ICCD_TALVA
ID ICCD_TALVA Reviewed; 292 AA.
AC A0A482NB13;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 05-JUN-2019, sequence version 1.
DT 03-AUG-2022, entry version 9.
DE RecName: Full=S-adenosyl-L-methionine-dependent Diels-Alderase iccD {ECO:0000303|PubMed:30905148};
DE EC=2.1.-.- {ECO:0000269|PubMed:30905148};
DE AltName: Full=C-methyltransferase iccD {ECO:0000303|PubMed:30905148};
DE Short=C-MT iccD {ECO:0000303|PubMed:30905148};
DE AltName: Full=Ilicicolin H biosynthesis cluster protein D {ECO:0000303|PubMed:30905148};
DE AltName: Full=Pericyclase iccD {ECO:0000303|PubMed:30905148};
GN Name=iccD {ECO:0000303|PubMed:30905148};
OS Talaromyces variabilis (Penicillium variabile).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Trichocomaceae; Talaromyces.
OX NCBI_TaxID=28576;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY,
RP BIOPHYSICOCHEMICAL PROPERTIES, AND PATHWAY.
RC STRAIN=HXQ-H-1;
RX PubMed=30905148; DOI=10.1021/jacs.9b02204;
RA Zhang Z., Jamieson C.S., Zhao Y.L., Li D., Ohashi M., Houk K.N., Tang Y.;
RT "Enzyme-catalyzed inverse-electron demand Diels-Alder reaction in the
RT biosynthesis of antifungal ilicicolin H.";
RL J. Am. Chem. Soc. 141:5659-5663(2019).
CC -!- FUNCTION: S-adenosyl-l-methionine-dependent Diels-Alderase; part of the
CC gene cluster that mediates the biosynthesis of ilicicolin H, a 4-
CC hydroxy-2-pyridonealkaloid that has potent and broad antifungal
CC activities by inhibiting the mitochondrial respiration chain
CC (PubMed:30905148). IccD catalyzes the Diels-Alder reaction that
CC converts the acyclic 2-pyridone intermediate to 8-epi-ilicicolin H
CC (PubMed:30905148). The biosynthesis of ilicicolin H starts with
CC formation of the tetramic acid by the hybrid PKS-NRPS synthetase iccA
CC with the partnering trans-enoyl reductase iccB since iccA lacks a
CC designated enoylreductase (ER) domain. The cytochrome P450
CC monooxygenase iccC then catalyzes the ring expansion of the tetramate
CC to the acyclic 2-pyridone. The pericyclase iccD further converts the
CC acyclic 2-pyridone into 8-epi-ilicicolin H. Finally, the epimerase iccE
CC converts 8-epi-ilicicolin H into ilicicolin H via epimerization. IccA
CC to iccE are sufficient for ilicicolin H biosynthesis and the roles of
CC the remaining enzymes, iccF, iccG and iccH within the pathway have
CC still to be determined (PubMed:30905148) (Probable).
CC {ECO:0000269|PubMed:30905148, ECO:0000305|PubMed:30905148}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-[(2E,4E,8S,10E,12Z)-4,8-dimethyltetradeca-2,4,10,12-
CC tetraenoyl]-4-hydroxy-5-(4-hydroxyphenyl)-1,2-dihydropyridin-2-one =
CC 8-epi-ilicicolin H; Xref=Rhea:RHEA:64556, ChEBI:CHEBI:155888,
CC ChEBI:CHEBI:155889; Evidence={ECO:0000269|PubMed:30905148};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64557;
CC Evidence={ECO:0000269|PubMed:30905148};
CC -!- COFACTOR:
CC Name=S-adenosyl-L-methionine; Xref=ChEBI:CHEBI:59789;
CC Evidence={ECO:0000305|PubMed:30905148};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=54 uM for the acyclic 2-pyridone {ECO:0000269|PubMed:30905148};
CC -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000269|PubMed:30905148}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. Erg6/SMT family. {ECO:0000305}.
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DR EMBL; MK539848; QBQ83708.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A482NB13; -.
DR SMR; A0A482NB13; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008168; F:methyltransferase activity; IEA:InterPro.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR013216; Methyltransf_11.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF08241; Methyltransf_11; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 1: Evidence at protein level;
KW Membrane; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..292
FT /note="S-adenosyl-L-methionine-dependent Diels-Alderase
FT iccD"
FT /id="PRO_0000449008"
FT TRANSMEM 240..262
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 292 AA; 32714 MW; D45BF7BDAE522E6A CRC64;
MASSEVFTSE EAAQRAIKTY YESQESRMGY ELVFGGTQHF GYYTPGTWSP FPIDKSLRRM
EEKLMGWLAL PAGSRILEAG CGVGHVALYL AKHGMRVTGV DIIDHHVEQA RRSAQKANLP
KDQIVIEKMD FERLEAIPSA SHDGAFTMQS LGHAFSAEKA LAGFFRVLKP GGRFALVEVE
RRPNAEADKK NPRLTEQLSM INVGTGMPTN ERSHDGFYKG LLEEAGFVDI ESRDISDNIL
PVVRMFYVVL LVPYLFVRLL GIEKHFVSML AGTAGYVGYD RWRFMVVTGR KP