ICE1_DROME
ID ICE1_DROME Reviewed; 1395 AA.
AC Q9W1R4; Q8MRC0;
DT 01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Little elongation complex subunit 1;
DE AltName: Full=Interactor of little elongator complex ELL subunit 1;
GN Name=Ice1; ORFNames=CG13550;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND IDENTIFICATION IN THE LEC COMPLEX.
RX PubMed=22195968; DOI=10.1016/j.molcel.2011.12.008;
RA Smith E.R., Lin C., Garrett A.S., Thornton J., Mohaghegh N., Hu D.,
RA Jackson J., Saraf A., Swanson S.K., Seidel C., Florens L., Washburn M.P.,
RA Eissenberg J.C., Shilatifard A.;
RT "The little elongation complex regulates small nuclear RNA transcription.";
RL Mol. Cell 44:954-965(2011).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=23932780; DOI=10.1016/j.molcel.2013.07.003;
RA Hu D., Smith E.R., Garruss A.S., Mohaghegh N., Varberg J.M., Lin C.,
RA Jackson J., Gao X., Saraf A., Florens L., Washburn M.P., Eissenberg J.C.,
RA Shilatifard A.;
RT "The little elongation complex functions at initiation and elongation
RT phases of snRNA gene transcription.";
RL Mol. Cell 51:493-505(2013).
CC -!- FUNCTION: Component of the little elongation complex (LEC), a complex
CC required to regulate small nuclear RNA (snRNA) gene transcription by
CC RNA polymerase II and III (PubMed:22195968, PubMed:23932780).
CC Specifically acts as a scaffold protein that promotes the LEC complex
CC formation and recruitment and RNA polymerase II occupancy at snRNA
CC genes in subnuclear bodies (PubMed:23932780).
CC {ECO:0000269|PubMed:22195968, ECO:0000269|PubMed:23932780}.
CC -!- SUBUNIT: Component of the little elongation complex (LEC), at least
CC composed of Ell, Eaf, Ice1 and Ice2. {ECO:0000269|PubMed:22195968}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22195968,
CC ECO:0000269|PubMed:23932780}. Note=Associates to transcriptionally
CC active chromatin at snRNA genes.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9W1R4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9W1R4-2; Sequence=VSP_056746, VSP_056747;
CC -!- SIMILARITY: Belongs to the ICE1 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF46989.2; -; Genomic_DNA.
DR EMBL; AY121673; AAM52000.1; -; mRNA.
DR EMBL; BT150380; AGW52190.1; -; mRNA.
DR RefSeq; NP_611786.1; NM_137942.2. [Q9W1R4-1]
DR AlphaFoldDB; Q9W1R4; -.
DR BioGRID; 63302; 4.
DR IntAct; Q9W1R4; 4.
DR STRING; 7227.FBpp0071919; -.
DR PaxDb; Q9W1R4; -.
DR PRIDE; Q9W1R4; -.
DR EnsemblMetazoa; FBtr0072010; FBpp0071919; FBgn0034853. [Q9W1R4-1]
DR GeneID; 37701; -.
DR KEGG; dme:Dmel_CG13550; -.
DR UCSC; CG13550-RA; d. melanogaster. [Q9W1R4-1]
DR CTD; 23379; -.
DR FlyBase; FBgn0034853; Ice1.
DR VEuPathDB; VectorBase:FBgn0034853; -.
DR eggNOG; ENOG502S8KT; Eukaryota.
DR HOGENOM; CLU_004489_0_0_1; -.
DR InParanoid; Q9W1R4; -.
DR OMA; FNMANCD; -.
DR PhylomeDB; Q9W1R4; -.
DR SignaLink; Q9W1R4; -.
DR BioGRID-ORCS; 37701; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37701; -.
DR PRO; PR:Q9W1R4; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034853; Expressed in egg chamber and 24 other tissues.
DR Genevisible; Q9W1R4; DM.
DR GO; GO:0000791; C:euchromatin; IMP:UniProtKB.
DR GO; GO:0032783; C:super elongation complex; IPI:FlyBase.
