ID   ICEN_PSESY              Reviewed;        1200 AA.
AC   P06620;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Ice nucleation protein;
GN   Name=inaZ;
OS   Pseudomonas syringae pv. syringae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=S203;
RA   Green R.L., Warren G.J.;
RT   "Physical and functional repetition in a bacterial ice nucleation gene.";
RL   Nature 317:645-648(1985).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=2520825; DOI=10.1094/mpmi-2-262;
RA   Lindow S.E., Lahue E., Govindarajan A.G., Panopoulos N.J., Gies D.;
RT   "Localization of ice nucleation activity and the iceC gene product in
RT   Pseudomonas syringae and Escherichia coli.";
RL   Mol. Plant Microbe Interact. 2:262-272(1989).
RN   [3]
RP   3D-STRUCTURE MODELING OF 490-535.
RX   PubMed=8355267; DOI=10.1006/jmbi.1993.1424;
RA   Kajava A.V., Lindow S.E.;
RT   "A model of the three-dimensional structure of ice nucleation proteins.";
RL   J. Mol. Biol. 232:709-717(1993).
RN   [4]
RP   BIOTECHNOLOGICAL RELEVANCE.
RX   PubMed=1760850; DOI=10.3109/07388559109069185;
RA   Margaritis A., Bassi A.S.;
RT   "Principles and biotechnological applications of bacterial ice
RT   nucleation.";
RL   Crit. Rev. Biotechnol. 11:277-295(1991).
CC   -!- FUNCTION: Ice nucleation proteins enable bacteria to nucleate
CC       crystallization in supercooled water.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000269|PubMed:2520825}.
CC   -!- DOMAIN: Contains 122 imperfect repeats of a consensus octapeptide A-G-
CC       Y-G-S-T-L-T; further on a 16-residue and a regional 48-residue
CC       periodicity is superimposed.
CC   -!- BIOTECHNOLOGY: Used by snowmaking industry to promote ice nucleation
CC       for artificial snow and economize water. Sold under the name Snomax by
CC       York. {ECO:0000269|PubMed:1760850}.
CC   -!- MISCELLANEOUS: A structural model is suggested in which the ice
CC       nucleation protein displays a symmetry related to that of ice.
CC   -!- SIMILARITY: Belongs to the bacterial ice nucleation protein family.
CC       {ECO:0000305}.
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DR   EMBL; X03035; CAA26837.1; -; Genomic_DNA.
DR   PIR; A24405; SNPSO.
DR   PDB; 6M9I; EM; 0.90 A; A=899-904.
DR   PDB; 6M9J; EM; 0.90 A; A=899-904.
DR   PDBsum; 6M9I; -.
DR   PDBsum; 6M9J; -.
DR   AlphaFoldDB; P06620; -.
DR   SMR; P06620; -.
DR   TCDB; 9.B.133.1.1; the ice nucleation protein secretion system (inp-ss) family.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0050825; F:ice binding; IEA:UniProtKB-KW.
DR   InterPro; IPR000258; Ice_nucleatn.
DR   Pfam; PF00818; Ice_nucleation; 12.
DR   PRINTS; PR00327; ICENUCLEATN.
DR   PROSITE; PS00314; ICE_NUCLEATION; 40.
PE   1: Evidence at protein level;
KW   3D-structure; Cell outer membrane; Ice nucleation; Membrane; Repeat.
FT   CHAIN           1..1200
FT                   /note="Ice nucleation protein"
FT                   /id="PRO_0000204024"
FT   REGION          176..1151
FT                   /note="Octapeptide periodicity"
SQ   SEQUENCE   1200 AA;  118588 MW;  E3BEAD4BEA67ABB0 CRC64;
     MNLDKALVLR TCANNMADHC GLIWPASGTV ESRYWQSTRR HENGLVGLLW GAGTSAFLSV
     HADARWIVCE VAVADIISLE EPGMVKFPRA EVVHVGDRIS ASHFISARQA DPASTSTSTL
     TPMPTAIPTP MPAVASVTLP VAEQARHEVF DVASVSAAAA PVNTLPVTTP QNVQTATYGS
     TLSGDNHSRL IAGYGSNETA GNHSDLIAGY GSTGTAGSDS WLVAGYGSTQ TAGGDSALTA
     GYGSTQTARE GSNLTAGYGS TGTAGSDSSL IAGYGSTQTS GGDSSLTAGY GSTQTAQEGS
     NLTAGYGSTG TAGSDSSLIA GYGSTQTSGG DSSLTAGYGS TQTAQEGSNL TAGYGSTGTA
     GVDSSLIAGY GSTQTSGSDS ALTAGYGSTQ TAQEGSNLTA GYGSTGTAGS DSSLIAGYGS
     TQTSGSDSSL TAGYGSTQTA QEGSILTAGY GSTGTAGVDS SLIAGYGSTQ TSGSDSALTA
     GYGSTQTAQE GSNLTAGYGS TGTAGADSSL IAGYGSTQTS GSESSLTAGY GSTQTAREGS
     TLTAGYGSTG TAGADSSLIA GYGSTQTSGS ESSLTAGYGS TQTAQQGSVL TSGYGSTQTA
     GAASNLTTGY GSTGTAGHES FIIAGYGSTQ TAGHKSILTA GYGSTQTARD GSDLIAGYGS
     TGTAGSGSSL IAGYGSTQTA SYRSMLTAGY GSTQTAREHS DLVTGYGSTS TAGSNSSLIA
     GYGSTQTAGF KSILTAGYGS TQTAQERTSL VAGYGSTSTA GYSSSLIAGY GSTQTAGYES
     TLTAGYGSTQ TAQENSSLTT GYGSTSTAGY SSSLIAGYGS TQTAGYESTL TAGYGSTQTA
     QERSDLVTGY GSTSTAGYAS SLIAGYGSTQ TAGYESTLTA GYGSTQTAQE NSSLTTGYGS
     TSTAGFASSL ISGYGSTQTA GYKSTLTAGY GSTQTAEYGS SLTAGYGSTA TAGQDSSLIA
     GYGSSLTSGI RSFLTAGYGS TLIAGLRSVL IAGYGSSLTS GVRSTLTAGY GSNQIASYGS
     SLIAGHESIQ VAGNKSMLIA GKGSSQTAGF RSTLIAGAGS VQLAGDRSRL IAGADSNQTA
     GDRSKLLAGN NSYLTAGDRS KLTGGHDCTL MAGDQSRLTA GKNSVLTAGA RSKLIGSEGS
     TLSAGEDSIL IFRLWDGKRY RQLVARTGEN GVEADIPYYV NEDDDIVDKP DEDDDWIEVK