ID   ICEN_PANAN              Reviewed;        1034 AA.
AC   Q47879;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Ice nucleation protein InaU;
GN   Name=inaU;
OS   Pantoea ananas (Erwinia uredovora).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Pantoea.
OX   NCBI_TaxID=553;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=KUIN-3;
RX   PubMed=7764866; DOI=10.1271/bbb.58.762;
RA   Michigami Y., Watabe S., Abe K., Obata H., Arai S.;
RT   "Cloning and sequencing of an ice nucleation active gene of Erwinia
RT   uredovora.";
RL   Biosci. Biotechnol. Biochem. 58:762-764(1994).
CC   -!- FUNCTION: Ice nucleation proteins enable bacteria to nucleate
CC       crystallization in supercooled water.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane.
CC   -!- DOMAIN: Contains imperfect repeats of a consensus octapeptide A-G-Y-G-
CC       S-T-X-T; further on a 16-residue and a regional 48-residue periodicity
CC       is superimposed.
CC   -!- MISCELLANEOUS: A structural model is suggested in which the ice
CC       nucleation protein displays a symmetry related to that of ice.
CC   -!- SIMILARITY: Belongs to the bacterial ice nucleation protein family.
CC       {ECO:0000305}.
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DR   EMBL; D14992; BAA03636.1; -; Genomic_DNA.
DR   PIR; JC2143; JC2143.
DR   AlphaFoldDB; Q47879; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0050825; F:ice binding; IEA:UniProtKB-KW.
DR   InterPro; IPR000258; Ice_nucleatn.
DR   Pfam; PF00818; Ice_nucleation; 15.
DR   PRINTS; PR00327; ICENUCLEATN.
DR   PROSITE; PS00314; ICE_NUCLEATION; 34.
PE   3: Inferred from homology;
KW   Cell outer membrane; Ice nucleation; Membrane; Repeat.
FT   CHAIN           1..1034
FT                   /note="Ice nucleation protein InaU"
FT                   /id="PRO_0000204022"
FT   REGION          162..993
FT                   /note="Octapeptide periodicity"
FT   REGION          260..287
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          311..342
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          356..383
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          407..438
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          452..480
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          570..597
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1034 AA;  103378 MW;  FA222523D333EADD CRC64;
     MKEDKVLILR TCANNMADHG GIIWPLSGIV ECKYWKPVKG FENGLTGLIW GKGSDSPLSL
     HADARWVVAE VDADECIAIE THGWIKFPRA EVLHVGTKTS AMQFILHHRA DYVACTEMQA
     GPGAPDVTSE VKAGNRSLPV TDDIDATIES GSTQPTQTIE IATYGSTLSG THQSQLIAGY
     GSTETAGDSS TLIAGYGSTG TAGSDSTLVA GYGSTQTAGE ESSQMAGYGS TQTGMKGSDL
     TAGYGSTGTA GDDSSLIAGY GSTQTAGEDS SLTAGYGSTQ TAQKGSDLTA GYGSTGTAGA
     DSSLIAGYGS TQTAGEESTQ TAGYGSTQTA QKGSDLTAGY GSTGTAGDDS SLIAGYGSTQ
     TAGEDSSLTA GYGSTQTAQK GSDLTAGYGS TGTAGADSSL IAGYGSTQTA GEESTQTAGY
     GSTQTAQKGS DLTAGYGSTG TAGDDSSLIA GYGSTQTAGE DSSLTAGYGS TQTAQKGSDL
     TAGYGSTSTA GYESSLIAGY GSTQTAGYGS TLTAGYGSTQ TAQNESDLIT GYGSTSTAGA
     NSSLIAGYGS TQTASYNSVL TAGYGSTQTA REGSDLTAGY GSTQTAQENS DLTTGYGSTS
     TAGYDSSLIA GYGSTQTAGY HSILTAGYGS TQTAQERSDL TTGYGSTSTA GADSSLIAGY
     GSTQTAGYNS ILTAGYGSTQ TAQENSDLTT GYGSTSTAGY ESSLIAGYGS TQTASFKSTL
     MAGYGSSQTA REQSSLTAGY GSTSMAGYDS SLIAGYGSTQ TAGYQSTLTA GYGSTQTAEH
     SSTLTAGYGS TATAGADSSL IAGYGSSLTS GIRSFLTAGY GSTLISGLRS VLTAGYGSSL
     ISGRRSSLTA GYGSNQIASH RSSLIAGPES TQITGNRSML IAGKGSSQTA GYRSTLISGA
     DSVQMAGERG KLIAGADSTQ TAGDRSKLLA GNNSYLTAGD RSKLTAGNDC ILMAGDRSKL
     TAGINSILTA GCRSKLIGSN GSTLTAGENS VLIFRCWDGK RYTNVVAKTG KGGIEADMPY
     QMDEDNNIVN KPEE