ID   ICEV_PSESX              Reviewed;        1196 AA.
AC   O33479;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Ice nucleation protein;
GN   Name=inaV;
OS   Pseudomonas syringae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=317;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=INA5;
RX   PubMed=9323042; DOI=10.1016/s0014-5793(97)01079-x;
RA   Schmid D., Pridmore D., Capitani G., Battistutta R., Neeser J.-R., Jann A.;
RT   "Molecular organisation of the ice nucleation protein InaV from Pseudomonas
RT   syringae.";
RL   FEBS Lett. 414:590-594(1997).
CC   -!- FUNCTION: Ice nucleation proteins enable bacteria to nucleate
CC       crystallization in supercooled water.
CC   -!- SUBUNIT: Membrane environment or aggregation seems to be required for
CC       ice nucleation activity.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Peripheral
CC       membrane protein {ECO:0000250}.
CC   -!- DOMAIN: Contains many imperfect repeats of a consensus octapeptide A-G-
CC       Y-G-S-T-L-T; further on a 16-residue and a regional 48-residue
CC       periodicity is superimposed.
CC   -!- SIMILARITY: Belongs to the bacterial ice nucleation protein family.
CC       {ECO:0000305}.
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DR   EMBL; AJ001086; CAA04521.1; -; Genomic_DNA.
DR   AlphaFoldDB; O33479; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0050825; F:ice binding; IEA:UniProtKB-KW.
DR   InterPro; IPR000258; Ice_nucleatn.
DR   Pfam; PF00818; Ice_nucleation; 15.
DR   PRINTS; PR00327; ICENUCLEATN.
DR   PROSITE; PS00314; ICE_NUCLEATION; 42.
PE   3: Inferred from homology;
KW   Cell outer membrane; Ice nucleation; Membrane; Repeat.
FT   CHAIN           1..1196
FT                   /note="Ice nucleation protein"
FT                   /id="PRO_0000204026"
FT   REGION          172..1147
FT                   /note="Octapeptide periodicity"
FT   REGION          269..304
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          319..352
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          415..442
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1196 AA;  117992 MW;  C9E9974CB1731E68 CRC64;
     MNIDKALVLR TCANNMADHC GLIWPASGTV ESKYWQSTRR HENGLVGLLW GAGTSAFLSV
     HADARWKVCE VAVADIIGLE EPGMVKFPRA EVVHVGDRIS ASHFISARQA DPASTPTPTP
     TPMATPTPAA ANIALPVVEQ PSHEVFDVAL VSAAAPSVNT LPVTTPQNLQ TATYGSTLSG
     DNNSRLIAGY GSNETAGNHS DLIAGYGSTG TAGSDSSLVA GYGSTQTAGG DSALTAGYGS
     TQTAREGSNL TAGYGSTGTA GSDSSLIAGY GSTQTSGEDS SLTAGYGSTQ TAQEGSNLTA
     GYGSTGTAGS DSSLIAGYGS TQTSGGDSSL TAGYGSTQTA QEGSNLTSGY GSTGTAGADS
     SLIAGYGSTQ TSGSDSALTA GYGSTQTAQE GSNLTAGYGS TGTAGSDSSL IAGYGSTQTS
     GSDSSLTAGY GSTQTAQEGS NLTAGYGSTG TAGVDSSLIA GYGSTQTSGS DSALTAGYGS
     TQTAQEGSNL TAGYGSTGTA GADSSLIAGY GSTQTSGSES SLTAGYGSTQ TAREGSTLTA
     GYGSTGTAGA DSSLIAGYGS TQTSGSDSSL TAGYGSTQTA QQGSVLTSGY GSTQTAGAAS
     NLTTGYGSTG TAGHESFIIA GYGSTQTAGH KSILTAGYGS TQTARDGSDL VAGYGSTGTA
     GSGSSLIAGY GSTQTASYKS MLTAGYGSTQ TAREHSDLVA GYGSTSTAGS NSSLIAGYGS
     TQTAGFKSIM TAGYGSTQTA QERSDLVAGY GSTSTAGYSS SLIAGYGSTQ TAGYGSTLTT
     GYGSTQTAQE NSSLTTGYGS TSTAGYSSSL IAGYGSTQTA GYESTLTAGY GSTQTAQERS
     DLVTGYGSTS TAGYASSLIA GYGSTQTAGY ESTLTAGYGS TQTAQENSSL TTGYGSTSTA
     GFASSLIAGY GSTQTAGYKS TLTAGYGSTQ TAEYGSSLTA GYGSTATAGQ DSSLIAGYGS
     TLTSGIRSFL TAGYGSTLIA GLRSVLIAGY GSSLTSGIRS TLTAGYGSNQ IASYGSSLIA
     GHESIQVAGN KSMLIAGKGS SQTAGFRSTL IAGAGSVQLA GDRSRLIAGA DSNQTAGDRS
     KLLAGNNSYL TAGDRSKLTG GHDCTLMAGD QSRLTAGKNS ILTAGARSKL IGSEGSTLSA
     GEDSTLIFRL WDGKRYRQLV ARTGENGVEA DIPYYVNEDD DIVDKPDEDD DWIEVE