ICH_DROME
ID ICH_DROME Reviewed; 592 AA.
AC Q9VHJ6; A0AVW9; B7FNR4; T1T717;
DT 18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Ichor {ECO:0000303|PubMed:29309404, ECO:0000312|FlyBase:FBgn0286204};
GN Name=ich {ECO:0000303|PubMed:29309404, ECO:0000312|FlyBase:FBgn0286204};
GN ORFNames=CG11966 {ECO:0000312|FlyBase:FBgn0286204};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:ABK30924.1, ECO:0000312|EMBL:ACK77672.1, ECO:0000312|EMBL:AGT59571.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AGT59571.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AGT59571.1};
RA Carlson J., Booth B., Carlson J., Frise E., Kapadia B., Park S., Wan K.,
RA Yu C., Celniker S.;
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE,
RP AND MUTAGENESIS OF 107-GLN--ASP-592 AND HIS-582.
RX PubMed=29309404; DOI=10.1371/journal.pgen.1007146;
RA Rosa J.B., Metzstein M.M., Ghabrial A.S.;
RT "An Ichor-dependent apical extracellular matrix regulates seamless tube
RT shape and integrity.";
RL PLoS Genet. 14:E1007146-E1007146(2018).
CC -!- FUNCTION: Transcriptional activator. In tracheal terminal cells,
CC regulates the transcription of factors involved in the formation of a
CC mature apical extracellular matrix (aECM) which is essential for the
CC integrity and shape of seamless tubes. {ECO:0000269|PubMed:29309404}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:29309404}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryo from stage 10 in all
CC ectodermally-derived epithelia secreting cuticle. During germband
CC retraction, epidermal expression is strongest in the T2, T3, and A8
CC epidermal parasegments, but not detected during primary branching. Pan-
CC tracheal expression is first detected at stage 14 and continues during
CC later stages coinciding with lumen growth and cuticle deposition. In
CC addition to tracheal expression, expressed in epidermis, foregut, and
CC hindgut. {ECO:0000269|PubMed:29309404}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethal. RNAi-mediated knockdown in the
CC trachea of first instar larvae results in discontinuous tubes. RNAi-
CC mediated knockdown in the tracheal terminal cells of first instar larva
CC results in lumen devoid of taenidiae and occluded with disorganized
CC electron-dense material, pan-tracheal liquid clearance defect as well
CC as a breaks in the dorsal trunks. {ECO:0000269|PubMed:29309404}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACK77672.1; Type=Miscellaneous discrepancy; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAF54310.2; -; Genomic_DNA.
DR EMBL; AE014297; AGB95775.1; -; Genomic_DNA.
DR EMBL; BT029287; ABK30924.1; -; mRNA.
DR EMBL; BT053754; ACK77672.1; ALT_SEQ; mRNA.
DR EMBL; BT150203; AGT59571.1; -; mRNA.
DR RefSeq; NP_001262393.1; NM_001275464.1.
DR RefSeq; NP_649848.2; NM_141591.3.
DR AlphaFoldDB; Q9VHJ6; -.
DR SMR; Q9VHJ6; -.
DR IntAct; Q9VHJ6; 1.
DR STRING; 7227.FBpp0081492; -.
DR PaxDb; Q9VHJ6; -.
DR EnsemblMetazoa; FBtr0082014; FBpp0081492; FBgn0286204.
DR EnsemblMetazoa; FBtr0334329; FBpp0306438; FBgn0286204.
DR GeneID; 41069; -.
DR KEGG; dme:Dmel_CG11966; -.
DR UCSC; CG11966-RA; d. melanogaster.
DR CTD; 41069; -.
DR FlyBase; FBgn0286204; ich.
DR VEuPathDB; VectorBase:FBgn0286204; -.
DR eggNOG; KOG1721; Eukaryota.
DR HOGENOM; CLU_023063_0_0_1; -.
DR InParanoid; Q9VHJ6; -.
DR OMA; TEYVDMN; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q9VHJ6; -.
DR BioGRID-ORCS; 41069; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41069; -.
DR PRO; PR:Q9VHJ6; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0286204; Expressed in embryonic epidermis (Drosophila) and 20 other tissues.
DR GO; GO:0005634; C:nucleus; IMP:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1901148; P:gene expression involved in extracellular matrix organization; IMP:UniProtKB.
DR GO; GO:0035149; P:lumen formation, open tracheal system; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:FlyBase.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0060439; P:trachea morphogenesis; IMP:UniProtKB.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 1.
DR SMART; SM00355; ZnF_C2H2; 2.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 2.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..592
FT /note="Ichor"
FT /evidence="ECO:0000305"
FT /id="PRO_0000444696"
FT ZN_FING 536..558
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 564..586
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 114..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 343..377
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 416..439
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 459..527
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 343..362
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 484..523
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 107..592
FT /note="Missing: Loss of zinc fingers domains results in
FT defects in cuticle elasticity, reduced sclerotization of
FT the head skeleton and ventral epidermal denticles, and
FT disruption of multiple aspects of tube morphogenesis in
FT terminal cells including seamless tube growth, shape, and
FT integrity."
FT /evidence="ECO:0000269|PubMed:29309404"
FT MUTAGEN 582
FT /note="H->Y: Probable loss of zinc ion coordination in the
FT second zinc finger results in defects in cuticle
FT elasticity, reduced sclerotization of the head skeleton and
FT ventral epidermal denticles, and disruption of multiple
FT aspects of tube morphogenesis in terminal cells including
FT seamless tube growth, shape, and integrity."
FT /evidence="ECO:0000269|PubMed:29309404"
FT CONFLICT 19
FT /note="M -> T (in Ref. 3; ACK77672/ABK30924)"
FT /evidence="ECO:0000305"
FT CONFLICT 513
FT /note="S -> G (in Ref. 3; ACK77672/ABK30924)"
FT /evidence="ECO:0000305"
FT CONFLICT 584
FT /note="Q -> R (in Ref. 3; AGT59571)"
FT /evidence="ECO:0000305"
FT CONFLICT 585
FT /note="I -> M (in Ref. 3; ACK77672/ABK30924/AGT59571)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 592 AA; 62626 MW; EE92680B248DFC54 CRC64;
MKFCSMNGPS ESEVESLLMS PCWGPPQTSA QDQYLLDGHK LLLEHDLDSL PSDADQKNSL
DVLDNLLLNG SSLNALSDLK PLPPFTGYTG HLSINGISGH HYHAIAQRLP DESNNNYMQS
AYHPQNQSNP TSTTQSNGGS NSNSNNSNEH NIVSSSTCLP ESVLSGSGGG GGGNDACLAD
VKLFADSQLD SKLYSVADSC VMNGSGSGSA ANGNGSGPSI ATLTPIDQVA DSLHNSGVRI
YKDLTEYVDM NSIDDIAAII GSAIADTTVP NQLDKDDNND TRDSWMDLDA WIDGNCIQQE
SAKVLVSQQD SLGDFILPHS PLPMHASSST LQSLLSHGYM PLLQNRLQNG PPNGNSSGGG
GGANQGAGIK GDPQAPTSTS YCNELAAATS SSCSPPGSVV STTDNPNGLM INPRYLSNPT
NNQATGNLHQ QGGYSMQASG LSLKDNGLCS PDLLGNYPHT TTASTTGSEM RTGAPKAKRS
RSQKKSNQQQ QQQQQQQQQQ GDGGGQPTTP QMSAISPSGF SASDLSGLLG KEKPVHRCSI
CNRGFLNKSN IKVHLRTHTG EKPFRCDVCA KAFRQKAHLL KHQQIHKRIG RD
