ICAR_STAAS
ID ICAR_STAAS Reviewed; 186 AA.
AC Q6G609;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Biofilm operon icaADBC HTH-type negative transcriptional regulator IcaR;
DE AltName: Full=Intercellular adhesion protein R;
GN Name=icaR; OrderedLocusNames=SAS2551;
OS Staphylococcus aureus (strain MSSA476).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=282459;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MSSA476;
RX PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT for the rapid evolution of virulence and drug resistance.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC -!- FUNCTION: Represses transcription of the icaADBC operon necessary for
CC biofilm production. {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- MISCELLANEOUS: Binding to the ica operator DNA involves two IcaR dimers
CC and is highly cooperative. {ECO:0000250}.
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DR EMBL; BX571857; CAG44368.1; -; Genomic_DNA.
DR RefSeq; WP_000653261.1; NC_002953.3.
DR AlphaFoldDB; Q6G609; -.
DR SMR; Q6G609; -.
DR KEGG; sas:SAS2551; -.
DR HOGENOM; CLU_124987_0_0_9; -.
DR OMA; ETNSYFA; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001647; HTH_TetR.
DR InterPro; IPR041646; IcaR_C.
DR Pfam; PF18665; TetR_C_37; 1.
DR Pfam; PF00440; TetR_N; 1.
DR PRINTS; PR00455; HTHTETR.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS50977; HTH_TETR_2; 1.
PE 3: Inferred from homology;
KW DNA-binding; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..186
FT /note="Biofilm operon icaADBC HTH-type negative
FT transcriptional regulator IcaR"
FT /id="PRO_0000070601"
FT DOMAIN 1..59
FT /note="HTH tetR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT DNA_BIND 22..41
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
SQ SEQUENCE 186 AA; 21987 MW; F1C76B0056C7ECAB CRC64;
MKDKIIDNAI TLFSEKGYDG TTLDDIAKSV NIKKASLYYH FDSKKSIYEQ SVKCCFDYLN
NIIMMNQNKS NYSIDALYQF LFEFIFDIEE RYIRMYVQLS NTPEEFSGNI YGQIQDLNQS
LSKEIAKFYD ESKIKMTKED FQNLILLFLE SWYLKASFSQ KFGAVEESKS QFKDEVYSLL
NIFLKK