubMed:15060048}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Cells display an increase of ica transcription of
CC       about 100-fold, and poly-N-acetylglucosamine polysaccharide (PNAG)
CC       synthesis about 10-fold. Adherence to polystyrene microtiter wells also
CC       increases. Double deletion of icaR and tcaR increases ica transcription
CC       about 500-fold, and poly-N-acetylglucosamine polysaccharide (PNAG)
CC       synthesis about 100-fold. Adherence is significantly greater than that
CC       of the single icaR mutant. {ECO:0000269|PubMed:15060048}.
CC   -!- MISCELLANEOUS: Binding to the ica operator DNA involves two IcaR dimers
CC       and is highly cooperative. {ECO:0000250}.
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DR   EMBL; AP009351; BAF68836.1; -; Genomic_DNA.
DR   RefSeq; WP_000653261.1; NZ_CP023390.1.
DR   AlphaFoldDB; A6QKF4; -.
DR   SMR; A6QKF4; -.
DR   EnsemblBacteria; BAF68836; BAF68836; NWMN_2564.
DR   KEGG; sae:NWMN_2564; -.
DR   HOGENOM; CLU_124987_0_0_9; -.
DR   OMA; ETNSYFA; -.
DR   Proteomes; UP000006386; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR041646; IcaR_C.
DR   Pfam; PF18665; TetR_C_37; 1.
DR   Pfam; PF00440; TetR_N; 1.
DR   PRINTS; PR00455; HTHTETR.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..186
FT                   /note="Biofilm operon icaADBC HTH-type negative
FT                   transcriptional regulator IcaR"
FT                   /id="PRO_0000317038"
FT   DOMAIN          1..59
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        22..41
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
SQ   SEQUENCE   186 AA;  21987 MW;  F1C76B0056C7ECAB CRC64;
     MKDKIIDNAI TLFSEKGYDG TTLDDIAKSV NIKKASLYYH FDSKKSIYEQ SVKCCFDYLN
     NIIMMNQNKS NYSIDALYQF LFEFIFDIEE RYIRMYVQLS NTPEEFSGNI YGQIQDLNQS
     LSKEIAKFYD ESKIKMTKED FQNLILLFLE SWYLKASFSQ KFGAVEESKS QFKDEVYSLL
     NIFLKK