ID   ICEN_ENTAG              Reviewed;        1258 AA.
AC   P16239;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Ice nucleation protein;
GN   Name=iceE;
OS   Enterobacter agglomerans (Erwinia herbicola) (Pantoea agglomerans).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Pantoea; Pantoea agglomerans group.
OX   NCBI_TaxID=549;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M1;
RX   PubMed=2515997; DOI=10.1016/0378-1119(89)90488-5;
RA   Warren G.J., Corotto L.V.;
RT   "The consensus sequence of ice nucleation proteins from Erwinia herbicola,
RT   Pseudomonas fluorescens and Pseudomonas syringae.";
RL   Gene 85:239-242(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (WARM ICE NUCLEUS-DEFICIENT MUTANTS).
RX   PubMed=8412688; DOI=10.1111/j.1365-2958.1993.tb01699.x;
RA   Gurian-Sherman D., Lindow S.E., Panopoulos N.J.;
RT   "Isolation and characterization of hydroxylamine-induced mutations in the
RT   Erwinia herbicola ice nucleation gene that selectively reduce warm
RT   temperature ice nucleation activity.";
RL   Mol. Microbiol. 9:383-391(1993).
CC   -!- FUNCTION: Ice nucleation proteins enable bacteria to nucleate
CC       crystallization in supercooled water.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane.
CC   -!- DOMAIN: Contains 126 imperfect repeats of a consensus octapeptide A-G-
CC       Y-G-S-T-X-T; further on a 16-residue and a regional 48-residue
CC       periodicity is superimposed.
CC   -!- MISCELLANEOUS: A structural model is suggested in which the ice
CC       nucleation protein displays a symmetry related to that of ice.
CC   -!- SIMILARITY: Belongs to the bacterial ice nucleation protein family.
CC       {ECO:0000305}.
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DR   EMBL; M26382; AAA24823.1; -; Genomic_DNA.
DR   PIR; JQ0188; JQ0188.
DR   AlphaFoldDB; P16239; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0050825; F:ice binding; IEA:UniProtKB-KW.
DR   InterPro; IPR000258; Ice_nucleatn.
DR   Pfam; PF00818; Ice_nucleation; 23.
DR   PRINTS; PR00327; ICENUCLEATN.
DR   PROSITE; PS00314; ICE_NUCLEATION; 45.
PE   3: Inferred from homology;
KW   Cell outer membrane; Ice nucleation; Membrane; Repeat.
FT   CHAIN           1..1258
FT                   /note="Ice nucleation protein"
FT                   /id="PRO_0000204020"
FT   REGION          162..1217
FT                   /note="Octapeptide periodicity"
FT   REGION          260..287
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          311..342
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          356..383
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          407..438
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          452..480
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1258 AA;  125085 MW;  590E8A130077FBD4 CRC64;
     MKEDKVLILR TCANNMADHG GIIWPLSGIV ECKYWKPVKG FENGLTGLIW GKGSDSPLSL
     HADARWVVAE VDADECIAIE THGWIKFPRA EVLHVGTKTS AMQFILHHRA DYVACTEMQA
     GPGSPDVTSE VKVGNRSLPV TDDIDATIES GSTQPTQTIE IATYGSTLSG THQSQLIAGY
     GSTETAGDSS TLIAGYGSTG TAGADSTLVA GYGSTQTAGE ESSQMAGYGS TQTGMKGSDL
     TAGYGSTGTA GDDSSLIAGY GSTQTAGEDS SLTAGYGSTQ TAQKGSDLTA GYGSTGTAGA
     DSSLIAGYGS TQTAGEESTQ TAGYGSTQTA QKGSDLTAGY GSTGTAGDDS SLIAGYGSTQ
     TAGEDSSLTA GYGSTQTAQK GSDLTAGYGS TGTAGADSSL IAGYGSTQTA GEESTQTAGY
     GSTQTAQKGS DLTAGYGSTG TAGDDSSLIA GYGSTQTAGE DSSLTAGYGS TQTAQKGSDL
     TAGYGSTSTA GYESSLIAGY GSTQTAGYGS TLTAGYGSTQ TAQNESDLIT GYGSTSTAGA
     NSSLIAGYGS TQTASYNSVL TAGYGSTQTA REGSDLTAGY GSTGTAGSDS SIIAGYGSTQ
     TASYHSSLTA GYGSTQTARE QSVLTTGYGS TSTAGADSSL IAGYGSTQTA GYNSILTAGY
     GSTQTAQEGS DLTAGYGSTS TAGADSSLIA GYGSTQTAGY NSILTAGYGS TQTAQEGSDL
     TSGYGSTSTA GADSSLIAGY GSTQTASYHS SLTAGYGSTQ TAREQSVLTT GYGSTSTAGA
     DSSLIAGYGS TQTAGYHSIL TAGYGSTQTA QERSDLTTGY GSTSTAGADS SLIAGYGSTQ
     TAGYNSILTA GYGSTQTAQE NSDLTTGYGS TSTAGYDSSL IAGYGSTQTA GYNSILTAGY
     GSTQTAQENS DLTTGYGSTS TAGYESSLIA GYGSTQTASF KSTLMAGYGS SQTAREQSSL
     TAGYGSTSMA GYDSSLIAGY GSTQTAGYQS TLTAGYGSTQ TAEHSSTLTA GYGSTATAGA
     DSSLIAGYGS SLTSGIRSFL TAGYGSTLIS GLRSVLTAGY GSSLISGRRS SLTAGYGSNQ
     IASHRSSLIA GPESTQITGN RSMLIAGKGS SQTAGYRSTL ISGADSVQMA GERGKLIAGA
     DSTQTAGDRS KLLAGNNSYL TAGDRSKLTA GNDCILMAGD RSKLTAGINS ILTAGCRSKL
     IGSNGSTLTA GENSVLIFRC WDGKRYTNVV AKTGKGGIEA DMPYQMDEDN NIVNKPEE