ICEF1_MOUSE
ID ICEF1_MOUSE Reviewed; 406 AA.
AC Q5DU31; Q3TZY2; Q8CAP9;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Interactor protein for cytohesin exchange factors 1;
GN Name=Ipcef1; Synonyms=Kiaa0403;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 227-406 (ISOFORM 1).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Spleen, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164 (ISOFORM 2), AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Enhances the promotion of guanine-nucleotide exchange by
CC PSCD2 on ARF6 in a concentration-dependent manner. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with guanine-nucleotide exchange factors PSCD1,
CC PSCD2, PSCD3 and PSCD4. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cell membrane
CC {ECO:0000250}. Note=Translocated with PSCD2 to the plasma membrane upon
CC epidermal growth factor (EGF) stimulation. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q5DU31-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5DU31-2; Sequence=VSP_032683;
CC Name=3;
CC IsoId=Q5DU31-3; Sequence=VSP_032684, VSP_032685;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC29953.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAD90405.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAE34075.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK157384; BAE34075.1; ALT_INIT; mRNA.
DR EMBL; AK038257; BAC29953.1; ALT_INIT; mRNA.
DR EMBL; AK220339; BAD90405.1; ALT_INIT; mRNA.
DR EMBL; AC155718; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC164171; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS56688.1; -. [Q5DU31-1]
DR CCDS; CCDS83676.1; -. [Q5DU31-2]
DR RefSeq; NP_001164271.1; NM_001170800.1. [Q5DU31-1]
DR RefSeq; NP_001334154.1; NM_001347225.1. [Q5DU31-2]
DR AlphaFoldDB; Q5DU31; -.
DR SMR; Q5DU31; -.
DR BioGRID; 236068; 2.
DR STRING; 10090.ENSMUSP00000101242; -.
DR iPTMnet; Q5DU31; -.
DR PhosphoSitePlus; Q5DU31; -.
DR EPD; Q5DU31; -.
DR MaxQB; Q5DU31; -.
DR PaxDb; Q5DU31; -.
DR PRIDE; Q5DU31; -.
DR ProteomicsDB; 273259; -. [Q5DU31-1]
DR ProteomicsDB; 273260; -. [Q5DU31-2]
DR ProteomicsDB; 273261; -. [Q5DU31-3]
DR Antibodypedia; 33399; 132 antibodies from 18 providers.
DR DNASU; 320495; -.
DR Ensembl; ENSMUST00000078070; ENSMUSP00000077215; ENSMUSG00000064065. [Q5DU31-2]
DR Ensembl; ENSMUST00000105617; ENSMUSP00000101242; ENSMUSG00000064065. [Q5DU31-1]
DR GeneID; 320495; -.
DR KEGG; mmu:320495; -.
DR UCSC; uc007efr.1; mouse. [Q5DU31-3]
DR UCSC; uc007efs.1; mouse. [Q5DU31-2]
DR UCSC; uc011wzm.1; mouse. [Q5DU31-1]
DR CTD; 26034; -.
DR MGI; MGI:2444159; Ipcef1.
DR VEuPathDB; HostDB:ENSMUSG00000064065; -.
DR eggNOG; KOG1738; Eukaryota.
DR GeneTree; ENSGT00940000154428; -.
DR HOGENOM; CLU_051850_0_0_1; -.
DR InParanoid; Q5DU31; -.
DR OMA; ADTHCLA; -.
DR OrthoDB; 1121556at2759; -.
DR TreeFam; TF326495; -.
DR BioGRID-ORCS; 320495; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Ipcef1; mouse.
DR PRO; PR:Q5DU31; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q5DU31; protein.
DR Bgee; ENSMUSG00000064065; Expressed in animal zygote and 99 other tissues.
DR ExpressionAtlas; Q5DU31; baseline and differential.
DR Genevisible; Q5DU31; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0019904; F:protein domain specific binding; ISO:MGI.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR Pfam; PF00169; PH; 1.
DR SMART; SM00233; PH; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cytoplasm; Membrane; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..406
FT /note="Interactor protein for cytohesin exchange factors 1"
FT /id="PRO_0000326631"
FT DOMAIN 13..112
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 120..173
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 253..285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 383..406
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..173
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 269..285
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 151
FT /note="V -> VVRVD (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_032683"
FT VAR_SEQ 276..298
FT /note="EEIKSSEDDEMEKLYKSLEQASL -> GKPGICLLFLDGEYLLGHCMILI
FT (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_032684"
FT VAR_SEQ 299..406
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_032685"
FT MOD_RES Q5DU31-2:164
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 406 AA; 45812 MW; 866733F4E55FFB84 CRC64;
MSRRRISCKD LGHADCQGWL YKKKEKGTFL SNKWKKFWVV LKGSSLYWYS NQMAEKADGF
VNLSDFTVER ASECKKKNAF KINHPQIKAF YFAAENLQEM NVWLNKLGFA VTHQESITKD
EECYSESEQE DPEVAVEAPP PPYASTTSSP VAAQWASSSS PKRRETSCSF SSLENTVKAP
SQFSSSGSKE RQSWHNIVNS SPATEDAGLP LTFAEQVHTL AFSEASNCQA PENNCITSEG
GLLNLLSSDD TSSLNNNKDH LTVPDRAAGS RMADREEIKS SEDDEMEKLY KSLEQASLSP
LGDRRPSTKK ELRKSFVKRC KNPSINEKLH KIRTLNSTLK CKEHDLAMIN QLLDDPKLTA
RKYREWKVMN TLLIQDIYQQ QAPQDPEVTP QEVMNPTSSD CVENSL
