ICA_APICA
ID ICA_APICA Reviewed; 223 AA.
AC Q5EF78; Q1HHN7;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 25-MAY-2022, entry version 36.
DE RecName: Full=Icarapin;
DE AltName: Full=Venom protein 2;
DE AltName: Allergen=Api m 10;
DE Flags: Precursor;
OS Apis mellifera carnica (Carniolan honeybee).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC Apis.
OX NCBI_TaxID=88217;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 33-46 AND 99-109,
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY (ISOFORM VARIANT 1).
RC TISSUE=Venom, and Venom duct;
RX PubMed=16112630; DOI=10.1016/j.bbapap.2005.07.017;
RA Peiren N., Vanrobaeys F., de Graaf D.C., Devreese B., Van Beeumen J.,
RA Jacobs F.J.;
RT "The protein composition of honeybee venom reconsidered by a proteomic
RT approach.";
RL Biochim. Biophys. Acta 1752:1-5(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, ALTERNATIVE SPLICING,
RP TISSUE SPECIFICITY, AND ALLERGEN (ISOFORMS VARIANT 1 AND VARIANT 2).
RC TISSUE=Venom duct;
RX PubMed=16914147; DOI=10.1016/j.febslet.2006.08.005;
RA Peiren N., de Graaf D.C., Brunain M., Bridts C.H., Ebo D.G., Stevens W.J.,
RA Jacobs F.J.;
RT "Molecular cloning and expression of icarapin, a novel IgE-binding bee
RT venom protein.";
RL FEBS Lett. 580:4895-4899(2006).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16112630,
CC ECO:0000269|PubMed:16914147}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=variant 1;
CC IsoId=Q5EF78-1; Sequence=Displayed;
CC Name=variant 2;
CC IsoId=Q5EF78-2; Sequence=VSP_040198;
CC -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC {ECO:0000269|PubMed:16914147}.
CC -!- ALLERGEN: Causes an allergic reaction in human.
CC -!- MISCELLANEOUS: [Isoform variant 2]: Fragment, sequence of 45-223.
CC {ECO:0000305}.
CC -!- CAUTION: Sequences and experimental results obtained from
CC PubMed:16112630 and PubMed:16914147 are obtained from A.mellifera
CC carnica and not A.mellifera. {ECO:0000305}.
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DR EMBL; AY897570; AAW81036.1; -; mRNA.
DR EMBL; DQ485318; ABF21077.1; -; mRNA.
DR EMBL; DQ485319; ABF21078.1; -; mRNA.
DR AlphaFoldDB; Q5EF78; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Allergen; Alternative splicing; Direct protein sequencing; Glycoprotein;
KW Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..223
FT /note="Icarapin"
FT /id="PRO_5000095342"
FT REGION 186..223
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 186..204
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 126
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 142
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 168
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 193
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 90..94
FT /note="SAISA -> T (in isoform variant 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_040198"
FT CONFLICT 140
FT /note="T -> A (in Ref. 2; AAW81036)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 223 AA; 24819 MW; 9BD51D084DFBFB28 CRC64;
MKTLGVLFIA AWFIACTHSF PGAHDEDSKE ERKNVDTVLV LPSIERDQMM AATFDFPSLS
FEDSDEGSNW NWNTLLRPNF LDGWYQTLQS AISAHMKKVR EQMAGILSRI PEQGVVNWNK
IPEGANTTST TKIIDGHVVT INETTYTDGS DDYSTLIRVR VIDVRPQNET ILTTVSSEAD
SDVTTLPTLI GKNETSTQSS RSVESVEDFD NEIPKNQGDV LTA
