位置:首页 > 蛋白库 > ICDHX_ARATH
ICDHX_ARATH
ID   ICDHX_ARATH             Reviewed;         416 AA.
AC   Q9SLK0;
DT   03-APR-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 149.
DE   RecName: Full=Peroxisomal isocitrate dehydrogenase [NADP];
DE            EC=1.1.1.42;
GN   Name=ICDH; OrderedLocusNames=At1g54340; ORFNames=F20D21.16;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia;
RA   Kihara T., Ito N., Koyama H., Hara T.;
RT   "Isolation of cDNA encoding NADP-specific isocitrate dehydrogenase from
RT   Arabidopsis thaliana.";
RL   Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=17951448; DOI=10.1105/tpc.107.050989;
RA   Reumann S., Babujee L., Ma C., Wienkoop S., Siemsen T., Antonicelli G.E.,
RA   Rasche N., Lueder F., Weckwerth W., Jahn O.;
RT   "Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting
RT   peptides, metabolic pathways, and defense mechanisms.";
RL   Plant Cell 19:3170-3193(2007).
CC   -!- FUNCTION: May be involved in response to oxidative stresses.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH;
CC         Xref=Rhea:RHEA:19629, ChEBI:CHEBI:15562, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.42;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000269|PubMed:17951448}.
CC   -!- SIMILARITY: Belongs to the isocitrate and isopropylmalate
CC       dehydrogenases family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF316501; AAK06592.1; -; mRNA.
DR   EMBL; AC005287; AAD25614.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE33082.1; -; Genomic_DNA.
DR   EMBL; BT025983; ABG25072.1; -; mRNA.
DR   PIR; A96585; A96585.
DR   RefSeq; NP_175836.1; NM_104312.3.
DR   AlphaFoldDB; Q9SLK0; -.
DR   SMR; Q9SLK0; -.
DR   BioGRID; 27100; 3.
DR   STRING; 3702.AT1G54340.1; -.
DR   PaxDb; Q9SLK0; -.
DR   PRIDE; Q9SLK0; -.
DR   ProteomicsDB; 228758; -.
DR   EnsemblPlants; AT1G54340.1; AT1G54340.1; AT1G54340.
DR   GeneID; 841875; -.
DR   Gramene; AT1G54340.1; AT1G54340.1; AT1G54340.
DR   KEGG; ath:AT1G54340; -.
DR   Araport; AT1G54340; -.
DR   TAIR; locus:2020128; AT1G54340.
DR   eggNOG; KOG1526; Eukaryota.
DR   HOGENOM; CLU_023296_1_1_1; -.
DR   InParanoid; Q9SLK0; -.
DR   OMA; PYLELNI; -.
DR   OrthoDB; 769322at2759; -.
DR   PhylomeDB; Q9SLK0; -.
DR   BioCyc; ARA:AT1G54340-MON; -.
DR   BRENDA; 1.1.1.42; 399.
DR   PRO; PR:Q9SLK0; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9SLK0; baseline and differential.
DR   Genevisible; Q9SLK0; AT.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; HDA:TAIR.
DR   GO; GO:0005777; C:peroxisome; HDA:TAIR.
DR   GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IBA:GO_Central.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006102; P:isocitrate metabolic process; IBA:GO_Central.
DR   GO; GO:0006739; P:NADP metabolic process; IBA:GO_Central.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR019818; IsoCit/isopropylmalate_DH_CS.
DR   InterPro; IPR004790; Isocitrate_DH_NADP.
DR   InterPro; IPR024084; IsoPropMal-DH-like_dom.
DR   PANTHER; PTHR11822; PTHR11822; 1.
DR   Pfam; PF00180; Iso_dh; 1.
DR   PIRSF; PIRSF000108; IDH_NADP; 1.
DR   SMART; SM01329; Iso_dh; 1.
DR   TIGRFAMs; TIGR00127; nadp_idh_euk; 1.
DR   PROSITE; PS00470; IDH_IMDH; 1.
PE   1: Evidence at protein level;
KW   Magnesium; Manganese; Metal-binding; NADP; Oxidoreductase; Peroxisome;
KW   Reference proteome; Stress response; Tricarboxylic acid cycle.
FT   CHAIN           1..416
FT                   /note="Peroxisomal isocitrate dehydrogenase [NADP]"
FT                   /id="PRO_0000421962"
FT   MOTIF           414..416
FT                   /note="Peroxisomal targeting signal"
FT                   /evidence="ECO:0000305"
FT   BINDING         77..79
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         79
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         84
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         96..102
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         111
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         134
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         253
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         261
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         276
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         311..316
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         329
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   SITE            141
FT                   /note="Critical for catalysis"
FT                   /evidence="ECO:0000250"
FT   SITE            213
FT                   /note="Critical for catalysis"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   416 AA;  47234 MW;  EEAAAC9E5C6B2FA5 CRC64;
     MEFEKIKVIN PVVEMDGDEM TRVIWKFIKD KLIFPFLELD IKYFDLGLPN RDFTDDKVTI
     ETAEATLKYN VAIKCATITP DEARVREFGL KKMWRSPNGT IRNILNGTVF REPIICRNIP
     RLVPGWTKPI CIGRHAFGDQ YRATDLIVNE PGKLKLVFEP SGSSQKTEFE VFNFTGGGVA
     LAMYNTDESI RAFAESSMYT AYQKKWPLYL STKNTILKIY DGRFKDIFQE VYEANWRSKY
     EAAGIWYEHR LIDDMVAYAM KSEGGYVWAC KNYDGDVQSD FLAQGYGSLG MMTSVLVCPD
     GKTIEAEAAH GTVTRHYRVH QKGGETSTNS IASIFAWSRG LAHRAKLDSN AALLSYTEKL
     EAACMGTVES GKMTKDLALL IHGAKVRRDQ YVNTEEFIDA VAWELKRRLL GNNSRL
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024