DR GO; GO:0008023; C:transcription elongation factor complex; IDA:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; IDA:FlyBase.
DR GO; GO:0042795; P:snRNA transcription by RNA polymerase II; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..1395
FT /note="Little elongation complex subunit 1"
FT /id="PRO_0000430424"
FT REGION 175..212
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 452..496
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 561..588
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 658..680
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 707..786
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 841..872
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 908..936
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 748..778
FT /evidence="ECO:0000255"
FT COMPBIAS 179..207
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 476..494
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 659..680
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 759..776
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 638..639
FT /note="PN -> QI (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_056746"
FT VAR_SEQ 640..1395
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_056747"
SQ SEQUENCE 1395 AA; 157467 MW; E2AB254105F9923D CRC64;
MNLDEFSDFN IDQLLGPSAP LQDALVAPYK QVSLPRSNQD VYAKRSRLAE RIAQNDELIR
QVKQLQAQNK QLADLQRTAQ EVTDLYQKEK QQRIELEKRT KQIGERCGQL EKELDVQVGN
CENLQEQLQV RGLPVEAKDV LSILMQFSQR LGDDCGLLRR DQNIMKKLRE HCKTIDVSVP
TPKSPNSRSK RKAHQPGVNQ STQTDEEPAD PKPALCSVAV QVEGLIETRN QATQHKNTTT
TRGTTTASFI KHHDVGTCFP EPKPLPNIRQ ILDEMLSWRD DVVIEPMSPL SDLQQELQLE
DVPTNASVAT CTTLCDIHRE IDFVTDLPTQ IKVSASRPPS RTMLDSVKEE ARSSRELAKE
LFNFLPQNQS CLTNLPPQAF EELWQVFGQM VLALLQRRSN PSMATPPSVS QADFTRWLYE
LYEGTENQTE QTSNGSTSKR DFATSTECMD TGTDPIIQSP NISHEGHVTP IRLPSKPKER
KRKSKKRKAA ATPKPIAKRK CLEMETNNEL EVEREQTPET AIQFLSNLET FNMANCDNLE
MELDEEELYL LQLTSNAKQN ENKGNVRVQD PDDLKSPAQT SKFPKSENHL PAVLKETASS
LKGYDEKQCP FPDKTEELMH KKTFEIQENI GSLTELPPNI DISAYPVSMA EVRIVSSNKP
SEMEVNASEG TDSHLDKSVS NLDTGSMSLF GSDSDVESEY SIEQMAPELS DSDDTFDNSE
EDCRSADEPS NSKKIRNSSL FGSESESDES EERALEADDL TEAEAKVEEK ESEQKSSDVA
PAASSGKLLV VEADLVTKTQ PLFVEKLDLE NTQHTSTCTN PKETDESEDE HGLVIDEQIF
ISQPEEPPLT VPIAAKRRRT QSELKTLSPP GEVRLTRQRA KQLLDEQKSG PEKGLSLVEQ
IRRQLKKAIN KSEPFKKDST HDSKKDSTHD LKPLEHEDEL KAMQLGTNFE PIKLSCSISE
ESPASPPACE PMDELDPPPI EIPLEQAACT RKDDQHKSIV QHVLKMDTGL EKLVEANRKT
LGKSQPQLCA SIGKYLQENM QLESTCSDLA MDIYKVTKSE AVIVNAMITV ICKIGLDDGP
VERLLNALKY LNFSQRFLAE LEERLFRNTK ERPATELALN YVRLYLKATA LQATMSAGYE
NPARLLLAKI LYHFDQDMPP LVMEVLRQFP TALPHREQRE YDNSDALITV IKHLLMNRQY
DMQDPNGAER LLLSKLRFEY HFQPYEPSKQ QVLENLVGKL KAGREEELGY AFALFCRRSP
HLKVVESVVG EHLMPLATSY CDLAAQNELY DARLGLLLHC ISLVLKPLPL DTDISAFVGF
LKRLLVAVPR SGVQLAAVKA SLRLQRFGFK YTLDALKDYR PNYELDPLTR AMIRCFAKRR
RHFRHVAATG RRTEI
