ID   ICEN_PSEFL              Reviewed;        1210 AA.
AC   P09815;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Ice nucleation protein;
GN   Name=inaW;
OS   Pseudomonas fluorescens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=294;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3774551; DOI=10.1093/nar/14.20.8047;
RA   Warren G.J., Corotto L., Wolber P.;
RT   "Conserved repeats in diverged ice nucleation structural genes from two
RT   species of Pseudomonas.";
RL   Nucleic Acids Res. 14:8047-8060(1986).
CC   -!- FUNCTION: Ice nucleation proteins enable bacteria to nucleate
CC       crystallization in supercooled water.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Peripheral
CC       membrane protein {ECO:0000250}.
CC   -!- DOMAIN: Contains 122 imperfect repeats of a consensus octapeptide A-G-
CC       Y-G-S-T-L-T; further on a 16-residue and a regional 48-residue
CC       periodicity is superimposed.
CC   -!- MISCELLANEOUS: A structural model is suggested in which the ice
CC       nucleation protein displays a symmetry related to that of ice.
CC   -!- SIMILARITY: Belongs to the bacterial ice nucleation protein family.
CC       {ECO:0000305}.
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DR   EMBL; X04501; CAA28186.1; -; Genomic_DNA.
DR   PIR; A25547; A25547.
DR   AlphaFoldDB; P09815; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0050825; F:ice binding; IEA:UniProtKB-KW.
DR   InterPro; IPR000258; Ice_nucleatn.
DR   Pfam; PF00818; Ice_nucleation; 15.
DR   PRINTS; PR00327; ICENUCLEATN.
DR   PROSITE; PS00314; ICE_NUCLEATION; 45.
PE   3: Inferred from homology;
KW   Cell outer membrane; Ice nucleation; Membrane; Repeat.
FT   CHAIN           1..1210
FT                   /note="Ice nucleation protein"
FT                   /id="PRO_0000204023"
FT   REGION          165..1156
FT                   /note="Octapeptide periodicity"
SQ   SEQUENCE   1210 AA;  118881 MW;  7C4ADD6633F61080 CRC64;
     MKSEKVLVLR TCANNMTDHC GLVWPILGLV ECKFWEPTIK LENGLTGALW GQGSSAQLSM
     NADAKWVVCE VTMGDLIFLE NNEGVKFPRA EVVHVGTRSS ALGYISDNVS KHEACSSNLI
     EKFTFSDVKS ETRNISPALP VTVDNMPNGV NRSTTVRNTQ TLETAVYGST LTGANQSQLI
     AGYGSTETAG DSSTLIAGYG STGTSGSDSS IIAGYGSTGT AGSDSSLIAG YGSTQTAGGD
     SSLTAGYGST QTAQVGSNLT AGYGSTGTAG PDSSLIAGYG STQTAGGESS LTAGYGSTQT
     AQVGSDLTAG YGSTGTAGSD SSLIAGYGST QTAGGDSSLT AGYGSTQTAQ VGSNLTAGYG
     STGTAGPDSS LIAGYGSTQT AGGESSLTAG YGSTQTAQVG SDLTAGYGST GTAGSDSSLI
     AGYGSTQTAG GESSLTAGYG STQTAQVGSD LTAGYGSTGT AGSDSSLIAG YGSTQTAGGD
     SSLTAGYGST QTAQVGSDLT AGYGSTGTAG SDSSLIAGYG STQTAGGDSS LTAGYGSTQT
     AQVGSDLTAG YGSTGTAGSD SSLIAGYGST QTAGGDSSLT AGYGSTQTAQ MGSNLTAGYG
     STGTAGSDSS LIAGYGSTQT AGGDSSLTAG YGSTQTAGHG SILTAGYGST QTAQEGSSLT
     AGYGSTSTAG PESSLIAGYG STQTAGHEST LTAGYGSTQT AQEDSSLTAG YGSTSTAGFN
     SSLIAGYGST QTSGYESILT AGYGSTQTAQ DNSSLTTGYG STSTAGYQSS LIAGYGSTQT
     AGYESTLTAG YGSCQTAQEQ SWLTTGYGST STAGYESRLI AGYGSTQTAG YKSILTAGYG
     STQTAQEESS LTAGYGSTST AGYASSLIAG YGSTQTAGYD SILTAGYGST LTALDSSTLT
     AGYGSTETAG FGSSLMAGYG SSQIAGYGST LTAGYGSTQM AERDSTLTAG YGSTGTAGQD
     SSLIAGYGSS LTSGMRSYLT AGYGSTLISG LQSVLTAGYG SSLTSGIRSS LTAGYGSNQI
     ASHKSSLIAG HESTQIAGHK SMLIAGKGSS QTAGSRSTLI AGANSVQMAG DRSRLTAGAN
     SIQTAGDRSK LLAGSNSYLT AGDRSKLTAG DDCVLMAGDR SKLTAGKNCV LTAGADSRLI
     GSLGSTLSGG ENSTLIFRCW DGKRYTNVVV KTGTDEVEAD VPYQIDEDSN VLIKAEDNSD
     TPVDQSQIQP